Validation of a nylon-flocked-swab protocol for efficient recovery of bacterial spores from smooth and rough surfaces

Applied and Environmental Microbiology

Volumn 76, Issue 15, 2010, Pages 5148-5158

  Probst, Alexander ^a   Facius, Rainer ^b   Wirth, Reinhard ^a   Moissl Eichinger, Christine ^a  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b GERMAN AEROSPACE CENTER DLR   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ANTHRACIS; BACILLUS ANTHRACIS STERNE; BACILLUS ATROPHAEUS; BACILLUS SPECIES; BACTERIAL SPORE; LIFE DETECTION; LOAD DETERMINATION; MICROBIAL CONTAMINATION; NATIONAL AERONAUTICS AND SPACE ADMINISTRATIONS; PROTECTION REQUIREMENTS; RECOVERY EFFICIENCY; RECOVERY RATE; ROUGH SURFACES; SCANNING ELECTRON MICROGRAPHS; STANDARD METHOD; STANDARD PROTOCOLS;

BACTERIOLOGY; INTERPLANETARY SPACECRAFT; NASA; POLLUTION DETECTION; POLYAMIDES; RAYON; SCANNING ELECTRON MICROSCOPY; STAINLESS STEEL;

RECOVERY;

NYLON;

BACTERIUM; EXPERIMENTAL STUDY; INOCULATION; LAW ENFORCEMENT; SCANNING ELECTRON MICROSCOPY; SECURITY; SPACECRAFT; SPORE; STEEL; SURFACE ROUGHNESS;

ARTICLE; BACILLUS; BACTERIAL SPORE; COMPARATIVE STUDY; EVALUATION; GOVERNMENT; ISOLATION AND PURIFICATION; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; SENSITIVITY AND SPECIFICITY; SPACE FLIGHT; UNITED STATES; EVALUATION STUDY; PROCEDURES;

BACILLUS; BACTERIOLOGICAL TECHNIQUES; ENVIRONMENTAL MICROBIOLOGY; NYLONS; SENSITIVITY AND SPECIFICITY; SPACECRAFT; SPORES, BACTERIAL; UNITED STATES; UNITED STATES NATIONAL AERONAUTICS AND SPACE ADMINISTRATION;

BACILLUS (BACTERIUM); BACILLUS ANTHRACIS; BACILLUS ANTHRACIS STR. STERNE; BACILLUS ATROPHAEUS; BACTERIA (MICROORGANISMS); GOSSYPIUM HIRSUTUM;

EID: 77955585150     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00399-10     Document Type: Article

References (40)

