Evaluation of the limulus amebocyte lysate and recombinant factor C assays for assessment of airborne endotoxins

Applied and Environmental Microbiology

Volumn 76, Issue 15, 2010, Pages 4988-4995

  Thorne, Peter S ^a   Perry, Sarah S ^a   Saito, Rena ^b   O'Shaughnessy, Patrick T ^a   Mehafly, John ^b   Metwali, Nervana ^a   Keefe, Thomas ^b   Donham, Kelley J ^a   Reynolds, Stephen J ^b  

^a UNIVERSITY OF IOWA   (United States)

^b Department of Environmental and Radiological Health Sciences   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AIR SAMPLES; AIR SAMPLING; AIRBORNE DUSTS; AIRBORNE ENDOTOXIN; ANALYTES; DAIRY COW; DOMESTIC ENVIRONMENTS; EXPOSURE ASSESSMENT; EXPOSURE CHAMBERS; FIELD SAMPLING; FIELD STUDIES; FLUOROMETRIC ASSAY; FREE WATER; GEOMETRIC MEAN; INSTITUTE OF OCCUPATIONAL MEDICINES; LIMULUS AMEBOCYTE LYSATE; LIVESTOCK PRODUCTION; LOWER LIMITS OF DETECTIONS; MIXED-MODEL ANALYSIS; ORGANIC DUSTS; PAIRED SAMPLE; TWEEN-20;

AGRICULTURE; AIR QUALITY; DUST; EXTRACTION; GENE TRANSFER; REGRESSION ANALYSIS;

ASSAYS;

ENDOTOXIN; ENZYME PRECURSOR; FACTOR C; SERINE PROTEINASE; ARTHROPOD PROTEIN; LIMULUS CLOTTING FACTOR C;

AIR SAMPLING; BIOASSAY; CORRELATION; ENZYME ACTIVITY; INVERTEBRATE; LABORATORY METHOD; RECOMBINATION; TOXIN;

AIR; ANIMAL; ANIMAL HOUSING; ARTICLE; COMPARATIVE STUDY; DOMESTIC ANIMAL; EVALUATION; LIMULUS LYSATE TEST; METABOLISM; METHODOLOGY; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; SENSITIVITY AND SPECIFICITY; ANALYSIS; EVALUATION STUDY; PROCEDURES;

AIR; AIR MICROBIOLOGY; ANIMALS; ANIMALS, DOMESTIC; BACTERIOLOGICAL TECHNIQUES; ENDOTOXINS; ENZYME PRECURSORS; HOUSING, ANIMAL; LIMULUS TEST; SENSITIVITY AND SPECIFICITY; SERINE ENDOPEPTIDASES; ARTHROPOD PROTEINS;

BOS; EQUIDAE; LIMULUS; SUIDAE;

EID: 77955568533     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00527-10     Document Type: Article

References (38)

