Assessing tetrazolium and ATP assays for rapid in situ viability quantification of bacterial cells entrapped in hydrogel beads

Enzyme and Microbial Technology

Volumn 47, Issue 4, 2010, Pages 166-173

  Wadhawan, Tanush ^a,b   McEvoy, John ^a   Prüß, Birgit M ^a   Khan, Eakalak ^a,b  

^a NORTH DAKOTA STATE UNIVERSITY   (United States)

^b North Dakota State University   (United States)

Author keywords

ATP assay; Calcium alginate; Entrapped cells; In situ viability; PPVA; XTT

Indexed keywords

ADENOSINETRIPHOSPHATE; CELLS; DISSOLUTION; ESCHERICHIA COLI; MATRIX ALGEBRA; WASTEWATER TREATMENT;

ANTIBIOTIC PRODUCTION; BACTERIAL VIABILITY; BEVERAGE PRODUCTION; CALCIUM ALGINATE; MATRIX DISSOLUTIONS; MICROBIAL VIABILITY; PPVA; QUANTITATIVE APPROACH;

CYTOLOGY;

ACID; ADENOSINE TRIPHOSPHATE; CALCIUM ALGINATE; POLYVINYL ALCOHOL; TETRAZOLIUM;

ARTICLE; BACTERIAL CELL; CELL VIABILITY; DISSOLUTION; ESCHERICHIA COLI; HEAT TREATMENT; HEATING; HIGH TEMPERATURE; HYDROGEL; MICROBIAL VIABILITY; NONHUMAN; PHOSPHORYLATION; PLATE COUNT; QUANTITATIVE ANALYSIS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 77955269229     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2010.05.003     Document Type: Article

References (43)