1. Abu-Diab, A., M. Azzeh, R. Ghneim, M. Zoughbi, S. Turkuman, N. Rishmawi, A. E. Issa, I. Siriani, R. Dauodi, R. Kattan, and M. Y. Hindiyeh. 2008. Comparison between pemasal flocked swabs and nasopharyngeal aspirates for detection of common respiratory viruses in samples from children. J. Clin. Microbiol. 46:2414-2417.
2. Angelotti, R., M. J. Foter, K. A. Busch, and K. H. Lewis. 1958. A comparative evaluation of methods for determining the bacterial contamination on surfaces. Food Res. 23:175-185.
3. Anonymous. 2003. USP 1223: validation of alternative microbiological methods. Pharm. Forum 29:256-264.
4. Brown, G. S., R. G. Betty, J. E. Brockmann, D. A. Lucero, C. A. Souza, K. S. Walsh, R. M. Boucher, M. Tezak, M. C. Wilson, and T. Rudolph. 2007. Evaluation of a wipe surface sample method for collection of Bacillus spores from nonporous surfaces. Appl. Environ. Microbiol. 73:706-710.
5. Brown, G. S., R. G. Betty, J. E. Brockmann, D. A. Lucero, C. A. Souza, K. S. Walsh, R. M. Boucher, M. S. Tezak, and M. C. Wilson. 2007. Evaluation of vacuum Alter sock surface sample collection method for Bacillus spores from porous and non-porous surfaces. J. Environ. Monit. 9:666-671.
6. Brown, G. S., R. G. Betty, J. E. Brockmann, D. A. Lucero, C. A. Souza, K. S. Walsh, R. M. Boucher, M. S. Tezak, M. C. Wilson, T. Rudolph, H. D. Lindquist, and K. F. Martinez. 2007. Evaluation of rayon swab surface sample collection method for Bacillus spores from nonporous surfaces. J. Appl. Microbiol. 103:1074-1080.
7. Bruckner, J., and K. Venkateswaran. 2007. Overview of methodologies to sample and assess microbial burden in low biomass environment. Jpn. J. Food Microbiol. 24:61-70.
8. Buttner, M. P., P. Cruz-Perez, and L. D. Stetzenbach. 2001. Enhanced detection of surface-associated bacteria in indoor environments by quantitative PCR. Appl. Environ. Microbiol. 67:2564-2570.
9. Buttner, M. P., P. Cruz, L. D. Stetzenbach, A. K. Klima-Comba, V. L. Stevens, and P. A. Emanuel. 2004. Evaluation of the Biological Sampling Kit (BiSKit) for large-area surface sampling. Appl. Environ. Microbiol. 70:7040-7045.
10. Chernesky, M., S. Castriciano, D. Jang, and M. Smieja. 2006. Use of flocked swabs and a universal transport medium to enhance molecular detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J. Clin. Microbiol. 44: 1084-1086.
11. Commitee on Space Research. 2005. COSFAR planetary protection policy. COSPAR/IAU Workshop on Planetary Protection, October 2002 ed., amended 2005. Commitee on Space Research (COSPAR), International Council for Science, Paris, France. http://cosparhq.cnes.fr/Scistr/Pppolicy .htm.
12. Dalmaso, G., M. Bini, R. Paroni, and M. Ferrari. 2008. Qualifiction of high-recovery, flocked swabs as compared to traditional rayon swabs for microbiological environmental monitoring of surfaces. J. Pharm. Sci. Technol. 63:191-199.
13. Edmonds, J. M., P. J. Collett, E. R. Valdes, E. W. Skowronski, G. J. Pellar, and P. A. Emanuel. 2009. Surface sampling of spores in dry-deposition aerosols. Appl. Environ. Microbiol. 75:39-44.
14. Estill, C. F., P. A. Baron, J. K. Beard, M. J. Hein, L. D. Larsen, L. Rose, F. W. Schaefer III, J. Noble-Wang, L. Hodges, H. D. Lindquist, G. J. Deye, and M. J. Arduino. 2009. Recovery efficiency and limit of detection of aerosolized Bacillus anthracis Sterne from environmental surface samples. Appl. Environ. Microbiol. 75:4297-4306.
15. European Cooperation for Space Standardization. 2008. Microbial examination of flight hardware and cleanrooms. ECSS-Q.-ST-70-55C. European Cooperation for Space Standardization (ESA-ESTEC), Noordwijk, Netherlands.
16. Fast, P. G. 1972. The σ-endotoxin of Bacillus thuringiensis III: a rapid method for separating parasporal bodies from spores. J. Invertebr. Pathol. 20:139-140.
17. Hachisuka, Y., K. Kojima, and T. Sato. 1966. Fine filaments on the outside of the exosporium of Bacillus anthracis spores. J. Bacteriol. 91:2382-2384.
18. Hodges, L. R., L. J. Rose, A. Peterson, J. Noble-Wang, and M. J. Arduino. 2006. Evaluation of a macrofoam swab protocol for the recovery of Bacillus anthracis spores from a steel surface. Appl. Environ. Microbiol. 72:4429-4430.