1. Alwis, K. U., and D. K. Milton. 2006. Recombinant factor C assay for measuring endotoxin in house dust: comparison with LAL, and (l-3)-β- Dglucans. Am. J. Ind. Med. 49:296-300.
2. Braun-Fahrländer, C., J. Riedler, U. Herz, W. Eder, M. Waser, L. Grize, S. Maisch, D. Carr, F. Gerlach, A. Bufe, R. P. Lauener, R. Schierl, H. Renz, D. Nowak, E. von Mutius, and the Allergy and Endotoxin Study Team. 2002. Environmental exposure to endotoxin and its relation to asthma in schoolage children. N. Engl. J. Med. 347:869-877.
3. Cooper, J. F. 1990. Resolving LAL test interferences. J. Parent. Sci. Technol. 44:13-15.
4. Ding, J. L., M. A. Navas III, and B. Ho. 1995. Molecular cloning and sequence analysis of factor C cDNA from the Singapore horseshoe crab, Carcinoscorpius rotundicauda. Mol. Mar. Biol. Biotechnol. 4:90-103.
5. Donham, K. J., D. Cumro, S. J. Reynolds, and J. A. Merchant. 2000. Doseresponse relationships between occupational aerosol exposures and crossshift declines of lung function in poultry workers: recommendations for exposure limits. J. Occup. Environ. Med. 42:260-269.
6. Douwes, J., P. S. Thorne, N. Pearce, and D. Heederik. 2003. Bioaerosol health effects and exposure assessment: progress and prospects. Ann. Occup. Hyg. 47:187-200.
7. Douwes, J., P. Versloot, A. Hollander, D. Heederik, and G. Doekes. 1995. Influence of various dust sampling and extraction methods on the measurement of airborne endotoxin. Appl. Environ. Microbiol. 61:1763-1769.
8. Gehring, U., G. Bolte, M. Borte, D. Heederik, and G. Doekes. 2001. Exposure to endotoxin decreases the risk of atopic eczema in infancy: a cohort study. J. Allergy Clin. Immunol. 108:847-854.
9. Gereda, J. E., D. Y. M. Leung, A. Thatayatikom, J. E. Streib, M. R. Price, M. D. Klinnert, and A. H. Liu. 2000. Relation between house-dust endotoxin exposure, type 1 T-cell development, and allergen sensitisation in infants at high risk of asthma. Lancet 355:1680-1683.
10. Hadina, S., J. P. Weiss, P. B. McCray Jr., K. Kulhankova, and P. S. Thorne. 2008. MD-2-dependent pulmonary immune responses to inhaled lipopolysaccharides. Am. J. Respir. Cell Mol Biol. 38:647-654.
11. Hinds, W. C. 1980. Dry-dispersion aerosol generators, p. 171-187. In K. Willeke (ed.), Generation of aerosols and facilities for exposure experiments. Ann Arbor Science Publishers, Inc., Ann Arbor, MI.
12. Hollander, A, D. Heederik, P. Versloot, and J. Douwes. 1993. Inhibition and enhancement in the analysis of airborne endotoxin levels in various occupational environments. Am. Ind. Hyg. Assoc. J. 54:647-653.
13. Lee, J. A., J. C. Johnson, S. J. Reynolds, P. S. Thorne, and P. T. O'Shaughnessy. 2006. Indoor and outdoor air quality assessment of four wastewater treatment plants. J. Occup. Environ. Hyg. 3:36-43.
14. Milton, D. K., R. J. Gere, H. A. Feldman, and I. A. Greaves. 1990. Endotoxin measurement: aerosol sampling and application of a new Limulus method. Am. Ind. Hyg. Assoc. J. 51:331-337.
15. Milton, D. K., D. K. Johnson, and J.-H. Park. 1997. Environmental endotoxin measurement: interference and sources of variation in the Limulus assay for house dust. Am. Ind. Hyg. Assoc. J. 58:861-867.
16. O'Shaughnessy, P. T., J. Lo, V. Golla, J. Nakatsu, M. I. Tillery, and S. J. Reynolds. 2007. Comparison of aerosol samplers relative to the inhalable and respirable collection criteria. J. Occup. Environ. Hyg. 4:237-245.
17. Portengen, L., L. Preller, M. Tielen, G. Doekes, and D. Heederik. 2005. Endotoxin exposure and atopic sensitization in adult pig farmers. J. Allergy Clin. Immunol. 115:797-802.
18. Reynolds, S. J., K. J. Donham, P. Whitten, J. A. Merchant, L. F. Burmeister, and W. J. Popendorf. 1996. Longitudinal evaluation of dose-response relationships for environmental exposures and pulmonary function in swine production workers. Am. J. Ind. Med. 29:33-40.
19. Reynolds, S. J., D. K. Milton, D. Heederik, P. S. Thorne, K. J. Donham, E. A. Croteau, K. M. Kelly, J. Douwes, D. Lewis, M. Whitmer, I. Connaughton, S. Koch, P. Malmberg, B. M. Larsson, J. Deddens, A. Saraf, and L. Larsson. 2005. Interlaboratory evaluation of endotoxin analyses in agricultural dust- comparison of LAL assay and mass spectrometry. J. Environ. Monit. 7:13711377.
