Polymerase chain reaction-guided diagnosis of mycotic keratitis: A prospective evaluation of its efficacy and limitations

Investigative Ophthalmology and Visual Science

Volumn 50, Issue 1, 2009, Pages 152-156

  Vengayil, Sujith ^a   Panda, Anita ^a   Satpathy, Gita ^a   Nayak, Niranjan ^a   Ghose, Supriyo ^a   Patanaik, Dipika ^a   Khokhar, Sudarshan ^a  

^a ALL INDIA INSTITUTE OF MEDICAL SCIENCES   (India)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIFUNGAL AGENT; POTASSIUM HYDROXIDE; RNA 28S; FUNGAL DNA; FUNGAL RNA;

ARTICLE; BACTERIUM CULTURE; CLINICAL ARTICLE; CLINICAL ASSESSMENT; CLINICAL EVALUATION; CLINICAL TRIAL; CONTROLLED STUDY; CORNEA ULCER; DIAGNOSTIC TEST; DRUG EFFICACY; EYE; FUNGUS; FUNGUS CULTURE; GRAM STAINING; HUMAN; KERATITIS; KERATOMYCOSIS; LABORATORY; MICROBIOLOGICAL EXAMINATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROSPECTIVE STUDY; RNA SEQUENCE; SENSITIVITY AND SPECIFICITY; TERTIARY HEALTH CARE; TROPIC CLIMATE; ASCOMYCETES; ASPERGILLUS FLAVUS; ASPERGILLUS FUMIGATUS; COMPARATIVE STUDY; CORNEA; FUNGAL EYE INFECTION; FUSARIUM; GENETICS; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; METHODOLOGY; MICROBIOLOGY; MYCOSIS; PREDICTION AND FORECASTING;

ASCOMYCOTA; ASPERGILLUS FLAVUS; ASPERGILLUS FUMIGATUS; BACTERIOLOGICAL TECHNIQUES; CORNEA; CORNEAL ULCER; DNA, FUNGAL; EYE INFECTIONS, FUNGAL; FALSE POSITIVE REACTIONS; FUNGI; FUSARIUM; HUMANS; MYCOSES; POLYMERASE CHAIN REACTION; PREDICTIVE VALUE OF TESTS; PROSPECTIVE STUDIES; RNA, FUNGAL; RNA, RIBOSOMAL, 28S; SENSITIVITY AND SPECIFICITY;

EID: 58249093964     PISSN: 01460404     EISSN: 15525783     Source Type: Journal    
DOI: 10.1167/iovs.07-1283     Document Type: Article

References (24)

1. Leck AK, Thomas PA, Hagan M, et al. Aetiology of suppurative corneal ulcers in Ghana and South India, and epidemiology of fungal keratitis. Br J Ophthalmol. 2002;86(11):1211-1215.
2. Upadhyay MP, Karmacharya PC, Koirala S, et al. Epidemiological characteristics, predisposing factors, and etiologic diagnosis of corneal ulceration in Nepal. Am J Ophthalmol. 1991;111(1):92-99.
3. Mino de Kaspar H, Zoulek G, Paredes ME, et al. Mycotic keratitis in Paraguay. Mycoses. 1991;34(5-6):251-254.
4. Whitcher JP, Srinivasan M, Upadhyay MP. Corneal blindness: a global perspective. Bull W H O. 2001;79(3):214 -221.
5. Gonzales CA, Srinivasan M, Whitcher JP, Smolin G. Incidence of corneal ulceration in Madurai District, South India. Ophthalmic Epidemiol. 1996;3(3):159 -166.
6. Erie JC, Nevitt MP, Hodge DO, Ballard DJ. Incidence of ulcerative keratitis in a defined population from 1950 through 1988. Arch Ophthalmol. 1993;111(12):1665-1671.
7. Van Gelder RN. Applications of the polymerase chain reaction to diagnosis of ophthalmic disease. Surv Ophthalmol. 2001;46(3): 248-258.
8. Alexandrakis G, Jalali S, Gloor P. Diagnosis of Fusarium keratitis in an animal model using the polymerase chain reaction. Br J Ophthalmol. 1998;82(3):306 -311.
9. Ferrer C, Munoz G, Alio JL, Abad JL, Colomm F. Polymerase chain reaction diagnosis in fungal keratitis caused by Alternaria alternata. Am J Ophthalmol. 2002;133(3):398 -399.
10. Anand A, Madhavan HN, Neelam V, Lily TK. Use of polymerase chain reaction in the diagnosis of fungal endophthalmitis. Ophthalmology. 2001;108(2):326 -330.
11. Lee SB, Taylor JW. Isolation of DNA from fungal mycelia and single spores In: Innis MA, Gelfand DH, Sninsky, White TJ, eds. PCR Protocols: A Guide to Methods and Applications. San Diego, CA: Academic Press; 1990:chap 34.
12. Sandhu GS, Kline BC, Stockman L, Roberts GD. Molecular probes for diagnosis of fungal infections. J Clin Microbiol. 1995;33(11): 2913-2919.
13. Thomas PA, Leck AK, Myatt M. Characteristic clinical features as an aid to the diagnosis of suppurative keratitis caused by filamentous fungi. Br J Ophthalmol. 2005;89(12):1554-1558.
14. Chowdhary A, Singh K. Spectrum of fungal keratitis in North India. Cornea. 2005;24(1):8 -15.
15. Chander J, Sharma A. Prevalence of fungal corneal ulcers in northern India. Infection. 1994;22(3):207-209.
16. Venugopal PL, Venugopal TL, Gomathi A, Ramakrishna ES, Ilavarasi S. Mycotic keratitis in Madras. Ind J Pathol Microbiol. 1989;32(3):190 -197.
17. Sharma S, Silverberg M, Mehta P, et al. Early diagnosis of mycotic keratitis: predictive value of potassium hydroxide preparation. Ind J Ophthalmol. 1998;46(1):31-35.
18. Panda A, Sharma N, Das G, Kumar N, Satpathy G. Mycotic keratitis in children: epidemiologic and microbiologic evaluation. Cornea. 1997;16(3):295-299.
19. Vajpayee RB, Angra SK, Sandramouli S, Honavar SG, Chhabra VK. Laboratory diagnosis of keratomycosis: comparative evaluation of direct microscopy and culture results. Ann Ophthalmol. 1993; 25(2):68 -71.
20. Tanure MA, Cohen EJ, Grewal S, Rapuano CJ, Laibson PR. Spectrum of fungal keratitis at Wills Eye Hospital, Philadelphia, Pennsylvania. Cornea. 2000;19(3):307-312.
21. Gaudio PA, Gopinathan U, Sangwan V, Hughes TE. Polymerase chain reaction based detection of fungi in infected corneas. Br J Ophthalmol. 2002;86(7):755-760.
22. Ghosh A, Basu S, Datta H, Chattopadhyay D. Evaluation of polymerase chain reaction-based ribosomal DNA sequencing technique for the diagnosis of mycotic keratitis. Am J Ophthalmol. 2007;144(3):396-403.
23. Bagyalakshmi R, Therese KL, Madhavan HN. Application of seminested polymerase chain reaction targeting internal transcribed spacer region for rapid detection of panfungal genome directly from ocular specimens. Ind J Ophthalmol. 2007;55(4):261-265.
24. Wiedbrauk DL, Werner JC, Drevon AM. Inhibition of PCR by aqueous and vitreous fluids. J Clin Microbiol. 1995;33(10):2643-2646.