mRNA levels of CD31, CD144, CD146 and von Willebrand factor do not serve as surrogate markers for circulating endothelial cells

Thrombosis and Haemostasis

Volumn 104, Issue 2, 2010, Pages 318-326

  Strijbos, Michiel H ^a   Van Krimpen, Brigitte A ^a   Debets, Reno ^a   Kraan, Jaco ^a   Sleijfer, Stefan ^a   Gratama, Jan Willem ^a   Lamers, Cor H J ^a  

^a ERASMUS MEDICAL CENTER   (Netherlands)

Author keywords

Circulating endothelial cells; Endothelial markers; RT PCR; Vascular damage

Indexed keywords

BIOLOGICAL MARKER; CD146 ANTIGEN; CD31 ANTIGEN; MESSENGER RNA; VASCULAR ENDOTHELIAL CADHERIN; VON WILLEBRAND FACTOR;

ADULT; ARTICLE; BLOOD SAMPLING; CELL FRACTIONATION; CELL SUSPENSION; CONTROLLED STUDY; ENDOTHELIUM CELL; FEASIBILITY STUDY; FEMALE; FLOW CYTOMETRY; HUMAN; HUMAN CELL; MALE; PRIORITY JOURNAL; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION;

ADULT; ANTIGENS, CD; ANTIGENS, CD146; ANTIGENS, CD31; APOPTOSIS; BIOLOGICAL MARKERS; CADHERINS; CELLS, CULTURED; ENDOTHELIAL CELLS; FEASIBILITY STUDIES; FEMALE; FLOW CYTOMETRY; HUMANS; IMMUNOMAGNETIC SEPARATION; MALE; MIDDLE AGED; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; T-LYMPHOCYTES; VON WILLEBRAND FACTOR;

EID: 77955448903     PISSN: 03406245     EISSN: None     Source Type: Journal    
DOI: 10.1160/TH09-08-0594     Document Type: Article

References (29)

