Comparison of thin-layer chromatography, spectrofluorimetry and bright greenish-yellow fluorescence test for aflatoxin detection in corn

Brazilian Archives of Biology and Technology

Volumn 53, Issue 3, 2010, Pages 687-692

  Ono, Elisabete Yurie Sataque ^a   da Silva, Marcelo ^a   Ribeiro, Ricardo Marcelo Reche ^b   Ono, Mario Augusto ^a   Hayashi, Luciana ^b   Garcia, Glauco Tironi ^c   Hirooka, Elisa Yoko ^b  

^a STATE UNIVERSITY OF LONDRINA   (Brazil)

^b Departamento de Ciência e Tecnologia de Alimentos   (Brazil)

^c Integrada Cooperativa Agroindustrial ^*  (Brazil)

Author keywords

Aflatoxins; BGYF; Corn; Mycotoxins; Spectrofluorimetry; Thin layer chromatography

Indexed keywords

ANIMALIA; ASPERGILLUS; ZEA MAYS;

EID: 77955539909     PISSN: 15168913     EISSN: 15168913     Source Type: Journal    
DOI: 10.1590/S1516-89132010000300025     Document Type: Article

References (21)

1. Agência Nacional De Vigilância Sanitária (ANVISA), Brasil, 2002. Regulamento técnico sobre limites máximos de aflatoxinas admissíveis no leite, no amendoim, no milho, constante do anexo desta Resolução. Resolução RDC n°274. Available from: Accessed 2006 September 17.
2. Barna-Vetró, I.; Solti, L.; Téren, J.; Gyöngyösi, A.; Szabó, E. and Wölfling A. (1996), Sensitive ELISA test for determination of ochratoxin A. Journal of Agricultural and Food Chemistry, 44, 4071-4074.
3. Beaver, R.W.; Wilson, D.M. and Trucksess, M.W. (1990), Comparison of postcolumn derivatization liquid chromatography with thin-layer chromatography for determination of aflatoxins in naturally contaminated corn. Journal of the Association of Official Analytical Chemists, 73, 579-581.
4. BRASIL. Portaria n.845 de 8 de novembro de 1976 Diário Oficial da República Federativa do Brasil. Brasília, 30 de novembro de 1976.
5. Gimeno, A. (1979), Thin-layer chromatographic determination of aflatoxins, ochratoxins, sterigmatocystin, zearalenone, citrinin, T-2 toxin, diacetoxyscirpenol, penicillic acid, patulin and penitrem. Journal of the Association of Analytical Chemists, 62, 579-585.
6. Glória, E. M.; Fonseca, H. and Souza, I. M. (1998), Ocurrence of mycotoxins in maize delivered to the food industry in Brazil. Food Additives and Contaminants, 15, 181-184.
7. Hussein, H. S. and Brasel, J. M. (2001), Review: Toxicity, metabolism and impact of mycotoxins on humans and animals. Toxicology, 167, 101-134.
8. Marsh, P. B.; Simpson, M. E.; Ferretti, R. J.; Merola, G. V.; Donoso, J.; Craig, G. O.; Trucksess, M. W. and Work, P. S. (1969), Mechanism of formation of a fluorescence in cotton fiber associated with aflatoxin in the seeds at harvest. Journal of Agricultural and Food Chemistry, 17, 468-472.
9. Nilüfer, D. and Boyacioglu, D. (2002), Comparative study of three different methods for the determination of aflatoxins in tahini. Journal of Agricultural and Food Chemistry, 50, 3375-3379.
10. Ono, E. Y. S.; Biazon, L.; Silva, M.; Vizoni, E.; Sugiura, Y.; Ueno, Y. and Hirooka, E. Y. (2006), Fumonisins in corn: Correlation with Fusarium sp. count, damaged kernels, protein and lipid content. Braz. Arch. Biol. Technol., 49, 63 - 71.
11. Palomino, M.E.T.; Fonseca, H.; Glória, E.M.; Calori-Domingues, M.A. and Marques, C. (1998), Avaliação do método de triagem para análise de milho contaminado com aflatoxinas pela fluorescência amarelo-esverdeada brilhante (BGYF- bright greenish yellow fluorescence). Scientia agrícola, 55, 503-508.
12. Patey, A.L.; Sharman, M. and Gilbert, J. (1991), Liquid chromatographic determination of aflatoxin levels in peanut butters using an immunoaffinity column cleanup method:International collaborative trial. Journal of the Association of Official Analytical Chemists, 74, 76-81.
13. Pestka, J.J.; Abouzied, M.N. and Sutikno. (1995), Immunological assays for mycotoxin detection. Food Technology, 2, 120-128.
14. Shotwell, O. L. and Hesseltine, C. W. (1981), Use of bright greenish yellow fluorescence as presumptive test for aflatoxin in corn. Cereal Chemistry. 58, 124-127.
15. Shotwell, O. L.; Goulden, M. L. and Hesseltine, C. W. (1974), Aflatoxin: distribution in contaminated corn. Cereal Chemistry, 51, 492-499.
16. Soares, L. M. V. and Rodriguez-Amaya, D. B. (1989), Survey of aflatoxins, ochratoxins, zearalenone and sterigmatocystin in some Brazilian foods by using multi-toxin thin layer chromatographic method. Journal of the Association of Official Analytical Chemists International, 72, 22-26.
17. Sydenham, E.W.; Shephard, G.S.; Thiel, P.G.; Bird, C. and Miller, B.M. (1996a), Determination of fumonisins in corn: Evaluation of competitive immunoassay and HPLC techniques. Journal of Agricultural and Food Chemistry, 44, 159-164.
18. Sydenham, E.W.; Stockenstrom, S.; Thiel, P.G.; Rheeder, J.P.; Doko, M.B.; Bird, C. and Miller, B.M. (1996b), Polyclonal antibody-based ELISA and HPLC methods for the determination of fumonisins in corn: A comparative study. Journal of Food Protection, 59, 893- 897.
19. Thompson, C. and Henke, S. E. (2000), Effect of climate and type of storage container on aflatoxin production in corn and its associated risks to wildlife species. Journal of Wildlife Diseases, 36, 172-179.
20. Trucksess, M.W.; Stack, M.E.; Nesheim, S.; Page, S.W. and Albert, R.H. (1991), Immunoaffinity column coupled with solution fluorometry or liquid chromatography postcolumn derivatization for determination of aflatoxins in corn, peanuts, and peanut butter: Collaborative study. Journal of the Association of Official Analytical Chemists, 74, 81-88.
21. WHO (World Health Organization). Mycotoxins. Geneva: UNEP/WHO, 1979, p.127.