Detection and quantification of alkaline phosphatase in single cells of phosphorus-starved marine phytoplankton

Marine Ecology Progress Series

Volumn 164, Issue , 1998, Pages 21-35

  González Gil, Sonsoles ^a   Keafer, Bruce A ^a   Jovine, Raffael V M ^a   Aguilera, Angeles ^a   Lu, Songhui ^b   Anderson, Donald M ^a  

^a WOODS HOLE OCEANOGRAPHIC INSTITUTION   (United States)

^b UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

Alexandrium fundyense; Alkaline phosphatase; ELF; Phosphorus; Phosphorus limitation; Phytoplankton

Indexed keywords

EID: 2642714903     PISSN: 01718630     EISSN: None     Source Type: Journal    
DOI: 10.3354/meps164021     Document Type: Article

References (57)

1. Anderson DM, Kulis DM, Sullivan JJ, Hall S, Lee C (1990) Dynamics and physiology of saxitoxin production by the dinoflagellates Alexandrium spp. Mar Biol 104(3):511-524
2. Anderson DM, Lindquist NL (1985) Time-course measurements of phosphorus depletion and cyst formation in the dinoflagellate Gonyaulan tamarensis Lebour. J Exp Mar Biol Ecol 86:1-13
3. Arakawa H, Maeda M, Tsuji A (1991) Chemiluminiscent assay of various enzymes using indoxyl derivatives as substrate and its applications to enzyme immunoassay and DNA probe assay. Anal Biochem 199:238-242
4. Berman T (1970) Alkaline phosphatases and phosphorus availability in Lake Kinneret. Limnol Oceanogr 15:663
5. Boni L, Carpene E, Wynne D, Reti M (1989) Alkaline phosphatase activity in Protogonyaulax tamarensis. J Plankton Res 11(5):879-885
6. Brandes D, Elston RN (1956) An electron microscopical study of the histochemical localization of alkaline phosphatase in the cell wall of Chlorella vulgaris. Nature 177:274-275
7. Burnstone MS (1960) Postcoupling, noncoupling and fluorescence techniques for the demonstration of alkaline phosphatase. J Natl Cancer Inst 24:1199-1207
8. Cano RJ, Torres MJ, Klem RE, Palomares JC, Casadeus J (1592) Detection of salmonellas by DNA hybridization with a fluorescent alkaline phosphatase substrate. J Appl Bacteriol 72:393-399
9. Cembella AD, Antia NJ, Harrison PJ (1984) The utilization of inorganic and organic phosphorous compounds as nutrients by eukaryotic microalgae: a multidisciplinary perspective: Part I. CRC Crit Rev Microbiol 10(4):317-391
10. Cotner JB, Wetzel RG (1992) Uptake of dissolved inorganic and organic phosphorus compounds by phytoplankton and bacterioplankton. Limnol Oceanogr 37:232-243
11. Cullen JJ, Yang X, MacIntyre HL (1992) Nutrient limitation of marine photosynthesis. In: Falkowski PG, Woodhead AD (eds) Primary productivity and biogeochemical cycles in the sea. Plenum Press, New York, p 69-88
12. Danks AM, Hammond DN, Wainer BH, Van Buskirk RG, Isaacson RL (1992) Cellular alterations produced by the experimental increase in intracellular calcium and the nature of protective effects from pretreatment with nimopodine. Mol Brain Res 16:168-172
13. Dyhrman ST, Palenik B (1995) Identification and characterization of a Prorocentrum minimum cell surface protein specifically induced under phosphorus limitation. J Phycol 31(3):6
14. Eppley RW (1981) Relationship between nutrient assimilation and growth rate in phytoplankton with a brief view of estimates growth rate in the ocean. In: Platt T (ed) Physiological bases of phytoplankton ecology, Ottawa. Can Bull Fish Aquat Sci 210:251-263
