Comparative study of Gram stain, potassium hydroxide smear, culture and nested PCR in the diagnosis of fungal keratitis

Ophthalmic Research

Volumn 44, Issue 4, 2010, Pages 251-256

  Badiee, Parisa ^a   Nejabat, Mahmood ^b   Alborzi, Abdolvahab ^a   Keshavarz, Fatemeh ^b   Shakiba, Elaheh ^a  

^a SHIRAZ UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^b SHIRAZ UNIVERSITY OF MEDICAL SCIENCES   (Iran)

Author keywords

Aspergillus; Aspergillus flavus; Candida albicans; Fusarium solani; Nested PCR

Indexed keywords

ANTIBIOTIC AGENT; ANTIFUNGAL AGENT; CORTICOSTEROID; POTASSIUM HYDROXIDE;

ADOLESCENT; ADULT; AGED; ARTICLE; ASPERGILLUS FUMIGATUS; CANDIDA ALBICANS; CHILD; CLINICAL ARTICLE; COMPARATIVE STUDY; CONTROLLED STUDY; CORNEA; CORTICOSTEROID THERAPY; DRUG EFFICACY; ENDOPHTHALMITIS; EVISCERATION; FEMALE; FUNGUS CULTURE; FUSARIUM SOLANI; GRAM STAINING; HUMAN; HUMAN TISSUE; HYPOPYON; KERATOMYCOSIS; MALE; MICROBIOLOGICAL EXAMINATION; PENETRATING KERATOPLASTY; POLYMERASE CHAIN REACTION; PRESCHOOL CHILD; PRIORITY JOURNAL; SCHOOL CHILD; SMEAR; STANDARD;

ADULT; AGED; AGED, 80 AND OVER; ASPERGILLUS FUMIGATUS; BACTERIOLOGICAL TECHNIQUES; CANDIDA ALBICANS; CHILD; CORNEAL ULCER; DNA, FUNGAL; EYE INFECTIONS, FUNGAL; FEMALE; FUNGI; FUSARIUM; GENTIAN VIOLET; HUMANS; HYDROXIDES; MALE; MIDDLE AGED; PHENAZINES; POLYMERASE CHAIN REACTION; POTASSIUM COMPOUNDS; STAINING AND LABELING; YOUNG ADULT;

EID: 77955292402     PISSN: 00303747     EISSN: None     Source Type: Journal    
DOI: 10.1159/000313988     Document Type: Article

References (24)

