Highly sensitive determination of recombinant human erythropoietin-α in aptamer-based affinity probe capillary electrophoresis with laser-induced fluorescence detection

Journal of Chromatography A

Volumn 1217, Issue 35, 2010, Pages 5635-5641

  Shen, Rui ^a   Guo, Lei ^a   Zhang, Zhaoyang ^a   Meng, Qingwei ^a   Xie, Jianwei ^a  

^a BEIJING INSTITUTE OF PHARMACOLOGY AND TOXICOLOGY   (China)

Author keywords

Affinity probe capillary electrophoresis; Aptamer; Laser induced fluorescence; RHuEPO

Indexed keywords

AFFINITY PROBE CAPILLARY ELECTROPHORESIS; APTAMERS; FLUORESCEIN ISOTHIOCYANATE; FLUORESCENCE INTENSITIES; HIGH VOLTAGE; HIGHLY SENSITIVE; HUMAN ERYTHROPOIETIN; HUMAN SERUM; LASER INDUCED FLUORESCENCE; LASER INDUCED FLUORESCENCE DETECTION; LIMIT OF DETECTION; LINEAR RANGE; OPTIMIZED CONDITIONS; PEAK HEIGHT; PHYSIOLOGICAL BUFFERS; RECOMBINANT GLYCOPROTEINS; SAMPLE BUFFER; SEPARATION EFFICIENCY; SINGLE-STRANDED DNA; SODIUM CATIONS; SPECIFIC INTERACTION; SYSTEMATIC OPTIMIZATION;

BODY FLUIDS; CAPILLARY ELECTROPHORESIS; ELECTROCHEMISTRY; FLUORESCENCE; LASER PRODUCED PLASMAS; OPTIMIZATION;

PROBES;

6 CARBOXYFLUORESCEIN; APTAMER; FLUORESCEIN ISOTHIOCYANATE; RECOMBINANT ERYTHROPOIETIN; SINGLE STRANDED DNA; SODIUM ION; DNA PROBE; ERYTHROPOIETIN; FLUORESCENT DYE; RECOMBINANT PROTEIN;

AFFINITY PROBE CAPILLARY ELECTROPHORESIS; ARTICLE; BLOOD ANALYSIS; CAPILLARY ELECTROPHORESIS; COMPLEX FORMATION; CONTROLLED STUDY; DEGLYCOSYLATION; DISSOCIATION CONSTANT; FLUORESCENCE ANALYSIS; HUMAN; LASER INDUCED FLUORESCENCE; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROCESS OPTIMIZATION; QUANTITATIVE ANALYSIS; SENSITIVITY AND SPECIFICITY; URINALYSIS; BLOOD; CHEMISTRY; DEVICES; DNA PROBE; EVALUATION STUDY; FLUORESCENCE; ISOLATION AND PURIFICATION; LASER; LIMIT OF DETECTION; PROCEDURES; URINE;

APTAMERS, NUCLEOTIDE; DNA PROBES; ELECTROPHORESIS, CAPILLARY; ERYTHROPOIETIN, RECOMBINANT; FLUORESCENCE; FLUORESCENT DYES; HUMANS; LASERS; LIMIT OF DETECTION; ERYTHROPOIETIN; RECOMBINANT PROTEINS;

EID: 77955425309     PISSN: 00219673     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.chroma.2010.06.072     Document Type: Article

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