Functional expression of a thermophilic glucuronoyl esterase from Sporotrichum thermophile: Identification of the nucleophilic serine

Applied Microbiology and Biotechnology

Volumn 87, Issue 5, 2010, Pages 1765-1772

  Topakas, Evangelos ^a   Moukouli, Maria ^a   Dimarogona, Maria ^a   Vafiadi, Christina ^a   Christakopoulos, Paul ^a  

^a NATIONAL TECHNICAL UNIVERSITY OF ATHENS   (Greece)

Author keywords

Active site; Glucuronoyl esterase; Lignin carbohydrate complex; Nucleophilic serine; Pichia pastoris; Sporotrichum thermophile

Indexed keywords

ACTIVE SITE; GLUCURONOYL; LIGNIN-CARBOHYDRATE COMPLEX; PICHIA PASTORIS; SPOROTRICHUM THERMOPHILE;

CARBOHYDRATES; CLONING; ENZYME ACTIVITY; ENZYMES; ESTERS; GENES; GLUCOSE; LIGNIN; PHOSPHATASES; YEAST;

AMINO ACIDS;

ESTERASE; FUNGAL ENZYME; GLUCURONOYLESTERASE; UNCLASSIFIED DRUG; FUNGAL PROTEIN; GLUCURONIC ACID; MUTANT PROTEIN; SERINE;

CARBOHYDRATE; CATALYSIS; ENZYME ACTIVITY; FUNGUS; GENE EXPRESSION; GENETIC ANALYSIS; GENETIC ENGINEERING; HOMOLOGY; LIGNIN; MUTATION; PH;

ARTICLE; ENZYME ACTIVITY; GENE IDENTIFICATION; MOLECULAR CLONING; NONHUMAN; NUCLEOPHILICITY; NUCLEOTIDE SEQUENCE; PH MEASUREMENT; PROTEIN EXPRESSION; SPOROTHRIX; SPOROTRICHUM THERMOPHILE; TEMPERATURE SENSITIVITY; AMINO ACID SEQUENCE; CHEMISTRY; CONSENSUS SEQUENCE; ENZYME ACTIVE SITE; ENZYME STABILITY; ENZYMOLOGY; GENETICS; METABOLISM; PH; PICHIA; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY; SITE DIRECTED MUTAGENESIS; TEMPERATURE;

AMINO ACID SEQUENCE; CATALYTIC DOMAIN; CLONING, MOLECULAR; CONSENSUS SEQUENCE; ENZYME STABILITY; ESTERASES; FUNGAL PROTEINS; GLUCURONATES; HYDROGEN-ION CONCENTRATION; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; PICHIA; SEQUENCE ALIGNMENT; SEQUENCE HOMOLOGY, AMINO ACID; SERINE; SPOROTHRIX; TEMPERATURE;

CORYNASCUS HETEROTHALLICUS; FUNGI; PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 77955550027     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2655-7     Document Type: Article

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