20. Reynolds, S. J., J. Nakatsu, M. I. Tillery, T. Keefe, J. Mehafty, P. S. Thorne, K. J. Donham, M. Nonnenmann, V. Golla, and P. T. O'Shaughnessy. 2009. Field and wind tunnel comparison of four aerosol samplers using agricultural dusts. Ann. Occup. Hyg. 53:585-594.
21. Reynolds, S. J., P. S. Thorne, K. J. Donham, E. A. Croteau, K. M. Kelly, D. Lewis, M. Whitmer, D. J. Heederik, J. Douwes, I. Connaughton, S. Koch, P. Malmberg, B. M. Larsson, and D. K. Milton. 2002. Comparison of endotoxin assays using agricultural dusts. Am. Ind. Hyg. Assoc. J. 63:430-438.
22. Roslansky, P. F., and T. J. Novitsky. 1991. Sensitivity of Limulus amebocyte lysate to LAL-reactive glucans. J. Clin. Microbiol. 29:2477-2483.
23. Saito, R., B. K. Cranmer, J. D. Tessari, L. Larsson, J. M. Mehaffy, T. J. Keefe, and S. J. Reynolds. 2009. Recombinant Factor C (rFC) assay and gas chromatography/mass spectrometry (GC/M.S) analysis of endotoxins in four agricultural dusts. Ann. Occup. Hyg. 53:713-722.
24. Schwartz, D. A., P. S. Thorne, S. J. Yagla, L. F. Burmeister, S. A. Olenchock, J. L. Watt, and T. J. Quinn. 1995. The role of endotoxin in grain dustinduced lung disease. Am. J. Respir. Crit. Care Med. 152:603-608.
25. Smid, T., D. Heederik, R. Houba, and P. H. Quamjer. 1994. Dust- and endotoxin-related acute lung function changes and work-related symptoms in workers in the animal feed industry. Am. J. Ind. Med. 25:877-888.
26. Smit, L. A., D. Heederik, G. Doekes, C. Blom, I. van Zweden, and I. M. Wouters. 2008. Exposure-response analysis of ailergy and respiratory symptoms in endotoxin-exposed adults. Eur. Respir. J. 31:1241-1248.
27. Spaan, S., I. M. Wouters, I. Oosting, G. Doekes, and D. Heederik. 2006. Exposure to inhalable dust and endotoxin in agricultural industries. J. Environ. Monit. 8:63-72.
28. Spaan, S., L. A. M. Smit, W. Eduard, L. Larsson, H. J. Arts, I. M. Wouters, and D. J. Heederik. 2008. Endotoxin exposure in sewage treatment workers: investigation of exposure variability and comparison of analytical techniques. Ann. Agric. Environ. Med. 15:251-261.
29. Spaan, S., G. Doekes, D. Heederik, P. S. Thorne, and I. M. Wouters. 2008. Effect of extraction and assay media on analysis of airborne endotoxin. Appl. Environ. Microbiol. 74:3804-3811.
30. Spaan, S., D. J. Heederik, P. S. Thorne, and I. M. Wouters. 2007. Optimization of airborne endotoxin exposure assessment: effects of filter type, transport conditions, extraction solutions, and storage of samples and extracts. Appl. Environ. Microbiol. 73:6134-6143.
31. Thorne, P. S. 2000. Inhalation toxicology models of endotoxin and bioaerosol-induced inflammation. Toxicology 152:13-23.
32. Thorne, P. S., R. D. Cohn, D. Mav, S. J. Arbes, Jr., and D. C. Zeldin. 2009. Predictors of endotoxin levels in U.S. housing. Environ. Health Perspect. 117:763-771.
33. Thorne, P. S., K. Kulhankova, M. Yin, R. Cohn, S. J. Arbes Jr., and D. C. Zeldin. 2005. Endotoxin exposure is a risk factor for asthma. Am. J. Respir. Crit. Care Med. 172:1371-1377.
34. Thorne, P. S., S. J. Reynolds, D. K. Milton, P. D. Bloebaum, X. Zhang, P. Whitten, and L. F. Burmeister. 1997. Field evaluation of endotoxin air sampling assay methods. Am. Ind. Hyg. Assoc. J. 58:792-799.
35. Vogelzang, P. F. J., J. W. J. van der Gulden, H. Folgering, D. Heederik, M. J. Tielen, and C. P. van Schayck. 2000. Longitudinal changes in bronchial responsiveness associated with swine confinement dust exposure. Chest 117: 1488-1495.
36. Vogelzang, P. F. J., J. W. J. van der Gulden, H. Folgering, J. J. Kolk, D. Heederik, L. Preller, M. J. Tielen, and C. P. van Schayck. 1998. Endotoxin exposure as a major determinant of lung function decline in pig farmers. Am. J. Respir. Crit. Care Med. 157:15-18.
37. Wouters, I. M., S. Spaan, J. Douwes, G. Doekes, and D. Heederik. 2006. Overview of personal occupational exposure levels to inhalable dust, endotoxin, beta (1→3)-glucan and fungal extracellular polysaccharides in the waste management chain. Ann. Occup. Hyg. 50:39-53.
38. Zhiping, W., P. Malmberg, B. M. Larsson, K. Larsson, L. Larsson, and A. Saraf. 1996. Exposure to bacteria in swine-house dust and acute inflammatory reactions in humans. Am. J. Respir. Crit. Care Med. 154:1261-1266.