Combination of normal-phase medium-pressure liquid chromatography and high-performance counter-current chromatography for preparation of ginsenoside-Ro from panax ginseng with high recovery and efficiency

Separation and Purification Technology

Volumn 73, Issue 3, 2010, Pages 397-402

  Cheng, Yijun ^a,b   Liang, Qionglin ^b   Hu, Ping ^a   Wang, Yiming ^b   Jun, Frank Wu ^c   Luo, Guoan ^a,b  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b TSINGHUA UNIVERSITY   (China)

^c Meditrina   (United Kingdom)

Author keywords

Ginsenoside Ro; High performance counter current chromatography; Normal phase medium pressure liquid chromatography; Oleanane type saponins; Panax ginseng

Indexed keywords

GINSENOSIDES; HIGH-PERFORMANCE COUNTER-CURRENT CHROMATOGRAPHY; MEDIUM-PRESSURE LIQUID CHROMATOGRAPHY; OLEANANE; PANAX GINSENG; COUNTER CURRENT; GINSENOSIDE-RO; IMMUNOMODULATORY ACTIVITIES; IONIZATION MASS SPECTROMETRY; OLEANANE-TYPE SAPONINS;

CHROMATOGRAPHY; ELECTROSPRAY IONIZATION; ESTERS; LIQUIDS; MASS SPECTROMETRY; METABOLITES; ORGANIC ACIDS; RECOVERY;

HIGH PRESSURE LIQUID CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY;

PANAX GINSENG;

EID: 77955329364     PISSN: 13835866     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.seppur.2010.04.029     Document Type: Article

References (30)

