Alternative method for direct DNA probe labeling and detection using the checkerboard hybridization format

Journal of Clinical Microbiology

Volumn 48, Issue 8, 2010, Pages 3039-3040

  Do Nascimento, Cássio ^a   De Albuquerque Jr , Rubens Ferreira ^a   Monesi, Nadia ^a   Candido Silva, Juliana Aparecida ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

GENOMIC DNA; BACTERIAL DNA; OLIGONUCLEOTIDE PROBE;

AGGREGATIBACTER ACTINOMYCETEMCOMITANS; ASPERGILLUS FUMIGATUS; CALIBRATION; CAPNOCYTOPHAGA; CAPNOCYTOPHAGA GINGIVALIS; CAPNOCYTOPHAGA SPUTIGENA; CHEMOLUMINESCENCE; CONCENTRATION (PARAMETERS); CONTROLLED STUDY; DNA EXTRACTION; DNA HYBRIDIZATION; DNA PROBE; FUSOBACTERIUM NUCLEATUM; LABELING INDEX; LETTER; NONHUMAN; PARVIMONAS MICRA; PORPHYROMONAS GINGIVALIS; PREVOTELLA INTERMEDIA; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; STREPTOCOCCUS MUTANS; TANNERELLA FORSYTHENSIS; VEILLONELLA PARVULA; BACTERIA; BIODIVERSITY; CHEMISTRY; CLASSIFICATION; EVALUATION STUDY; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MICROBIOLOGICAL EXAMINATION; MICROBIOLOGY; MOLECULAR DIAGNOSIS; MOUTH; NUCLEIC ACID HYBRIDIZATION; OLIGONUCLEOTIDE PROBE; PROCEDURES; STAINING;

BACTERIA; BACTERIOLOGICAL TECHNIQUES; BIODIVERSITY; DNA, BACTERIAL; HUMANS; MOLECULAR DIAGNOSTIC TECHNIQUES; MOUTH; NUCLEIC ACID HYBRIDIZATION; OLIGONUCLEOTIDE PROBES; STAINING AND LABELING;

EID: 77955462271     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00390-10     Document Type: Letter

References (8)

1. Alves, F. R., J. F. Siqueira, Jr., F. L. Carmo, A. L. Santos, R. S. Peixoto, I. N. Rocas, and A. S. Rosado. 2009. Bacterial community profiling of cryogenically ground samples from the apical and coronal root segments of teeth with apical periodontitis. J. Endod. 35:486-492.
2. Baumgartner, S., T. Imfeld, O. Schicht, C. Rath, R. E. Persson, and G. R. Persson. 2009. The impact of the stone age diet on gingival conditions in the absence of oral hygiene. J. Periodontol. 80:759-768.
3. De Souza, R. F., C. Nascimento, R. R. Regis, C. H. Silva-Lovato, and H. F. Paranhos. 2009. Effects of the domestic use of a disclosing solution on the denture biofilm: a preliminary study. J. Oral Rehabil. 36:491-497.
4. do Nascimento, C., R. F. Albuquerque, Jr., J. P. M. Issa, I. Y. Ito, C. H. Lovato da Silva, H. F. Paranhos, and R. F. de Souza. 2009. Use of the DNA checker-board hybridization method for detection and quantitation of Candida species in oral microbiota. Can. J. Microbiol. 55:622-626.
5. Papaioannou, W., S. Gizani, A. D. Haffajee, M. Quirynen, E. Mamai-Homata, and L. Papagiannoulis. 2009. The microbiota on different oral surfaces in healthy children. Oral Microbiol. Immunol. 24:183-189.
6. Pitcher, D. G., N. A. Sanders, and R. J. Owen. 1989. Rapid extraction of bacterial genomic DNA with guanidium thiocyanate. Lett. Appl. Microbiol. 8:151-156.
7. Sakamoto, M., J. F. Siqueira, Jr., I. N. Rocas, and Y. Benno. 2009. Diversity of spirochetes in endodontic infections. J. Clin. Microbiol. 47:1352-1357.
8. Socransky, S. S., C. Smith, L. Martin, B. J. Paster, F. E. Dewhirst, and A. E. Levin. 1994. "Checkerboard" DNA-DNA hybridization. Biotechniques 17:788-792.