Alteration of sequence specificity of the type IIS restriction endonuclease Btsi

PLoS ONE

Volumn 5, Issue 7, 2010, Pages

  Guan, Shengxi ^a   Blanchard, Aine ^a   Zhang, Penghua ^a   Zhu, Zhenyu ^a  

^a NEW ENGLAND BIOLABS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENINE; BUFFER; CYTOSINE; GUANINE; THYMINE; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE BTSL; TYPE II SITE SPECIFIC DEOXYRIBONUCLEASE BTSL 1B; UNCLASSIFIED DRUG; RESTRICTION ENDONUCLEASE;

AMINO ACID SEQUENCE; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME SPECIFICITY; MOLECULAR RECOGNITION; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN CLEAVAGE; PROTEIN DOMAIN; WILD TYPE; BACILLUS; DNA SEQUENCE; ENZYME ACTIVE SITE; ENZYMOLOGY; GENETICS; METABOLISM; SITE DIRECTED MUTAGENESIS;

BACILLUS; CATALYTIC DOMAIN; DNA RESTRICTION ENZYMES; MUTAGENESIS, SITE-DIRECTED; SEQUENCE ANALYSIS, DNA;

EID: 77955617577     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0011787     Document Type: Article

References (19)

1. Wilson GG, Murray NE (1991) Restriction and modification systems. Annu Rev Genet 25: 585-627.
2. Roberts RJ, Belfort M, Bestor T, Bhagwat AS, Bickle TA, et al. (2003) A nomenclature for restriction enzymes, DNA methyltransferases, homing endonucleases and their genes. Nucleic Acids Res 31: 1805-1812.
3. Roberts RJ, Vincze T, Posfai J, Macelis D (2010) REBASE-a database for DNA restriction and modification: enzymes, genes and genomes. Nucleic Acids Res 38: D234-236.
4. Xu SY, Zhu Z, Zhang P, Chan SH, Samuelson JC, et al. (2007) Discovery of natural nicking endonucleases Nb.BsrDI and Nb.BtsI and engineering of topstrand nicking variants from BsrDI and BtsI. Nucleic Acids Res 35: 4608-4618.
5. Rimseliene R, Maneliene Z, Lubys A, Janulaitis A (2003) Engineering of restriction endonucleases: using methylation activity of the bifunctional endonuclease Eco57I to select the mutant with a novel sequence specificity. J Mol Biol 327: 383-391.
6. Jurenaite-Urbanaviciene S, Serksnaite J, Kriukiene E, Giedriene J, Venclovas C, et al. (2007) Generation of DNA cleavage specificities of type II restriction endonucleases by reassortment of target recognition domains. Proc Natl Acad Sci USA 104: 10358-10363.
7. Morgan RD, Luyten YA (2009) Rational engineering of type II restriction endonuclease DNA binding and cleavage specificity. Nucleic Acids Res 37: 5222-5233.
8. Zhu Z, Zhou J, Friedman AM, Xu SY (2003) Isolation of BsoBI restriction endonuclease variants with altered substrate specificity. J Mol Biol 330: 359-372.
9. Samuelson JC, Xu SY (2002) Directed evolution of restriction endonuclease BstYI to achieve increased substrate specificity. J Mol Biol 319: 673-683.
10. Joshi HK, Etzkorn C, Chatwell L, Bitinaite J, Horton NC (2006) Alteration of sequence specificity of the type II restriction endonuclease HincII through an indirect readout mechanism. J Biol Chem 281: 23852-23869.
11. Xu Y, Lunnen KD, Kong H (2001) Engineering a nicking endonuclease N.AlwI by domain swapping. Proc Natl Acad Sci USA 98: 12990-12995.
12. Bellamy SR, Milsom SE, Scott DJ, Daniels LE, Wilson GG, et al. (2005) Cleavage of individual DNA strands by the different subunits of the heterodimeric restriction endonuclease BbvCI. J Mol Biol 348: 641-653.
13. Heiter DF, Lunnen KD, Wilson GG (2005) Site-specific DNA-nicking mutants of the heterodimeric restriction endonuclease R.BbvCI. J Mol Biol 348: 631-640.
14. Besnier CE, Kong H (2001) Converting MlyI endonuclease into a nicking enzyme by changing its oligomerization state. EMBO Rep 2: 782-786.
15. Zhang P, Too PH, Samuelson JC, Chan SH, Vincze T, et al. (2010) Engineering BspQI nicking enzymes and application of N.BspQI in DNA labeling and production of single-strand DNA. Protein Expr Purif 69: 226-234.
16. Zhu Z, Samuelson JC, Zhou J, Dore A, Xu SY (2004) Engineering strandspecific DNA nicking enzymes from the type IIS restriction endonucleases BsaI, BsmBI, and BsmAI. J Mol Biol 337: 573-583.
17. Gingeras TR, Milazzo JP, Roberts RJ (1978) A computer assisted method for the determination of restriction enzyme recognifion sites. Nucleic Acids Res 5: 4105-4127.
18. Wei H, Therrien C, Blanchard A, Guan S, Zhu Z (2008) The Fidelity Index provides a systematic quantitation of star activity of DNA restriction endonucleases. Nucleic Acids Res 36: e50.
19. Pingoud A, Jeltsch A (2001) Structure and function of type II restriction endonucleases. Nucleic Acids Res 29: 3705-3727.