The use of markers for correlative light electron microscopy

Protoplasma

Volumn 244, Issue 1, 2010, Pages 91-97

  Brown, Edward ^a   Verkade, Paul ^a,b  

^a UNIVERSITY OF BRISTOL   (United Kingdom)

^b SCHOOL OF MEDICAL SCIENCES   (United Kingdom)

Author keywords

Electron microscopy; Endocytosis; Gold; Live cell imaging; Quantum dots

Indexed keywords

BIOLOGICAL MARKER; EPIDERMAL GROWTH FACTOR; QUANTUM DOT; TRANSFERRIN;

ANIMAL; ELECTRON MICROSCOPY; HUMAN; METABOLISM; METHODOLOGY; MOLECULAR PROBE; REVIEW; ULTRASTRUCTURE;

ANIMALS; BIOLOGICAL MARKERS; EPIDERMAL GROWTH FACTOR; HUMANS; MICROSCOPY, ELECTRON; MOLECULAR PROBES; QUANTUM DOTS; TRANSFERRIN;

EID: 77955768782     PISSN: 0033183X     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00709-010-0165-1     Document Type: Review

References (17)

1. Brown E, Mantell J, Carter D, Tilly G, Verkade P (2009) Studying intracellular transport using high-pressure freezing and correlative light electron microscopy. Semin Cell Dev Biol 20: 910-919.
2. Futter CE, Pearse A, Hewlett LJ, Hopkins CR (1996) Multivesicular endosomes containing internalized EGF-EGF receptor complexes mature and the fuse directly with lysosomes. J Cell Biol 132(6): 1011-1023.
3. Gaietta G, Deerinck TJ, Adams SR, Bouwer J, Tour O, Laird DW, Sosinsky GE, Tsien RY, Ellisman MH (2002) Multicolor and electron microscopic imaging of connexin trafficking. Science 296: 503-507.
4. Giepmans BN (2008) Bridging fluorescence microscopy and electron microscopy. Histochem Cell Biol 130: 211-217.
5. Giepmans BN, Deerinck TJ, Smarr BL, Jones YZ, Ellisman MH (2005) Correlated light and electron microscopic imaging of multiple endogenous proteins using Quantum dots. Nat Meth 2: 743-749.
6. Kandela IK, Meyer DA, Oshel PE, Rosa-Molinar E, Albrecht RM (2003) Fluorescence quenching by colloidal heavy metals: implications for correlative fluorescence and electron microscopy studies. Microsc Microanal 9: 1194-1195.
7. Mironov AA, Polishchuk RS, Luini A (2000) Visualizing membrane traffic in vivo by combined video fluorescence and 3D electron microscopy. Trends Cell Biol 10: 349-453.
8. Morris RE, Ciraolo GM, Saelinger CB (1992) Validation of the biotinyl ligand-avidin-gold technique. J Histochem Cytochem 40(5): 711-721.
9. Muller-Reichert T, Srayko M, Hyman A, O'Toole ET, McDonald K (2007) Correlative light and electron microscopy of early Caenorhabditis elegans embryos in mitosis. Meth Cell Biol 79: 101-119.
10. Neutra MR, Ciechanover A, Owen LS, Lodish HF (1985) Intracellular transport of transferrin- and asialoorosomucoid-colloidal gold conjugates to lysosomes after receptor-mediated endocytosis. J Histochem Cytochem 33(10): 1134-1144.
11. Plitzko JM, Rigort A, Leis A (2009) Correlative cryo-light microscopy and cryo-electron tomography: from cellular territories to molecular landscapes. Curr Opin Biotechnol 20: 83-89.
12. Polishchuk RS, Polishchuk EV, Marra P, Alberti S, Buccione R, Luini A, Mironov AA (2000) Correlative light-electron microscopy reveals the tubular-saccular ultrastructure of carriers operating between Golgi apparatus and plasma membrane. J Cell Biol 148: 45-58.
13. Robinson JM, Takizawa T (2009) Correlative fluorescence and electron microscopy in tissues: immunocytochemistry. J Microsc 235: 259-272.
14. van Rijnsoever C, Oorschot V, Klumperman J (2008) Correlative light-electron microscopy (CLEM) combining live-cell imaging and immunolabeling of ultrathin cryosections. Nat Methods 5: 973-980.
15. van Weering J, Brown E, Mantell J, Sharp T, Verkade P (2010) Studying membrane traffic in high resolution. Methods Cell Biol, in press.
16. Verkade P (2008) Moving EM: the rapid transfer system as a new tool for correlative light and electron microscopy and high throughput for high-pressure freezing. J Microsc 230: 317-328.
17. Yarden Y (2001) The EGFR family and its ligands in human cancer: signalling mechanisms and therapeutic opportunities. Eur J Cancer 37: S3-S8.