Characterizations and validations of novel antibodies toward translational research

Proteomics - Clinical Applications

Volumn 4, Issue 6-7, 2010, Pages 618-625

  Williams, Reginald ^a   Chung, Joon Yong ^b   Ylaya, Kris ^a   Whiteley, Gordon ^b   Hewitt, Stephen M ^a,b  

^a NATIONAL CANCER INSTITUTE   (United States)

^b SAIC FREDERICK INC   (United States)

Author keywords

Antibody specificity; Immunohistochemistry; Protein array; Tissue microarray

Indexed keywords

MESSENGER RNA; MONOCLONAL ANTIBODY;

ANTIBODY SCREENING; ANTIBODY TITER; ARTICLE; CELL STRAIN; CONTROLLED STUDY; GENE EXPRESSION; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; MICROARRAY ANALYSIS; PRIORITY JOURNAL; PROTEIN MICROARRAY; PROTEOMICS; SIGNAL TRANSDUCTION; TISSUE MICROARRAY; VALIDATION STUDY;

ANTIBODIES, MONOCLONAL; BIOLOGICAL MARKERS; CELL LINE, TUMOR; GENOME, HUMAN; HUMANS; IMMUNOHISTOCHEMISTRY; PROTEIN ARRAY ANALYSIS; PROTEOME; TRANSLATIONAL RESEARCH;

EID: 77955815648     PISSN: 18628346     EISSN: 18628354     Source Type: Journal    
DOI: 10.1002/prca.200900186     Document Type: Article

References (17)

1. Lander, E. S., Linton, L. M., Birren, B., Nusbaum, C. et al., Initial sequencing and analysis of human genome. Nature 2001, 409, 860-921.
2. Wong, S. C., Chan, C. M., Ma, B. B., Lam, M. Y. et al., Advanced proteomic technologies for cancer biomarker discovery. Exp. Rev. Proteomics 2009, 6, 123-134.
3. Lueking, A., Possling, A., Huber, O., Beveridge, A. et al., A nonredundant human protein chip for antibody screening and serum profiling. Mol. Cell Proteomics 2003, 2, 1342-1349.
4. Michaud, G. A., Salcius, M., Zhou, F., Bangham, R. et al., Analyzing antibody specificity with whole proteome microarrays. Nat. Biotechnol. 2003, 21, 1509-1512.
5. MacBeath, G., Protein microarrays and proteomics. Nat. Genet. 2002, 32, 526-532.
6. Sheehan, K. M., Calvert, V. S., Kay, E. W., Lu, Y. et al., Use of reverse phase protein microarrays and reference standard development for molecular network analysis of metastic ovarian carcinoma. Mol. Cell Proteomics 2005, 4, 346-355.
7. Tibes, R., Qiu, Y., Lu, Y., Hennessy, B. et al., Reverse phase protein array: validation of novel proteomic technology and utility for analysis of primary leukemia specimens and hematopoietic stem cells. Mol. Cancer Ther. 2006, 5, 2512-2521.
8. Nilsson, P., Paavilainen, L., Larsson, K., Odling, J. et al., Towards a human proteome atlas: high-throughput generation of mono-specific antibodies for tissue profiling. Proteomics 2005, 5, 4327-4337.
9. Uhlen, M., Ingvarsson, J., Generation and validation of affinity reagents on a proteom-wide level. J. Mol. Recognit. 2009, 22, 57-64.
10. Tyers, M., Mann, M., From genomics to proteomics. Nature 2003, 422, 193-197.
11. Hanash, S., HUPO initiatives relevant to clinical proteomics. Mol. Cell Proteomics 2004, 3, 298-301.
12. Björling, E., Lindskog, C., Oksvold, P., Linne, J. et al., A webbased tool for in silico biomarker discovery based on tissuespecific protein profiles in normal and cancer tissues. Mol. Cell Proteomics 2008, 7, 825-844.
13. Ponten, F., Jirstrom, K., Uhlen, M., The human protein atlasa tool for pathology. J. Pathol. 2008, 216, 387-393.
14. Jögi, A., Brennan, D. J., Ryden, L., Magnusson, K. et al., Nuclear expression of RNA-binding protein RBM3 is associated with an improved clinical outcome in breast cancer. Mod. Pathol. 2009, 22, 1564-1574.
15. Chung, J. Y., Lee, S. J., Ylaya, K., Braunschweig, T. et al., A well-based reverse-phase protein array applicable to extracts from formalin-fixed paraffin-embedded tissue. Proteomics Clin. Appl. 2008, 2, 1539-1547.
16. Hewitt, S. M., Design, construction and use of tissue microarrays. Methods Mol. Biol. 2004, 264, 61-72.
17. Kononen, J., Bubendorf, L., Kallioniemi, A., Barlund, M. et al., Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat. Med. 1998, 4, 844-847.