19. Kirschner, L. E., and J. R. Puleo. 1979. Wipe-rinse technique for quantitating microbial contamination on large surfaces. Appl. Environ. Microbiol. 38:466-470.
20. Krech, T., S. Castriciano, D. Jang, M. Smieja, G. Enders, and M. Chernesky. 2009. Detection of high risk HPV and Chlamydia trachomatis in vaginal and cervical samples collected with flocked nylon and wrapped rayon dual swabs transported in dry tubes. J. Virol. Methods 162:291-293.
21. La Duc, M. T., A. Dekas, S. Osman, C. Moissl, D. Newcombe, and K. Venkateswaran. 2007. Isolation and characterization of bacteria capable of tolerating the extreme conditions of clean room environments. Appl. Environ. Microbiol. 73:2600-2611.
22. La Duc, M. T., R. Kern, and K. Venkateswaran. 2004. Microbial monitoring of spacecraft and associated environments. Microb. Ecol. 47:150-158.
23. La Duc, M. T., S. Osman, P. Vaishampayan, Y. Piceno, G. Andersen, J. A. Spry, and K. Venkateswaran. 2009. Comprehensive census of bacteria in clean rooms by using DNA microarray and cloning methods. Appl. Environ. Microbiol. 75:6559-6567.
24. Moeller, R., G. Horneck, R. Facius, and E. Stackebrandt. 2005. Role of pigmentation in protecting Bacillus sp. endospores against environmental UV radiation. FEMS Microbiol. Ecol. 51:231-236.
25. Moissl, C., S. Osman, M. T. La Duc, A. Dekas, E. Brodie, T. DeSantis, and K. Venkateswaran. 2007. Molecular bacterial community analysis of clean rooms where spacecraft are assembled. FEMS Microbiol. Ecol. 61:509-521.
26. National Aeronautics and Space Administration. 1999. NASA standard procedures for the microbiological examination of space hardware, NPG 5340.1D. Jet Propulsion Laboratory Communication. National Aeronautics and Space Administration, Pasadena, CA.
27. Pfanzagl, J., and F. Puntigam. 1961. Aussagen über den Quotienten zweier Poisson-Parameter und deren Anwendungen auf ein Problem der Pockenschutzimpfung. Biom. Z. 3:135-142.
28. Probst, A., P. Vaishampayan, S. Osman, C. Moissl-Eichinger, G. L. Andersen, and K. Venkateswaran. 2010. Diversity of anaerobic microbes in spacecraft assembly clean rooms. Appl. Environ. Microbiol. 76:2837-2845.
29. Przyborowski, J., and H. Wilenski. 1940. Homogeneity of results in testing samples from Poisson series. Biometrika 31:313-323.
30. Puleo, J. R., N. D. Fields, S. L. Bergstrom, G. S. Oxborrow, P. D. Stabekis, and R. Koukol. 1977. Microbiological profiles of the Viking spacecraft. Appl. Environ. Microbiol. 33:379-384.
31. Rönner, U., U. Husmark, and A. Henriksson. 1990. Adhesion of Bacillus spores in relation to hydrophobicity. J. Appl. Bacteriol. 69:550-556.
32. Rose, L., B. Jensen, A. Peterson, S. N. Banerjee, and M. J. Srduino. 2004. Swab materials and Bacillus anthracis spore recovery from nonporous surfaces. Emerg. Infect. Dis. 10:1023-1029.
33. Sahai, H., and S. C. Misra. 1992. Comparing means of two Poisson distributions. Math. Sci. 17:60-67.
34. Schuerger, A. C., R. L. Mancinelli, R. G. Kern, L. J. Rothschild, and C. P. McKay. 2003. Suivival of endospores of Bacillus subtilis on spacecraft surfaces under simulated Martian environments: implications for the forward contamination of Mars. Icarus 165:253-276.
35. Schuerger, A. C., J. T. Richards, P. E. Hintze, and R. G. Kern. 2005. Surface characteristics of spacecraft components affect the aggregation of microorganisms and may lead to different survival rates of bacteria on Mars landers. Astrobiology 5:545-559.
36. Schuerger, A. C., S. Trigwell, and C. I. Calle. 2008. Use of non-thermal atmospheric plasmas to reduce the viability of Bacillus subtilis on spacecraft surfaces. Int. J. Astrobiol. 7:47-57.
37. 2O at the Phoenix landing site. Science 325:58-61.
38. Stieglmeier, M., R. Wirth, G. Kminek, and C. Moissl-Eichinger. 2009. Cultivation of anaerobic and facultatively anaerobic bacteria from spacecraftassociated clean rooms. Appl. Environ. Microbiol. 75:3484-3491.
39. Strong, D. H., M. J. Woodburn, and M. M. Mancini. 1961. Preliminary observations on the effect of sodium alginate on selected nonsporing organisms. Appl. Microbiol. 9:213-218.
40. Venkateswaran, K., M. Satomi, S. Chung, R. Kern, R. Koukol, C. Basic, and D. White. 2001. Molecular microbial diversity of a spacecraft assembly facility. Syst. Appl. Microbiol. 24:311-320.