Multiplex real-time PCR assays for the identification of the potato cyst and tobacco cyst nematodes

Plant Disease

Volumn 94, Issue 8, 2010, Pages 959-965

  Nakhla, Mark K ^a   Owens, Kristina J ^a   Li, Wenbin ^a   Wei, Gang ^a   Skantar, Andrea M ^b   Levy, Laurene ^a  

^a UNITED STATES DEPARTMENT OF AGRICULTURE   (United States)

^b AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

INTERNAL TRANSCRIBED SPACERS; MANAGEMENT PROGRAMS; PALLIDA; QUANTITATIVE PCR; REAL-TIME PCR ASSAY; REAL-TIME POLYMERASE CHAIN REACTION; SPECIFIC PRIMERS;

ASSAYS; MULTIPLEXING; POLYMERASE CHAIN REACTION; TOBACCO;

PROBES;

GLOBODERA; GLOBODERA PALLIDA; GLOBODERA ROSTOCHIENSIS; GLOBODERA TABACUM; NEMATODA; NICOTIANA TABACUM; SOLANUM TUBEROSUM;

EID: 77955634443     PISSN: 01912917     EISSN: None     Source Type: Journal    
DOI: 10.1094/PDIS-94-8-0959     Document Type: Article

References (26)

1. Bačí, J., Stare, B. G., Sčrca, S., and Urek, G. 2008. Analyses of Globodera rostochiensis and G. pallida populations from Serbia by morphometrics and real-time PCR. Russ. J. Nematol. 16:63-65.
2. Baldwin, J. G., and Mundo-Ocampo, M. 1991. Heteroderinae, Cyst- and non-cyst-forming nematodes. Pages 275-362 in: Manual of Agricultural Nematology. W. R. Nickle, ed. Marcel Dekker, New York.
3. Bates, J. A., Taylor, E. J. A., Gans, P. T., and Thomas, J. E. 2002. Determination of relative proportions of Globodera species in mixed populations of potato cyst nematodes using PCR product melting peak analysis. Mol. Plant Pathol. 3:153-161.
4. Bélair, G., and Miller, S. 2006. First report of Globodera tabacum infecting tobacco plants in Quebec, Canada. Plant Dis. 90:527.
5. Bulman, S. R., and Marshall, J. W. 1997. Differentiation of Australasian potato cyst nematode (PCN) populations using the polymerase chain reaction (PCR). N. Z. J. Crop Hortic. 25:123-129.
6. den Nijs, L., and Karssen, G. 2004. Diagnostic protocols for regulated pests, Globodera rostochiensis and Globodera pallida. EPPO Bull. 34:309-314.
7. Hafez, S. L., Sundararaj, P., Handoo, Z. A., Skantar, A. M., Carta, L. K., and Chitwood, D. J. 2007. First report of the pale cyst nematode, Globodera pallida, in the United States. Plant Dis. 91:325.
8. Howlett, B. J., Brownlee, A. G., Guest, D. I., Adcock, G. J., and McFadden, G. I. 1992. The 5S ribosomal RNA gene is linked to large and small subunit ribosomal RNA genes in the oomycetes, Phytophthora vignae, P. cinnamomi, P. megaspera f. sp. glycinae and Saprolegnia ferax. Curr. Genet. 22:455-461.
9. Joyce, S. A., Reid, A., Driver, F., and Curran, J. 1994. Application of polymerase chain reaction (PCR) methods to identification of entomopathogenic nematodes. Pages 178-187 in: COST 812 Biotechnology: Genetics of Entomopathogenic Nematode-Bacterium Complexes. A. M. Burnell, R.-U. Ehlers, and J. P. Masson, eds. Proc. Symp. Workshop, St. Patrick's College, Maynooth, County Kildare, Ireland. European Commission, DG XII, Luxembourg.
10. Li, W., Li, D., Twieg, E., Hartung, J. S., and Levy, L. 2008. Optimized quantification of unculturable Candidatus Liberibacter spp. in host plants using real-time PCR. Plant Dis. 92:854-861.
11. Machay I. M., Ardeb, K. E., and Nitsche, A.2002. Survey and summary/real-time PCR in virology. Nucleic Acids Res. 30:1292-1305.