15. Fogg GE (1975) Increase of phytoplankton in temperate waters in the spring. In: Algal cultures and phytoplankton ecology. The University of Wisconsin Press Ltd, Madison, p 84-105
16. Goldman JC, McCarthy JJ, Peavey DG (1979) Growth rate influence on the chemical composition of phytoplankton in oceanic waters. Nature 279:210-215
17. Guibault GG (1990) Fluorescence in enzymology. In: Guibault GG (ed) Practical fluorescence. Marcel Dekker, New York, p 683
18. Guillard RRL, Ryther JH (1962) Studies of marine planktonic diatoms. I. Cycletella nana and Detonula confervacea. Can J Microbiol 8:229-239
19. Haugland RP, Johnson ID (1993) Detecting enzymes in living cells using fluorigenic substrates. J Fluoresc 3(3):119-127
20. Healy FP, Hendzel LL (1979) Fluorimetric measurement of alkaline phosphatase activity in algae. Freshwat Biol 9: 429-439
21. Healy FP, Hendzel LL (1980) Physiological indicators of nutrient deficiency in lake phytoplankton. Can J Fish Aguat Sci 37:442
22. Hill D, Summer GK, Waters MD (1968) An automated fluorimetric assay for alkaline phosphatase using 3-0-methyl-fluorescem phosphate. Anal Biochem 24:9-17
23. Holt SJ (1954) A new approach to the cytochemical localization of enzymes. Proc R Soc Biol Sci 142:160-169
24. Horwitz JP, Chua J, Noel M, Donatti JT, Friesler J (1966) Substrates for cytochemical demonstration of enzyme activity. II. Some dihalo-3-indolyl phosphates and sulfates. J Med Chem 9:447
25. Huang Z, Olson NA, You W, Haugland RP (1992) A sensitive competitive ELISA for 2,4-dinitrophenol using 3,6-fluorescein diphosphate as a fluorigenic substrate. J Immunol Methods 149:261-266
26. Huang Z, You W, Haugland RP, Paragas VB, Olson NA, Haugland RP (1993) A novel fluorigenic substrate for detecting alkaline phosphatase activity in situ. J Histochem Cytochem 41(2):313-317
27. Jackson GA, Williams PM (1985) Importance of dissolved organic nitrogen and phosphorus to biological nutrient cycling. Deep Sea Res 32:223-235
28. Kerkhof L (1992) A comparison of substrates for quantifying the signal from a nonradiolabeled DNA probe. Anal Biochem 205:359-364
29. Kuenzler EJ (1965) Glucose 6-phosphate utilization by marine algae. J Phycol 1:156-164
30. Kuenzler EJ, Perras JP (1965) Phosphatases of marine algae. Biol Bull Mar Biol Lab Woods Hole 128:271-284
31. Larison KD, BreMiller R, Wells KS, Clements I, Haugland RP (1995) Use of a new fluorigenic phosphatase substrate in immunohistochemical applications. J Histochem Cytochem 43(1):77-83
32. La Roche J, Geider RJ, Graziano LM, Murray H, Lewis K (1993) Induction of specific proteins in eukaryotic algae grown under iron-, phosphorus-, or nitrogen-deficient conditions. J Phycol 29:767-777
33. Lien T, Knutsen G (1973) Synchronous cultures of Chlamydomonas reinhardti: properties and regulation of repressible phosphatases. Physiol Plant 28:291-298
34. Matagne RF, Loppes R, Deltour R (1976) Phosphatases of Chlamydomonas reinhardi: biochemical and cytochemical approach with specific mutants. J Bacteriol 126:937
35. McComb AJ, Atkins RP, Birch PB, Gordon DM, Lukatelich RJ (1981) Eutrophication in the Pell-Harvey estuarine system, Western Australia. In: Neilson BJ, Cronin LE (eds) Estuaries and nutrients. Humana Press, Clifton, NJ, p 323-342
36. McGadey J (1970) A tetrazolium method for non-specific alkaline phosphatase. Histochemie 23:180-184