1. A.S. Attelt, J.A. Wu, and C.-S. Yuan Ginseng pharmacology: multiple constituents and multiple actions Biochem. Pharmacol. 58 1999 1685 1693
2. P. Hu, G.A. Luo, Q. Wang, Z.-Z. Zhao, W. Wang, and Z.-H. Jiang The retention behavior of ginsenosides in HPLC and its application to quality assessment of radix ginseng Arch. Pharmacol. Res. 31 2008 1265 1273
3. N. Fuzzati, B. Gabetta, K. Jayakar, R. Pace, and F. Peterlongo Liquid chromatography-electrospray mass spectrometric identification of ginsenosides in Panax ginseng roots J. Chromatogr. A 854 1999 69 79
4. H. Matsuda, K. Samkawa, and M. Kubo Anti-inflammatory activity of ginsenoside Ro Planta Med. 56 1990 19 23
5. C.-M. Teng, S.-C. Kuo, F.-N. Ko, J.-C. Lee, L.-G. Lee, S.-C. Chen, and T.-F. Huang Antiplatelet actions of panaxynol and ginsenosides isolated from ginseng Biochim. Biophys. Acta 990 1989 315 320
6. D.S. Kim, S.R. Oh, I.S. Lee, K.Y. Jung, J.D. Park, S.I.I. Kim, and H.K. Lee Anticomplementary activity of ginseng saponins and their degradation products Phytochemistry 47 1998 397 399
7. J.-L. Yu, D.-Q. Dou, X.-H. Chen, H.-Z. Yang, X.-Y. Hu, and G.-F. Cheng Ginsenoside-Ro enhances cell proliferation and modulates Th1/Th2 cytokines production in murine splenocytes Acta Pharm. Sinica 40 2005 332 336
8. G.A. Luo, Q.L. Liang, and R.L. Zhang Study of chemical matteromics and prescription of traditional Chinese medicine - and an analysis of material foundation of compound prescription Qingkailing Sci. Technol. Mod. Tradit. Chin. Med. 8 2006 6 15
9. Z.G. Hou, J.G. Luo, and L.Y. Kong Medium-pressure liquid chromatography coupled to electrospray ionization mass spectrometry for separation and on-line characterization of flavonoids from Asparagus officinalis Chromatographia 70 2009 1447 1450
10. O. Sticher Nature product isolation Nat. Prod. Rep. 25 2008 517 554
11. H. Kanazawa, Y. Nagata, Y. Matsushima, M. Tomoda, and N. Takai Preparative high-performance liquid chromatography on chemically modified porous glass. Isolation of saponins from ginseng Chem. Pharm. Bull. 38 1990 1630 1632
12. Y.Y. Jiang, W.Q. Zhao, C. Feng, T.T. Zhou, G.R. Fan, and Y.T. Wu Isolation and purification of isoflavonoids from Rhizoma Belamcandae by two-dimensional preparative high-performance liquid chromatography with column switch technology Biomed. Chromatogr. 23 2009 1064 1072
13. M. Kracun, A. Kocijan, A. Bastarda, R. Grahek, J. Plavec, and D. Kocjan Isolation and structure determination of oxidative degradation products of atorvastatin J. Pharm. Biomed. Anal. 50 2009 729 736
14. Y. Ito, and R.L. Bowman Countercurrent chromatography: liquid-liquid partition chromatography without solid support Science 167 1970 281 283
15. A. Berthod, M.J. Ruiz-Ángel, and S. Carda-Broch Countercurrent chromatography: people and applications J. Chromatogr. A 1216 2009 4206 4217
16. I.A. Sutherland Recent progress on the industrial scale-up of counter-current chromatography J. Chromatogr. A 1151 2007 6 13
17. I.A. Sutherland, P. Hewitson, and S. Ignatova New 18-l process-scale counter-current chromatography centrifuge J. Chromatogr. A 1216 2009 4201
18. I.A. Sutherland, and D. Fisher Role of counter-current chromatography in the modernisation of Chinese herbal medicines J. Chromatogr. A 1216 2009 740 753
19. Y.W. Ha, S.S. Lim, I.J. Ha, Y.C. Na, J.J. Seo, H. Shin, S.H. Son, and Y.S. Kim Preparative isolation of four ginsenosides from Korean red ginseng (steam-treated Panax ginseng C. A. Meyer), by high-speed counter-current chromatography coupled with evaporative light scattering detection J. Chromatogr. A 1151 2007 37 44
20. X.-L. Cao, Y. Tian, T.-Y. Zhang, Q.-H. Liu, L.-J. Jia, and Y. Ito Separation of Dammarane-Saponins from Notoginseng, Root of Panax notoginseng (Burk.) F. H. Chen, by HSCCC coupled with evaporative light scattering detector J. Liq. Chromatogr. Rel. Technol. 26 2003 1579 1591
21. Q. Du, G. Jerz, R. Waibel, and P. Winterhalter Isolation of dammarane saponins from Panax notoginseng by high-speed counter-current chromatography J. Chromatogr. A 1008 2003 173 180
22. L. Zhu, H. Li, Y. Liang, X. Wang, H. Xie, T. Zhang, and Y. Ito Application of high-speed counter-current chromatography and preparative high-performance liquid chromatography mode for rapid isolation of anthraquinones from Morinda officinalis How. Sep. Purif. Technol. 70 2009 147 152
23. S. Yao, J. Luo, X. Huang, and L. Kong Application of preparative high-speed counter-current chromatography/preparative high-performance liquid chromatography mode in rapid separation of saponins J. Chromatogr. B 864 2008 69 77
24. H.Y. Ye, S. Ignatova, H. Luo, Y.F. Li, A.H. Peng, L.J. Chen, and I.A. Sutherland Preparative separation of a terpenoid and alkaloids from Tripterygium wilfordii Hook. f. using high-performance counter-current chromatography: comparison of various elution and operating strategies J. Chromatogr. A 1213 2008 145 153
25. P. Hewitson, S. Ignatova, H.Y.L.J. Chen, and I.A. Sutherland Intermittent counter-current extraction as an alternative approach to purification of Chinese herbal medicine J. Chromatogr. A 1216 2009 4187 4192
26. M. Zhang, S. Ignatova, Q.L. Liang, F. Wujun, I.A. Sutherland, Y.M. Wang, and G.A. Luo Rapid and high-throughput purification of salvianolic acid B from Salvia miltiorrhiza Bunge by high-performance counter-current chromatography J. Chromatogr. A 1216 2009 3869 3873
27. Y. Ito Golden rules and pitfalls in selecting optimum conditions for high-speed counter-current chromatography J. Chromatogr. A 1065 2005 145 168
28. Q.-Z. Du, C.-J. Wu, J.-G. Qian, P.-D. Wu, and Y. Ito Relationship between the flow-rate of the mobile phase and retention of the stationary phase in counter-current chromatography J. Chromatogr. A 835 1999 231 235
29. I.A. Sutherland, A.J. Booth, L. Brown, B. Kemp, H. Kidwell, D. Games, A.S. Graham, G.G. Guillon, D. Hawes, M. Hayes, L. Jananway, G.J. Lye, P. Massey, C. Preston, P. Shering, T. Shoulder, C. Strawson, and P. Wood Industrial scale-up of countercurrent chromatography J. Liq. Chromatogr. Rel. Technol. 24 2001 1533 1553
30. C.-H. He, and C.-X. Zhao Retention of the stationary phase for high-speed countercurrent chromatography AIChE J. 53 2007 1460 1471