Cell-specific extracellular phosphatase activity of dinoflagellate populations in acidified mountain lakes

Journal of Phycology

Volumn 46, Issue 4, 2010, Pages 635-644

  Novotná, Jana ^a,b   Nedbalová, Linda ^a,b   Kopáček, Jiří ^c,d   Vrba, Jaroslav ^c,d  

^a CHARLES UNIVERSITY   (Czech Republic)

^b INSTITUTE OF BOTANY   (Czech Republic)

^c UNIVERSITY OF SOUTH BOHEMIA   (Czech Republic)

^d INSTITUTE OF HYDROBIOLOGY   (Czech Republic)

Author keywords

Acidified lakes; Autecology; ELF97 phosphate; Gymnodinium uberrimum; Peridinium umbonatum; Phytoplankton; Species specific phosphatase activity

Indexed keywords

DINOPHYCEAE; GYMNODINIUM SP.; GYMNODINIUM UBERRIMUM; PERIDINIUM UMBONATUM;

EID: 77955244902     PISSN: 00223646     EISSN: 15298817     Source Type: Journal    
DOI: 10.1111/j.1529-8817.2010.00858.x     Document Type: Article

References (56)

1. Almer B, Dickson W, Ekström C, Hörnström E. Sulphur pollution and the aquatic ecosystems. Sulphur in the Environment, Part II. Ecological Impacts 1978, 271-311. Nriagu J O, Ed, Wiley, New York, pp
2. Bird D F, Kalff J. Algal phagotrophy: regulating factors and importance relative to photosynthesis in Dinobryon (Chrysophyceae). Limnol. Oceanogr. 1987, 32:277-84.
3. Bittl T, Vrba J, Nedoma J, Kopáček J. Impact of ionic aluminium on extracellular phosphatases in acidified lakes. Environ. Microbiol. 2001, 3:578-87.
4. ter Braak C J F, Šmilauer P. CANOCO Reference Manual and Users Guide to Canoco for Windows: Software for Canonical Community Ordination (Version 4.5) 2002, Microcomputer Power, Ithaca, New York, pp
5. Callieri C, Corno G, Bertoni R. Bacterial grazing by mixotrophic flagellates and Daphnia longispina: a comparison in a fishless alpine lake. Aquat. Microb. Ecol. 2006, 42:127-37.
6. Cao X, Štrojsová A, Znachor P, Zapomělová E, Liu G, Vrba J, Zhou Y. Detection of extracellular phosphatases in natural spring phytoplankton of a shallow eutrophic lake (Donghu, China). Eur. J. Phycol. 2005, 40:251-8.
7. Cembella A D, Antia N J, Harrison P J. The utilization of inorganic and organic phosphorus compounds as nutrients by eukaryotic microalgae: a multidisciplinary perspective: Part 1. CRC Crit. Rev. Microbiol. 1984, 11:13-81.
8. Chróst R J. Environmental control of the synthesis and activity of aquatic microbial ectoenzymes. Microbial Enzymes in Aquatic Environments 1991, 29-59. Chróst R J. Ed, Springer, New York, pp
9. Currie D J, Kalff J. A comparison of the abilities of freshwater algae and bacteria to acquire and retain phosphorus. Limnol. Oceanogr. 1984, 29:298-310.
10. Dignum M, Hoogveld H L, Matthijs H C P, Laanbroek H J, Pel R. Detecting the phosphate status of phytoplankton by enzyme-labelled fluorescence and flow cytometry. FEMS Microbiol. Ecol. 2004, 48:29-38.
11. Driscoll C T. A procedure for the fractionation of aqueous aluminum in dilute acidic waters. Int. J. Environ. Anal. Chem. 1984, 16:267-84.
12. Driscoll C T. Aluminium in acidic surface waters: chemistry, transport and effects. Environ. Health Perspect. 1985, 63:93-104.
13. Dyhrman S T, Palenik B. Phosphate stress in cultures and field populations of the dinoflagellate Prorocentrum miniumum detected by a single-cell alkaline phosphatase assay. Appl. Environ. Microbiol. 1999, 65:3205-12.
14. Dyhrman S T, Ruttenberg K C. Presence and regulation of alkaline phosphatase activity in eukaryotic phytoplankton from the coastal ocean: implications for dissolved organic phosphorus remineralization. Limnol. Oceanogr. 2006, 51:1381-90.
15. Dyhrman S T, Webb E A, Anderson D M. Cell-specific detection of phosphorus stress in Trichodesmium from the western North Atlantic. Limnol. Oceanogr. 2002, 47:1832-6.
