Flotation as a tool for indirect DNA extraction from soil

Applied Microbiology and Biotechnology

Volumn 87, Issue 5, 2010, Pages 1927-1933

  Parachin, Nádia Skorupa ^a   Schelin, Jenny ^a   Norling, Börje ^b   Rådström, Peter ^a   Gorwa Grauslund, Marie F ^a  

^a LUND INSTITUTE OF TECHNOLOGY   (Sweden)

^b Quintessence Research AB   (Sweden)

Author keywords

DNA extraction; Humic acid and flotation; Soil

Indexed keywords

BUOYANT DENSITY; CELL LYSIS; COLLOIDAL SOLUTIONS; DENSITY GRADIENTS; DIRECT EXTRACTION; DNA EXTRACTION; DNA LIGATION; DNA YIELDS; DOWNSTREAM APPLICATIONS; EUKARYOTIC SEQUENCES; FURTHER DEVELOPMENT; HIGH MOLECULAR WEIGHT; HUMIC ACID; HUMIC ACID AND FLOTATION; MICROBIAL CELLS; MOLECULAR LEVELS; RESTRICTION ENZYMES; SAMPLE PREPARATION; SAMPLE PREPARATION METHODS; SILICA PARTICLES; SOIL MATRICES; SOIL MICRO-ORGANISMS; SOIL PARTICLES; SPECIFIC PRIMERS;

DNA; ENZYMES; FLOTATION; ORGANIC ACIDS; SILICA; SOILS;

GENES;

RESTRICTION ENDONUCLEASE; RIBOSOME RNA; SILICON DIOXIDE; DNA; SOIL;

COLLOID; DNA; ENZYME ACTIVITY; EXTRACTION METHOD; HUMIC ACID; INHIBITION; INHIBITOR; LYSIS; MOLECULAR ANALYSIS; POLYMERASE CHAIN REACTION; POPULATION DENSITY; SILICA; SOIL MICROORGANISM;

ARTICLE; CONTROLLED STUDY; CYTOLYSIS; DNA EXTRACTION; ENZYME INHIBITION; EUKARYOTE; FLOTATION; POLYMERASE CHAIN REACTION; PROKARYOTE; SOIL ANALYSIS; SOIL MICROFLORA; ANALYSIS; DENSITY GRADIENT CENTRIFUGATION; FRACTIONATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METAGENOMICS; PROCEDURES; SOIL;

CENTRIFUGATION, DENSITY GRADIENT; CHEMICAL FRACTIONATION; DNA; DNA RESTRICTION ENZYMES; METAGENOMICS; POLYMERASE CHAIN REACTION; SOIL;

EUKARYOTA; PROKARYOTA;

EID: 77955558634     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2691-3     Document Type: Article

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