12. Madani, M., Subbotin, S. A., and Moens, M. 2005. Quantitative detection of the potato cyst nematode, Globodera pallida, and the beet cyst nematode, Heterodera schachtii, using real-time PCR with SYBR green I dye. Mol. Cell Probes 19:81-86.
13. Marché, L., Valette, S., Grenier, E., and Mugniéry, D. 2001. Intra-species DNA polymorphism in the tobacco cyst-nematode complex (Globodera tabacum) using AFLP. Genome 44:941-946.
14. Miller, L. I., and Gray, B. J. 1972. Heterodera solanacearum N. sp., a parasite of solanaceous plants. Nematologica 18:404-413.
15. Mota, M., and Eisenback, J. D. 1993. Morphology of second-stage juveniles and males of Globodera tabacum tabacum, G. t. virginiae, and G. t. solanacearum (Nemata: Heteroderinae). J. Nematol. 25:27-33.
16. Mota, M., and Eisenback, J. D. 1993. Morphology of females and cysts of Globodera tabacum tabacum, G. t. virginiae, and G. t. solanacearum (Nemata: Heteroderinae). J. Nematol. 25:136-147.
17. Mota, M., and Eisenback, J. D. 1993. Morphometrics of Globodera tabacum tabacum, G. t. virginiae, and G. t. solanacearum (Nemata: Heteroderinae). J. Nematol. 25:148-160.
18. Nakhla, M. K., Owens, K. J., Carta, L., Skantar,A., and Levy, L. 2007. Validation of potato cyst nematode (PCN) molecular identification methods and development of laboratory work instructions for a national survey. (Abstr.) Phytopathology 97:S82.
19. Nowaczyk, K., Dobosz, R. Kornobis, S., and Obrepalska-Steplowska, A. 2008. TaqMan REAL-Time PCR-based approach for differentiation between Globodera rostochiensis (golden nematode) and Globodera artemisiae species. Parasitol. Res. 103:577-581.
20. Plantard, O. Picard, D., Valette, S., Scurrah, M, Grenier, E., and Mugniéry, D. 2008. Origin and genetic diversity of Western European populations of the potato cyst nematode (Globodera pallida) inferred from mitochondrial sequences and microsatellite loci. Mol. Ecol. 17:2208-2218.
21. Pylypenko, L. A., Phillips, M. S., and Blok, V. C. 2008. Characterisation of two Ukrainian populations of Globodera pallida in terms of their virulence and mtDNA, and the biological assessment of a new resistant cultivar Vales Everest. Nematology 10:585-590.
22. Quader, M., Nambiar, L., and Cunnington, J. 2008. Conventional and real-time PCR-based species identification and diversity of potato cyst nematodes (Globodera spp.) from Victoria, Australia. Nematology 10:471-478.
23. Skantar, A. M., Handoo, Z. A., Carta, L. K., and Chitwood, D. J. 2007. Morphological and molecular identification of Globodera pallida associated with potato in Idaho. J. Nematol. 39:133-144.
24. Subbotin, S. A., Halford, P. D., Warry, A., and Perry, R. N. 2000. Variations in ribosomal DNA sequences and phylogeny of Globodera parasitizing solanaceous plants. Nematology 2:591-604.
25. Syracuse, A. J., Johnson, C. S., Eisenback, J. D., Nessler, C. L., and Smith, E. P. 2004. Intraspecific variability within Globodera tabacum solanacearum using random amplified polymorphic DNA. J. Nematol. 36:433-439.
26. Thomas, W. K., Vida, J. T., Frisse, L. M., Mundo, M., and Baldwin, J. G. 1997. DNA sequences from formalin fixed nematodes: Integrating molecular and morphological approaches to taxonomy. J. Nematol. 29:250-254.