16. Findlay D L, Kasian S E M, Turner M T, Stainton M P. Responses of phytoplankton and epilithon during acidification and early recovery of a lake. Freshw. Biol. 1999, 42:159-75.
17. Gage M, Gorham E. Alkaline phosphatase activity and cellular phosphorus as an index of the phosphorus status of phytoplankton in Minnesota lakes. Freshw. Biol. 1985, 15:227-33.
18. González-Gil S, Keafer B A, Jovine R V M, Aguilera A, Lu S, Anderson D M. Detection and quantification of alkaline phosphatase in single cells of phosphorus-starved marine phytoplankton. Mar. Ecol. Prog. Ser. 1998, 164:21-35.
19. Healey F P, Hendzel L L. Physiological indicators of nutrient deficiency in lake phytoplankton. Can. J. Fish. Aquat. Sci. 1980, 37:442-53.
20. Hillebrand H, Dürselen C-D, Kirschtel D, Pollingher U, Zohary T. Biovolume calculation for pelagic and benthic microalgae. J. Phycol. 1999, 35:403-24.
21. Hoppe H G. Significance of exoenzymatic activities in the ecology of brackish water: measurements by means of methylumbelliferyl-substrates. Mar. Ecol. Prog. Ser. 1983, 11:299-308.
22. Hörnström E. Phytoplankton in 63 limed lakes in comparison with the distribution in 500 untreated lakes with varying pH. Hydrobiologia 2002, 470:115-26.
23. Hörnström E, Harbom A, Edberg F, Andrén C. The influence of pH on aluminium toxicity in the phytoplankton species Monoraphidium dybowskii and M. griffithii. Water Air Soil Pollut. 1995, 85:817-22.
24. Huang Z, Terpetschnig E, You W, Haugland R P. 2-(2'phosphoryloxyphenyl)-4-(3H)-quinazolinone derivatives as fluorogenic precipitating substrates of phosphatases. Anal. Biochem. 1992, 207:32-9.
25. Jansson M. Induction of high phosphatase activity by aluminium in acidified lakes. Arch. Hydrobiol. 1981, 93:32-44.
26. Jansson M, Olsson H, Pettersson K. Phosphatases; origin, characteristics and function in lakes. Hydrobiologia 1988, 170:157-75.
27. Kalff J. Limnology: Inland Water Ecosystems 2002, Prentice-Hall, Upper Saddle River, New Jersey, pp
28. Kohout L, Fott J. Restoration of zooplankton in a small acidified mountain lake (Plešné Lake, Bohemian Forest) by reintroduction of key species. Biologia 2006, 61:S477-83.
29. Kopáček J, Hejzlar J, Borovec J, Porcal P, Kotorová I. Phosphorus inactivation by aluminium in the water column and sediments: lowering of in-lake phosphorus availability in an acidified watershed-lake ecosystem. Limnol. Oceanogr. 2000, 45:212-25.
30. Kopáček J, Stuchlík E, Veselý J, Schaumburg J, Anderson I C, Fott J, Hejzlar J, Vrba J. Hysteresis in reversal of central European mountain lakes from atmospheric acidification. Water Air Soil Pollut. Focus 2002, 2:91-114.
31. Kopáček J, Turek J, Hejzlar J, Kaňa J, Porcal P. Element fluxes in watershed-lake ecosystems recovering from acidification: I. Čertovo Lake, the Bohemian Forest, 2001-2005. Biologia 2006, 61:S413-26.
32. Kopáček J, Ulrich K U, Hejzlar J, Borovec J, Stuchlík E. Natural inactivation of phosphorus by aluminium in atmospherically acidified water bodies. Water Res. 2001, 35:3783-90.
33. Lorenzen C J. Determination of chlorophyll and phaeopigments: spectrophotometric equations. Limnol. Oceanogr. 1967, 12:2243-6.
34. Model M A, Burkhardt J K. A standard for calibration and shading correction of a fluorescence microscope. Cytometry 2001, 44:309-16.
35. Nedbalová L, Vrba J, Fott J, Kohout L, Kopáček J, Macek M, Soldán T. Biological recovery of the Bohemian Forest lakes from acidification. Biologia 2006, 61:S453-65.
36. Nedbalová L, Vrtiška O. Phytoplankton of Bohemian Forest lakes. Silva Gabreta 2000, 4:213-21.
37. Nedoma J, García J C, Comerma M, Šimek K, Armengol J. Extracellular phosphatases in a Mediterranean reservoir: seasonal, spatial and kinetic heterogeneity. Freshw. Biol. 2006, 51:1264-76.
