Balancing cell migration with matrix degradation enhances gene delivery to cells cultured three-dimensionally within hydrogels

Journal of Controlled Release

Volumn 146, Issue 1, 2010, Pages 128-135

  Shepard, Jaclyn A ^a   Huang, Alyssa ^b   Shikanov, Ariella ^a   Shea, Lonnie D ^a,c  

^a Northwestern University   (United States)

^b NORTHWESTERN UNIVERSITY   (United States)

^c NORTHWESTERN UNIVERSITY   (United States)

Author keywords

Gene delivery; Hydrogel; Migration; Non viral vectors; Three dimensional culture

Indexed keywords

ABSENCE OF CELLS; ARG GLY ASPS; CELL MIGRATION; CROSSLINKED; EXPRESSION LEVELS; FUNDAMENTAL DESIGN; GENE DELIVERY; LIPOPLEXES; MATRIX; MATRIX METALLOPROTEINASES; MECHANISTIC STUDIES; MESH SIZE; NONVIRAL VECTORS; REGENERATIVE MEDICINE; SOLIDS CONTENT; THREE-DIMENSIONAL CULTURE; TISSUE FORMATION; TISSUE REGENERATION; TRANSFECTED CELLS; TRANSIENT EXPRESSION;

ADHESION; CELL ADHESION; CELL CULTURE; CELLS; DEGRADATION; ETHYLENE; ETHYLENE GLYCOL; GENE TRANSFER; NUCLEIC ACIDS; PEPTIDES; POLYETHYLENE GLYCOLS; POLYETHYLENE OXIDES; SOIL MECHANICS; THREE DIMENSIONAL; TISSUE; VECTORS;

HYDROGELS;

ARGINYLGLYCYLASPARTIC ACID; LIPOPLEX; MACROGOL; MATRIX METALLOPROTEINASE;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; CELL ADHESION; CELL MIGRATION; CELL POPULATION; CONTROLLED STUDY; CROSS LINKING; EXTRACELLULAR MATRIX; GENE TARGETING; GENETIC TRANSFECTION; HYDROGEL; NONHUMAN; NONVIRAL GENE DELIVERY SYSTEM; PRIORITY JOURNAL; PROTEIN EXPRESSION; TARGET CELL; THREE DIMENSIONAL IMAGING;

ANIMALS; CELL ADHESION; CELL CULTURE TECHNIQUES; CELL MOVEMENT; CROSS-LINKING REAGENTS; DNA; EXTRACELLULAR MATRIX; GENE TRANSFER TECHNIQUES; HYDROGELS; MATRIX METALLOPROTEINASES; MICE; NIH 3T3 CELLS; OLIGOPEPTIDES; PLASMIDS; POLYETHYLENE GLYCOLS; TISSUE ENGINEERING; TRANSFECTION; TRANSGENES;

EID: 77955266817     PISSN: 01683659     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jconrel.2010.04.032     Document Type: Article

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