Characterization of two subpopulations of the PICM-19 porcine liver stem cell line for use in cell-based extracorporeal liver assistance devices

International Journal of Artificial Organs

Volumn 33, Issue 6, 2010, Pages 348-361

  Talbot, Neil C ^a   Caperna, Thomas J ^a   Willard, Ryan R ^a   Meekin, John H ^b   Garrett, Wesley M ^a  

^a AGRICULTURAL RESEARCH SERVICE   (United States)

^b HepaLife Technologies Inc ^*  (United States)

Author keywords

Bioengineering; Cell line; Culture; Hepatocyte; Liver

Indexed keywords

AMINO ACIDS; AMMONIA; ARTIFICIAL ORGANS; BIOLOGICAL ORGANS; BODY FLUIDS; CELL MEMBRANES; DETOXIFICATION; METABOLISM; MONOLAYERS; PROTEINS; STEM CELLS; UREA;

ARTIFICIAL LIVER; BIOENGINEERING; CELL DENSITY; CELL LINES; CULTURE; DOUBLING TIME; HEPATOCYTES; LIVER DEVICES; SERUM PROTEINS; STEM-CELL;

CELL CULTURE;

AMMONIA; CYTOCHROME P450; GAMMA GLUTAMYLTRANSFERASE; GLUTAMINE; UREA;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL DENSITY; CELL DIFFERENTIATION; CELL GROWTH; CELL LINE; CELL STRUCTURE; CELL SUBPOPULATION; CULTURE MEDIUM; DETOXIFICATION; ENZYME ACTIVITY; GOLGI COMPLEX; LIVER CELL; LIVER STEM CELL LINE PICM 19B; LIVER STEM CELL LINE PICM 19H; LIVER SUPPORT; MITOCHONDRION; NONHUMAN; PROTEIN EXPRESSION; ROUGH ENDOPLASMIC RETICULUM; SMOOTH ENDOPLASMIC RETICULUM; STEM CELL; SWINE; TRANSMISSION ELECTRON MICROSCOPY;

EID: 77955363533     PISSN: 03913988     EISSN: None     Source Type: Journal    
DOI: 10.1177/039139881003300603     Document Type: Article

References (44)

