Purification and functional characterization of human mitochondrial DNA polymerase gamma harboring disease mutations

Methods

Volumn 51, Issue 4, 2010, Pages 379-384

  Kasiviswanathan, Rajesh ^a   Longley, Matthew J ^a   Young, Matthew J ^a   Copeland, William C ^a  

^a NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES   (United States)

Author keywords

Alpers syndrome; DNA polymerase ; Mitochondrial DNA; P55 accessory subunit; Progressive external ophthalmoplegia

Indexed keywords

COMPLEMENTARY DNA; DNA DIRECTED DNA POLYMERASE GAMMA; MITOCHONDRIAL DNA; MITOCHONDRIAL ENZYME; MUTANT PROTEIN; N ETHYLMALEIMIDE; PROTEIN P55;

ANIMAL CELL; ARTICLE; BACULOVIRUS EXPRESSION SYSTEM; BINDING AFFINITY; CIRCULAR DICHROISM; CONTROLLED STUDY; DNA BINDING; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME INHIBITION; ENZYME KINETICS; ENZYME PURIFICATION; ENZYME STABILITY; GEL MOBILITY SHIFT ASSAY; GENE OVEREXPRESSION; HUMAN; IMMUNOPRECIPITATION; MITOCHONDRION; NONHUMAN; POINT MUTATION; PRIORITY JOURNAL; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; SITE DIRECTED MUTAGENESIS; STEADY STATE;

BASE SEQUENCE; DNA, MITOCHONDRIAL; DNA-DIRECTED DNA POLYMERASE; ENZYME STABILITY; HUMANS; KINETICS; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; MUTATION; PROTEIN SUBUNITS; RECOMBINANT PROTEINS;

EID: 77955278079     PISSN: 10462023     EISSN: 10959130     Source Type: Journal    
DOI: 10.1016/j.ymeth.2010.02.015     Document Type: Article

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