1. Gopinathan U, Garg P, Fernandes M, Sharma S, Athmanathan S, Rao GN: The epidemiological features and laboratory results of fungal keratitis: a 10-year review at a referral eye care centre in south India. Cornea 2002; 21: 555-559.
2. Chowdhary A, Singh K: Spectrum of fungal keratitis in north India. Cornea 2005; 24: 8-15.
3. Klotz SA, Penn CC, Negvesky GJ, Butrus SI: Fungal and parasitic infections of the eye. Clin Microbiol Rev 2000; 13: 662-685.
4. Thomas PA: Current perspectives on ophthalmic mycoses. Clin Microbiol Rev 2003; 16: 730-797.
5. Dahlgren MA, Lingappan A, Wilhelmus KR: The clinical diagnosis of microbial keratitis. Am J Ophthalmol 2007; 143: 940-944.
6. Alfonso EC, Rosa RH, Miller D: Fungal keratitis; in Krachmer J, Mannis M, Holland E (eds): Fundamental, Diagnosis and Management of Cornea. Philadelphia, Elsevier Mosby, 2005, p 1105.
7. Gaudio PA, Gopinathan U, Sangwan V, Hughes TE: Polymerase chain reaction based detection of fungi in infected cornea. Br J Ophthalmol 2002; 86: 755-760.
8. Jaeger EE, Carroll NM, Choudhury S, Dunlop AA, Towler HM, Matheson MM, Adamson P, Okhravi N, Lightman S: Rapid detection and identification of Candida , Aspergillus , and Fusarium species in ocular samples using nested PCR. J Clin Microbiol 2000; 38: 2902-2908.
9. Anand A, Madhavan H, Neelam V, Lily T: Use of polymerase chain reaction in the diagnosis of fungal endophthalmitis. Ophthalmology 2001; 108: 326-330. (Pubitemid 32115964)
10. Vengayil S, Panda A, Satpathy G, Nayak N, Ghose S, Patanaik D, Khokhar S: Polymerase chain reaction-guided diagnosis of mycotic keratitis: a prospective evaluation of its efficacy and limitations. Invest Ophthalmol Vis Sci 2009; 50: 152-156.
11. Badiee P, Kordbacheh P, Alborzi A, Malekhoseini SA, Ramzi M, Mirhendi M, Mahmoodi M, Shakiba E: Study on invasive fungal infections in immunocompromised patients to present a suitable early diagnostic procedure. Int J Infect Dis 2009; 13: 97-102.
12. Yamakami Y, Hashimoto A, Tokimatsu I, Nasu M: PCR detection of DNA specific for Aspergillus species in serum of patients with invasive aspergillosis. J Clin Microbiol 1996; 34: 2464-2468.
13. Nucci M: Emerging moulds: Fusarium , Scedosporium and Zygomycetes in transplant recipients. Curr Opin Infect Dis 2003; 16: 607-612.
14. Pfaller MA, Diekema DJ: Rare and emerging opportunistic fungal pathogens: concern for resistance beyond Candida albicans and Aspergillus fumigatus. J Clin Microbiol 2004; 42: 4419-4431.
15. Thomas PA: Fungal infection of the cornea. Eye 2003; 17: 852-862.
16. Evans EGV, Richardson MD: General guidelines on laboratory diagnosis; in Evans EGV, Richardson MD (eds): Medical Mycology: A Practical Approach. Oxford, IRL Press at Oxford University Press, 1998, p 23.
17. McLeod SD, Kolahdouz-Isfahani A, Rostamian K, Flowers CW, Lee PP, McDonnell PJ: The role of smears, cultures, and antibiotic sensitivity testing in the management of suspected infectious keratitis. Ophthalmology 1996; 103: 23-28.
18. Jain AK, Bansal R, Felcida V, Rajwanshi A: Evaluation of impression smear in the diagnosis of fungal keratitis. Indian J Ophthalmol 2007; 55: 33-36.
19. Bharathi MJ, Ramakrishnan R, Vasu S, Meenakshi R, Palaniappan R: Epidemiological characteristics and laboratory diagnosis of fungal keratitis: a three-year study. Indian J Ophthalmol 2003; 51: 315-321.
20. Badiee P , Kordbacheh P, Alborzi A, Zakernia M, Haddadi P: Early detection of systemic candidiasis in the whole blood of patients with hematological malignancies. Jpn Infect Dis 2009; 62: 1-5.
21. Kumar M, Mishra NK, Shukla PK: Sensitive and rapid polymerase chain reaction based diagnosis of mycotic keratitis through single- stranded conformation polymorphism. Am J Ophthalmol 2005; 140: 851-857.
22. Kumar M, Shukla PK: Single-stranded conformation polymorphism of large subunit of ribosomal RNA is best suited to diagnosing fungal infections and differentiating fungi at species level. Diagn Microbiol Infect Dis 2006; 56: 45-51.
23. Ghosh A, Basu S, Datta H, Chattopadhyay D: Evaluation of polymerase chain reactionbased ribosomal DNA sequencing technique for the diagnosis of mycotic keratitis. Am J Ophthalmol 2007; 144: 396-403.
24. Kim E, Chidambaram JD, Srinivasan M, Lalitha P, Wee D, Lietman TM, Whitcher JP, Van Gelder RN: Prospective comparison of microbial culture and polymerase chain reaction in the diagnosis of corneal ulcer. Am J Ophthalmol 2008; 146: 714-723.