1. Brooks FA, Gardner RL. The origin and efficient derivation of embryonic stem cells in the mouse. Proc Natl Acad Sci USA 1997; 94: 5709-12.
2. Talbot NC, Rexroad CE, Pursel VG, Powell AM, Nel ND. Culturing the epiblast cells of the pig blastocyst. In Vitro Cell Dev Biol 1993; 29A: 543-54.
3. Talbot NC, Rexroad CE, Powell A, et al. A continuous culture of pluripotent fetal hepatocytes derived from the 8-day epiblast of the pig. In Vitro Cell Dev Biol 1994a; 30A: 843-50.
4. Talbot NC, Pursel VG, Rexroad CE, Caperna TJ, Powell AM, Stone RT. Colony isolation and secondary culture of fetal porcine hepatocytes on STO feeder cells. In Vitro Cell Dev Biol 1994b; 30A: 851-8.
5. Talbot NC, Caperna TJ, Lebow LT, Moscioni D, Pursel VG, Rexroad, CE. Ultrastructure, enzymatic, and transport properties of the PICM-19 bipotent liver cell line. Exp Cell Res 1996a; 225: 22-34. (Pubitemid 26158452)
6. Talbot NC, Caperna TJ. Selective and organotypic culture of intrahepatic bile duct cells from adult pig liver. In Vitro Cell Dev Biol 1998; 34A: 785-98.
7. Talbot NC, Caperna TJ, Wells KD. The PICM-19 cell line as an in vitro model of liver bile ductules: effects of cAMP inducers, biopeptides and pH. Cells Tissues Organs 2002; 171: 99-116.
8. Ishii M, Vroman B, LaRusso NF. Isolation and morphologic characterization of bile duct epithelial cells from normal rat liver. Gastroenterology 1989; 97: 1236-47. (Pubitemid 19258709)
9. Tanaka M. A histochemical study on the activity of gamma-glytamyl transpeptidase in liver disease. Acta Path Jpn 1974; 24: 651-65.
10. Sussman NL, Kelly JH. The artificial liver. Sci Am 1995; 2: 68-77.
11. Strain AJ, Neuberger JM. A bioartificial liver - state of the art. Science 2002; 295: 1005-9.
12. Sen S, Williams R. New liver support devices in acute liver failure: a critical evaluation. Semin Liver Dis 2003; 23: 283-94. (Pubitemid 37052453)
13. Talbot NC, Paape MJ. Continuous culture of pig tissue-derived macrophages. Methods Cell Sci 1996b; 18: 315-27.
14. Donato MT, Gómez-Lechón MJ, Castell JV. A microassay for measuring cytochrome P450IA1 and P450IIB1 activities in intact human and rat hepatocytes cultured on 96-well plates. Anal Biochem 1993; 213: 29-33.
15. Talbot NC, Blomberg le A, Mahmood A, Caperna TJ, Garrett WM. Isolation and characterization of porcine visceral endoderm cell lines derived from in vivo 11-day blastocysts. In Vitro Cell Dev Biol Anim 2007; 43: 72-86.
16. Talbot NC, Powell A, Garrett W, Edwards JL, Rexroad CE. Ultrastructural and karyotypic examination of in vitro produced bovine embryos developed in the sheep uterus. Tissue Cell 2000a; 32: 9-27.
17. Alberts B, Bray D, Lewis J, Raff M, Roberts K, Watson, JD. Molecular Biology of the Cell. 2nd ed. New York: Garland Publishing; 1994: 789, 954-5.
18. Gilloteaux J, Karkare S, Kelly T, Hawkins WS. Ultrastructural aspects of human gallbladder epithelial cells in cholelithiasis: production of anionic mucus. Microsc Res Tech 1997; 38: 643-59.
19. Talbot NC, Caperna TJ, Edwards JL, Garrett W, Wells KD, Ealy AD. Establishment of bovine blastocyst-derived trophectoderm and endoderm cell lines: use of interferon-tau and serum protein expression as respective in vitro markers. Biol Reprod 2000b; 62: 235-47.
20. Wanson J C, Drochmans P, Mosselmans R, Ronveaux MF. Adult rat hepatocytes in primary monolayer culture: Ultrastructural characteristics of intercellular contacts and cell membrane differentiations. J Cell Biol 1977; 74: 858-77.
21. Singh A, Shahidi E. Ultrastructure of piglet liver. In: Tumbleson ME, ed. Swine in Biomedical Research. Vol. 1. New york: Plenum Press; 1987: 84.
22. Enzan H, Ohkita T, Fujuta H, Iijima S. Light and electron microscopic studies on the development of periportal bile ducts of the human embryo. Acta Pathol Jpn 1974; 24: 427-47.
23. Nakanuma Y, Katayanagi K, Kawamura Y, Yoshida K. Monolayer and three-dimensional cell culture and living tissue culture of gallbladder epithelium. Microsc Res Tech 1997; 39: 71-84.
24. Meister A, Tate SS, Leonard RL. Membrane-bound gamma- glutamyltranspeptidase. In: Martinosi A, ed. The Enzymes of Biological Membranes. New York: Plenum; 1976: 315-47.
25. Murray GI, Barnes TS, Sewell HF, et al. Cytochrome P-450 localization in normal human adult and foetal liver by immunocytochemistry using a monoclonal antibody against human cytochrome P-450. Histochem J 1987; 19: 537-45.
26. Brill S, Holst P, Sigal S, et al. Hepatic progenitor populations in embryonic, neonatal, and adult liver. Proc Soc Exp Bio Med 1993; 204: 261-9.
27. Sirica AE. Biology of biliary epithelial cells. Prog Liver Dis 1992; 10: 63-87.
28. Alpini G, Phillips JO, LaRusso NF. The biology of biliary epithelia. In: Arias IM, Boyer JL, Fasto N, Jakoby WB, Schachter DA, Shafritz DA, eds. The Liver: Biology and Pathobiology, 3rd ed. New York: Raven Press; 1994: 623-53.
29. Willard RR, Shappell NW, Meekin JH, Talbot NC, Caperna TJ. Cytochrome P450 expression profile of the PICM-19H pig liver cell line: potential application to rapid liver toxicity assays. In Vitro Cell Dev Biol Anim 2009 Nov 14. [Epub ahead of print]
30. Triebwasser KC, Freedland RA. The effect of glucagon on ureagenesis from ammonia by isolated rat hepatocytes. Biochem Biophys Res Commun 1977; 76: 1159-65.
31. Jungermann K, Katz N. Functional specialization of different hepatocyte populations. Physiol Rev 1989; 69: 708-64.
32. Van Eyken P, Desmet VJ. Bile duct cells. In: LeBouton AV, ed. Molecular and Cell Biology of the Liver. Baton Raton, Florida: CRC Press; 1993: 475-524.
33. Newsome PN, Hussain MA, Theise ND. Hepatic oval cells: helping redefine a paradigm in stem cell biology. Curr Top Dev Biol 2004; 61: 1-28.
34. Sigal SH, Brill S, Fiorino AS, Reid LM. The liver as a stem cell and lineage system. Am J Physiol 1992; 263: G139-48.
35. Junqueira CL, Carneiro J, Kelly RO. Basic Histology. Norwalk, CT: Appleton and Lange; 1992: 406.
36. Chamuleau RA, Deurholt T, Hoekstra R. Which are the right cells to be used in a bioartificial liver? Metab Brain Dis 2005; 20: 327-35.
37. Nyberg SL, Remmel RP, Mann HJ, Peshwa MV, Hu WS, Cerra FB. Primary hepatocytes outperform Hep G2 cells as the source of biotransformation functions in a bioartificial liver. Ann Surg 1994; 220: 59-67.
38. Wang L, Sun J, Li L, Mears D, Horvat M, Sheil AG. Comparison of porcine hepatocytes with human hepatoma (C3A) cells for use in a bioartificial liver support system. Cell Transplant 1998; 7: 459-68.
39. Kobayashi N, Okitsu T, Nakaji S, Tanaka N. Hybrid bioartificial liver: establishing a reversibly immortalized human hepatocyte line and developing a bioartificial liver for practical use. J Artif Organs 2003a; 6: 236-44.
40. Kobayashi N, Okitsu T, Tanaka N. Cell choice for bioartificial livers. Keio J Med 2003b; 52: 151-7.
41. Rodríguez-Antona C, Donato MT, et al. Cytochrome P450 expression in human hepatocytes and hepatoma cell lines: molecular mechanisms that determine lower expression in cultured cells. Xenobiotica 2002; 32: 505-20.
42. Filippiet C, Keatch SA, Rangar D, Nelson LJ, Hayes PC, Plevris JN. Improvement of C3A cell metabolism for usage in bioartificial liver support systems. J Hepatol 2004; 41: 599-605.
43. Hoekstra R, Chamuleau RA. Recent developments on human cell lines for the bioartificial liver. Int J Artif Organs 2002; 25: 182-91.
44. Demetriou AA, Brown RS, Busuttil RW, et al. Prospective, randomized, multicenter, controlled trial of bioartificial liver in treating acute liver failure. Ann Surg 2004; 239: 660-70.