38. Nedoma J, Štrojsová A, Vrba J, Komárková J, Šimek K. Extracellular phosphatase activity of natural plankton studied with ELF 97 phosphate: fluorescence quantification and labelling kinetics. Environ. Microbiol. 2003, 5:462-72.
39. Nedoma J, Vrba J. Specific activity of cell surface acid phosphatase in different bacterioplankton morphotypes in an acidified mountain lake. Environ. Microbiol. 2006, 8:1271-9.
40. Nedoma J, Vrba J, Hanzl T, Nedbalová L. Quantification of pelagic filamentous microorganisms in aquatic environments. FEMS Microbiol. Ecol. 2001, 38:81-5.
41. Niesel V, Hoehn E, Sudbrack R, Willmitzer H, Chorus I. The occurrence of the dinophyte species Gymnodinium uberrimum and Peridinium willei in German reservoirs. J. Plankton Res. 2007, 29:347-57.
42. Ou L, Huang B, Lin L, Hong H, Zhang F, Chen Z. Phosphorus stress of phytoplankton in the Taiwan Strait determined by bulk and single-cell alkaline phopshatase activity assays. Mar. Ecol. Prog. Ser. 2006, 327:95-106.
43. Rengefors K, Pettersson K, Blenckner T, Anderson D M. Species-specific alkaline phosphatase activity in freshwater spring phytoplankton: application of a novel method. J. Plankton Res. 2001, 23:435-43.
44. Rengefors K, Ruttenberg K C, Haupert C L, Taylor C, Howes B, Anderson D M. Experimental investigation of taxon-specific response of alkaline phosphatase activity in natural freshwater phytoplankton. Limnol. Oceanogr. 2003, 48:1167-75.
45. Reynolds C S. Vegetation Processes in the Pelagic: A Model for Ecosystem Theory 1997, Ecology Institute, Oldendorf/Luhe, Germany, pp
46. Sterner R W, Elser J J. Ecological Stoichiometry: The Biology of Elements from Molecules to the Biosphere 2002, Princeton University Press, Princeton, New Jersey, pp
47. Štrojsová A, Nedoma J, Štrojsová M, Cao X, Vrba J. The role of cell-surface-bound phosphatases in species competition within natural phytoplankton assemblage: an in situ experiment. J. Limnol. 2008, 67:128-38.
48. Štrojsová A, Vrba J. Phytoplankton extracellular phosphatases: investigation using the ELF (enzyme labelled fluorescence) technique. Pol. J. Ecol. 2006, 54:715-23.
49. Štrojsová A, Vrba J. Short term variation in extracellular phosphatase activity: possible limitations for diagnosis of nutrient status in particular algal populations. Aquat. Ecol. 2009, 43:19-25.
50. Štrojsová A, Vrba J, Nedoma J, Komárková J, Znachor P. Seasonal study of extracellular phosphatase expression in the phytoplankton of a eutrophic reservoir. Eur. J. Phycol. 2003, 38:295-306.
51. Štrojsová A, Vrba J, Nedoma J, Šimek K. Extracellular phosphatase activity of freshwater phytoplankton exposed to different in situ phosphorus concentration. Mar. Freshw. Res. 2005, 56:417-24.
52. Veselý J. Investigation of the nature of the Šumava lakes: a review. Čas. Nár. Muz., Řada Přírodověd. 1994, 163:103-20.
53. Vrba J, Kopáček J, Bittl T, Nedoma J, Štrojsová A, Nedbalová L, Kohout L, Fott J. A key role of aluminium in phosphorus availability, food web structure, and plankton dynamics in strongly acidified lakes. Biologia 2006, 61:S441-51.
54. Vrba J, Kopáček J, Fott J, Kohout L, Nedbalová L, Pražáková M, Soldán N, Schaumburg J. Long-term studies (1871-2000) on acidification and recovery of lakes in the Bohemian Forest (central Europe). Sci. Total Environ. 2003a, 310:73-85.
55. Vrba J, Nedoma J, Kohout L, Kopáček J, Nedbalová L, Ráčková P, Šimek K. Massive occurrence of heterotrophic filaments in acidified lakes: seasonal dynamics and composition. FEMS Microbiol. Ecol. 2003b, 46:281-94.
56. Wynne D. The role of phosphatases in the metabolism of Peridinium cinctum, from Lake Kinneret. Hydrobiologia 1981, 83:93-9.