Proteolysis approach without chemical modification for a simple and rapid analysis of disulfide bonds using thermostable protease-immobilized microreactors

Proteomics

Volumn 10, Issue 16, 2010, Pages 2942-2949

  Yamaguchi, Hiroshi ^a   Miyazaki, Masaya ^a,b   Maeda, Hideaki ^a,b,c  

^a NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY AIST   (Japan)

^b KYUSHU UNIVERSITY   (Japan)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

Author keywords

Disulfide bond; Enzyme immobilization; Protease; Proteolysis; Technology; Thermal denaturation

Indexed keywords

LYSOZYME; PROTEINASE;

ARTICLE; CHEMICAL MODIFICATION; DISULFIDE BOND; MICROREACTOR; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DEGRADATION; PROTEIN PURIFICATION; PROTEIN STABILITY; REACTOR; THERMOSTABILITY;

ANIMALS; BIOREACTORS; BIOTECHNOLOGY; CATTLE; DISULFIDES; ENZYME STABILITY; ENZYMES, IMMOBILIZED; HIGH-THROUGHPUT SCREENING ASSAYS; MURAMIDASE; PEPTIDE HYDROLASES; PROTEIN DENATURATION; SERUM ALBUMIN, BOVINE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TEMPERATURE;

EID: 77955598985     PISSN: 16159853     EISSN: 16159861     Source Type: Journal    
DOI: 10.1002/pmic.201000166     Document Type: Article

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