1. Kourkoutas Y., Bekatorou A., Banat I.M., Marchant R., Koutinas A.A. Immobilization technologies and support materials suitable in alcohol beverages production: a review. Food Microbiol 2004, 21:377-397.
2. Quintana M.G., Dalton H. Biotransformation of aromatic compounds by immobilized bacterial strains in barium alginate beads. Enzyme Microb Technol 1999, 24:232-236.
3. Yang P.Y., Wang M.L. Entrapment of microbial cells for wastewater treatment. Wastewater treatment by immobilized bacteria 1990, 45-77. CRC Press. R.D. Tyagi (Ed.).
4. Nakamura H., Shimizu M.S., Karube I. Development of microbial sensors and their application. Adv Biochem Eng Biotechnol 2007, 109:351-394.
5. Chen K.C., Chen J.J., Houng J.Y. Improvement of nitrogen-removal efficiency using immobilized microorganisms with oxidation-reduction potential monitoring. J Ind Microbiol Biotechnol 2000, 25:229-234.
6. Siripattankul S., Wirojangud W., McEvoy J., Khan E. Effect of cell-to-matrix ratio in polyvinyl alcohol immobilized pure and mixed cultures on atrazine degradation. Water Air Soil Pollut 2008, 8:257-266.
7. Chen K.C., Wu J.Y., Huang C.C., Liang Y.M., Hwang S.C. Decolorization of azo dye using PVA immobilized microorganisms. J Biotechnol 2003, 101:241-252.
8. Cassidy M.M., Lee H., Trevors J.T. Environmental applications of immobilized microbial cells: a review. J Ind Microbiol 1996, 16:79-101.
9. Dervakos G.A., Webb C. On the merits of viable-cell immobilization. Biotechnol Adv 1991, 9:559-612.
10. Konsoula Z., Kyriakides M.L. Thermostable α-amylase production by Bacillus subtilis entrapped in calcium alginate gel capsules. Enzyme Microb Technol 2006, 39:690-696.
11. Jen A.C., Wake M.C., Mikos A.G. Review: hydrogels for cell immobilization. Biotechnol Bioeng 1996, 50:357-364.
12. Yang P.Y., Ma T., See T.S., Nitisoravut N. Applying entrapped mixed microbial cell techniques for biological wastewater treatment. Water Sci Technol 1994, 29:487-495.
13. Chen K.C., Lin Y.F. Immobilization of microorganisms with phosphorylated polyvinyl alcohol (PVA) gel. Enzyme Microb Technol 1994, 16:79-83.
14. Kong H.J., Smith M.K., Monney D.J. Designing alginate hydrogels to maintain viability of immobilized cells. Biomaterials 2003, 24:4023-4029.
15. Pramanik S., Khan E. Effects of cell entrapment on growth rate and metabolic activity of mixed cultures in biological wastewater treatment. Enzyme Microb Technol 2008, 43:245-251.
16. Pramanik S., Khan E. Effects of cell entrapment on growth rate and metabolic activity of pure cultures commonly found in biological wastewater treatment. Biochem Eng J 2009, 46:286-293.
17. Keer J.T., Birch L. Molecular methods for the assessment of bacterial viability. J Microbiol Methods 2003, 53:175-183.
18. Paull K.D., Shoemake R.H., Boyd M.R., Parsons J.L., Risbood P.A., Barbera W.A., et al. The synthesis of XTT: a new tetrazolium reagent that is bioreducible to a water-soluble formazan. J Heterocycl Chem 1988, 25:911-914.
19. Roslev P., King G.M. Application of a tetrazolium salt with a water-soluble formazan as an indicator of viability in respiring bacteria. Appl Environ Microbiol 1993, 59:2891-2896.
20. Stevens M.G., Olsen S.C. Comparative analysis of using MTT and XTT in colorimetric assays for quantitating bovine neutrophil bactericidal activity. J Immunol Methods 1993, 157(1-2):225-231.
21. Cerca N., Martins S., Cerca F., Jefferson K.K., Pier G.B., Oliveira R., et al. Comparative assessment of antibiotic susceptibility of coagulase-negative staphylococci in biofilm versus planktonic culture as assessed by bacterial enumeration or rapid XTT colorimetry. J Antimicrob Chemother 2005, 56(2):331-336.
22. De Logu A., Borgna R., Uda P., Sanna A., Pellerano M.L., Saddi B. The 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay as rapid colorimetric method for determination of antibiotic susceptibility of clinical Mycobacterium tuberculosis isolates in liquid medium. Clin Lab 2003, 49(7-8):357-365.
23. Bensaid A., Thierie J., Penninckx M. The use of the tetrazolium salt XTT for the estimation of biological activity of activated sludge cultivated under steady-state and transient regimes. J Microbiol Methods 2000, 40:255-263.
24. Khattak S.F., Spatara M., Roberts S.C. Application of colorimetric assays to assess viability, growth and metabolism of encapsulated cells. Biotechnol Lett 2006, 28(17):1361-1370.
25. Stentelaire C., Antoine N., Cabrol C., Feron G., Durand A. Development of a rapid and highly sensitive biochemical method for the measurement of fungal spore viability. An alternative to the CFU method. Enzyme Microb Technol 2001, 29(8-9):560-566.
26. Kim C.W., Koopman B., Bitton G. INT-dehydrogenase activity test for assessing chlorine and hydrogen peroxide inhibition of filamentous pure cultures and activated sludge. Water Res 1994, 28:1117-1121.
27. Schneider D.A., Gourse R.L. Relationship between growth rate and ATP concentration in Escherichia coli: a bioassay for available cellular ATP. J Biol Chem 2004, 279:8262-8268.
28. Steinberg S.M. A review of environmental applications of bioluminescence measurements. Chemosphere 1995, 30(11):2155-2197.
29. Sule P., Wadhawan T., Carr N.J., Horne S.M., Wolfe A.J., Prüß B.M. A combination of assays reveals biomass differences in biofilms formed by Escherichia coli mutants. Lett Appl Microbiol 2009, 49:299-304.
30. Bartlett R.C., Mazens M., Greenfield B. Acceleration of tetrazolium reduction by bacteria. J Clin Microbiol 1976, 3(3):327-329.
31. Kuehl R.O. Design of experiments: statistical principles of research design and analysis 2000, Brooks/Cole. 2nd ed.
32. Hatzinger P.B., Palmer P., Smith R.L., Penarrieta C.T., Yoshinari T. Applicability of tetrazolium salts for the measurement of respiratory activity and viability of groundwater bacteria. J Microbiol Methods 2003, 52:47-58.
33. McCluskey C., Quinn J.P., McGrath J.W. An evaluation of three new-generation tetrazolium salts for the measurement of respiratory activity in activated sludge microorganisms. Microb Ecol 2005, 49:379-387.
34. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983, 65:55-63.
35. Zimmermann R., Iturriaga R., Birck J.B. Simultaneous determination of the total number of aquatic bacteria and the number thereof involved in respiration. Appl Environ Microbiol 1978, 36:926-935.
36. Shishido M., Toda M. Simulation of oxygen concentration profile in calcium gel beads entrapping microbes during biological phenol degradation. Chem Eng Sci 1996, 51(6):859-872.
37. Chen K.C., Wu J.Y., Yang W.B., Hwang S.C.J. Evaluation of effective diffusion coefficient and intrinsic kinetic parameters on azo dye biodegradation using PVA-immobilized cell beads. Biotechnol Bioeng 2003, 83(7):821-832.
38. Liu J., Zheng Y., Wang W., Wang A. Preparation and swelling properties of semi-IPN hydrogels based on chitosan-g-poly(acrylic acid) and phosphorylated polyvinyl alcohol. J Appl Polym Sci 2009, 114:643-652.
39. Haque H., Russell A.D. Effect of ethylenediaminetetraacetic acid and related chelating agents on whole cells of gram-negative bacteria. Antimicrob Agents Chemother 1974, 5(5):447-452.
40. Groh C.D., MacPherson D.W., Groves D.J. Effect of heat on the sterilization of artificially contaminated water. J Travel Med 1996, 3(1):11-13.
41. Aslani P., Kennedy R.A. Studies on diffusion in alginate gels. I. Effect of cross-linking with calcium or zinc ions on diffusion of acetaminophen. J Control Rel 1996, 42:75-82.
42. Favre E., Leonard M., Laurent A., Dellacherie E. Diffusion of polyethyleneglycos in calcium alginate hydrogels. Colloids Surf A 2001, 194:197-206.
43. Garbayo I., Leon R., Vigara J., Vilchez C. Diffusion characteristics of nitrate and glycerol in alginate. Colloids Surf B 2002, 25:1-9.