37. Messer G, Ben-Shaul Y (1969) Fine structure of Peridinium westii, a freshwater dinoflagellate. J Protozool 16:272-280
38. Moller M, Myklestad S, Haug A (1975) Alkaline phosphatase of the marine diatoms Chaetoceros affinis vr. willei (Gran.) Hustedt and Skeletonema costatum (Grev.) Cleve. J Exp Mar Biol Ecol 19:217-226
39. Nalewajko C, Lean DRS (1980) Phosphorus. In: Morris I (ed) Studies in ecology, Vol 7, The physiological ecology of phytoplankton. University of California Press, Berkeley, p 235-258
40. Narisawa S, Hoffman MC, Ziomek CA, Millan JL (1992) Embryonic alkaline phosphatase is expressed at M-phase in the spermatogenic lineage of the mouse. Development 116(1):159-165
41. Overbeck J (1962) Untersuchungen zum Phosphathaushalt von Grünalgen. II. Die Verwertung von Pyrophosphat und organisch gebundenen Phosphaten und ihre Beziehung zu den Phosphatasen von Scenedesmus quadricanda. Arch Hydrobiol 58:281-308
42. Parsons TR, Takahashi M, Hargrave B (1984) The primary formation of particulate materials. In: Parsons TR, Takahashi M, Hargrave B (eds) Biological oceanographic processes, 3rd edn. Pergamon Press, New York, p 61-118
43. Patni NJ, Billmire E, Aaronson S (1974) Isolation of the Ochromonas danica plasma membrane and identification of several membrane enzymes. Biochim Biophys Acta 373: 347-355
44. Perry MJ (1972) Alkaline phosphatase activity in subtropical Central North Pacific waters using a sensitive fluorimetric method. Mar Biol 15:113-119
45. Perry MJ (1976) Phosphate utilization by an oceanic diatom in phosphorus-limited chemostat culture and in the oligotrophic waters of the central North Pacific. Limnol Oceanogr 21(1):88-107
46. Price CA (1962) Repression of acid phosphatase synthesis in Euglena gracilis. Science 135:46
47. Rivkin RB, Swift E (1979) Diel and vertical patterns of alkaline phosphatase activity in the dinoflagellate Pyrocystis noctiluca. Limnol Oceanogr 24:107-116
48. Ryther JH, Dunstan WM (1971) Nitrogen, phosphorus and eutrophication in the coastal marine environment. Science 171:1008-1013
49. Schmitter RE, Jurkiewicz AJ (1981) Acid phosphatase localization in PAS-bodies of Gonyaulax. J Cell Sci 51:15-23
50. Shotton DM (1991) Video and opto-digital imaging microscopy. In: Cherry RJ (ed) New techniques of optical microscopy and microspectroscopy. CRC Press, Boca Raton, FL
51. Smith SV (1984) Phosphorus versus nitrogen limitation in the marine environment. Limnol Oceanogr 29(6): 1149-1160
52. Speel EJM, Schutte B, Wiegant J, Ramaekers FCS, Hopman AHN (1992) A novel fluorescence detection method for in situ hybridization, based on the alkaline phosphatase-fast red reaction. J Histochem Cytochem 40(9):1299-1308
53. Thomas WH (1970) On nitrogen deficiency in tropical Pacific oceanic phytoplankton: photosynthetic parameters in poor and rich water. Limnol Oceanogr 15:386-394
54. Uchida T (1992) Alkaline phosphatase and nitrate reductase activities in Prorocentrum micans. Bull Plankton Soc Jpn 38(2):85-92
55. Wynne D (1977) Alterations in activity of phosphatase during the Peridinium bloom in Lake Kinneret. Physiol Plant 40: 219-224
56. Wynne D (1981) The role of phosphatases in the metabolism of Peridinium cinctum from the lake Kinneret. Hydrobiologia 83:93-99
57. Zyomek CA, Lepire ML, Torres I (1990) A highly fluorescent simultaneous azo dye technique for demonstration of non-specific alkaline phosphatase activity. J Histochem Cytochem 38: 437-442