1. Bicknell R, Harris AL. Mechanisms and therapeutic implications of angiogenesis. Curr Opin Oncol 1996; 8: 60-65.
2. Folkman J. Angiogenesis - retrospect and outlook. Exs 1992; 61: 4-13.
3. Kabbinavar F, Hurwitz HI, Fehrenbacher L, et al. Phase II, randomized trial comparing bevacizumab plus fluorouracil (FU)/leucovorin (LV) with FU/LV alone in patients with metastatic colorectal cancer. J Clin Oncol 2003; 21: 60-65.
4. Motzer RJ, Hutson TE, Tomczak P, et al. Sunitinib versus interferon alfa in metastatic renal-cell carcinoma. New Engl J Med 2007; 356: 115-124. (Pubitemid 46089672)
5. Sandler A, Gray R, Perry MC, et al. Paclitaxel-carboplatin alone or with bevacizumab for non-small-cell lung cancer. New Engl J Med 2006; 355: 2542-2550.
6. Patterson DM, Rustin GJ. Vascular damaging agents. Clinical Oncol 2007; 19: 443-456.
7. Van De Wiele C, Oltenfreiter R, De Winter O, et al. Tumour angiogenesis pathways: related clinical issues and implications for nuclear medicine imaging. Eur J Nucl Med Mol Imag 2002; 29: 699-709.
8. Strijbos MH, Verhoef C, Gratama JW, et al. On the origin of (CD105+) circulating endothelial cells. Thromb Haemost 2009; 102: 347-351.
9. Khan SS, Solomon MA, McCoy JP, Jr. Detection of circulating endothelial cells and endothelial progenitor cells by flow cytometry. Cytometry 2005; 64: 1-8.
10. Blann AD, Woywodt A, Bertolini F, et al. Circulating endothelial cells. Biomarker of vascular disease. Thromb Haemost 2005; 93: 228-235.
11. Goon PK, Boos CJ, Stonelake PS, et al. Detection and quantification of mature circulating endothelial cells using flow cytometry and immunomagnetic beads: a methodological comparison. Thromb Haemost 2006; 96: 45-52.
12. Redondo S, Hristov M, Gumbel D, et al. Biphasic effect of pioglitazone on isolated human endothelial progenitor cells: involvement of peroxisome proliferator-activated receptor-gamma and transforming growth factor-beta1. Thromb Haemost 2007; 97: 979-987.
13. Beerepoot LV, Mehra N, Vermaat JS, et al. Increased levels of viable circulating endothelial cells are an indicator of progressive disease in cancer patients. Ann Oncol 2004; 15: 139-145.
14. Rowand JL, Martin G, Doyle GV, et al. Endothelial cells in peripheral blood of healthy subjects and patients with metastatic carcinomas. Cytometry A 2007; 71: 105-113.
15. Strijbos MH, Gratama JW, Kraan J, et al. Circulating endothelial cells in oncology: pitfalls and promises. Br J Cancer 2008; 98: 1731-1735.
16. Strijbos MH, Rao C, Schmitz PI, et al. Correlation between circulating endothelial cell counts and plasma thrombomodulin levels as markers for endothelial damage. Thromb Haemost 2008; 100: 642-647.
17. Elshal MF, Khan SS, Takahashi Y, et al. CD146 (Mel-CAM), an adhesion marker of endothelial cells, is a novel marker of lymphocyte subset activation in normal peripheral blood. Blood 2005; 106: 2923-2924.
18. Kraan J, Gratama JW, Keeney M, et al. Setting up and calibration of a flow cytometer for multicolor immunophenotyping. J Biol Regul Homeost Agents 2003; 17: 223-233.
19. Chan EC, Fraser S, Yin S, et al. Human myometrial genes are differentially expressed in labor: a suppression subtractive hybridization study. J Clin Endocrinol Metabol 2002; 87: 2435-2441.
20. Gabert J, Beillard E, van der Velden VH, et al. Standardization and quality control studies of 'real-time' quantitative reverse transcriptase polymerase chain reaction of fusion gene transcripts for residual disease detection in leukemia - a Europe Against Cancer program. Leukemia 2003; 17: 2318-2357.
21. Strijbos MH, Kraan J, den Bakker MA, et al. Cells meeting our immunophenotypic criteria of endothelial cells are large platelets. Cytometry 2007; 72: 86-93.
22. Dignat-George F, Sabatier F, Blann A, et al. Detection of circulating endothelial cells: CD146-based magnetic separation enrichment or flow cytometric assay? J Clin Oncol 2007; 25: e1-2; author's reply e3-5.
23. Smirnov DA, Foulk BW, Doyle GV, et al. Global gene expression profiling of circulating endothelial cells in patients with metastatic carcinomas. Cancer Res 2006; 66: 2918-2922.
24. Furstenberger G, von Moos R, Senn HJ, et al. Real-time PCR of CD146 mRNA in peripheral blood enables the relative quantification of circulating endothelial cells and is an indicator of angiogenesis. Br J Cancer 2005; 93: 793-798.
25. Foreman KE, Tang J. Molecular mechanisms of replicative senescence in endothelial cells. Experimental Gerontol 2003; 38: 1251-1257.
26. Woywodt A, Streiber F, de Groot K, et al. Circulating endothelial cells as markers for ANCA-associated small-vessel vasculitis. Lancet 2003; 361: 206-210. (Pubitemid 36126220)
27. Solovey A, Gui L, Ramakrishnan S, et al. Sickle cell anemia as a possible state of enhanced anti-apoptotic tone: survival effect of vascular endothelial growth factor on circulating and unanchored endothelial cells. Blood 1999; 93: 3824-3830.
28. Aoudjit F, Vuori K. Matrix attachment regulates Fas-induced apoptosis in endothelial cells: a role for c-flip and implications for anoikis. J Cell Biol 2001; 152: 633-643.
29. Rabascio C, Muratori E, Mancuso P, et al. Assessing tumor angiogenesis: increased circulating VE-cadherin RNA in patients with cancer indicates viability of circulating endothelial cells. Cancer Res 2004; 64: 4373-4377.