Effects of mouse recombinant bone morphogenetic protein-7 transfection on cell apoptosis, NF-κB, and downstream genes in cultured primary cardiomyocytes after simulated ischemia and reperfusion injury

Journal of Cardiovascular Pharmacology

Volumn 56, Issue 1, 2010, Pages 69-77

  Xu, Ji Hong ^a   Zhao, Yan Yan ^a   Wang, Jun Ke ^a   Yuan, Zhi Gho ^a   Zhang, Tie Zheng ^b  

^a CHINA MEDICAL UNIVERSITY   (China)

^b GENERAL HOSPITAL OF NORTHERN THEATER COMMAND   (China)

Author keywords

apoptosis; bone morphogenetic proteins; Ca2+ overload; NF B; reperfusion injury

Indexed keywords

CALCIUM; COMPLEMENTARY DNA; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INTERCELLULAR ADHESION MOLECULE 1; MALONALDEHYDE; MESSENGER RNA; MONOCYTE CHEMOTACTIC PROTEIN 1; RECOMBINANT OSTEOGENIC PROTEIN 1; SUPEROXIDE DISMUTASE; OSTEOGENIC PROTEIN 1; RECOMBINANT PROTEIN;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL CULTURE; CELL HYPOXIA; CONTROLLED STUDY; DNA VECTOR; ENZYME ACTIVITY; FEMALE; FLUORESCENCE ACTIVATED CELL SORTING; GENE EXPRESSION; GENETIC TRANSFECTION; HEART MUSCLE CELL; HEART MUSCLE ISCHEMIA; HEART PROTECTION; IMMUNOCYTOCHEMISTRY; IN VITRO STUDY; MALE; NEWBORN; NICK END LABELING; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN EXPRESSION; RAT; REOXYGENATION; REPERFUSION INJURY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; WESTERN BLOTTING; ANIMAL; GENETICS; LIPID PEROXIDATION; METABOLISM; MOUSE; MYOCARDIAL REPERFUSION INJURY; PATHOLOGY; PATHOPHYSIOLOGY; WISTAR RAT;

ANIMALS; ANIMALS, NEWBORN; APOPTOSIS; BONE MORPHOGENETIC PROTEIN 7; CALCIUM; CELLS, CULTURED; FEMALE; LIPID PEROXIDATION; MALE; MICE; MYOCARDIAL REPERFUSION INJURY; MYOCYTES, CARDIAC; NF-KAPPA B; RATS; RATS, WISTAR; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; TRANSFECTION;

EID: 77955230469     PISSN: 01602446     EISSN: None     Source Type: Journal    
DOI: 10.1097/FJC.0b013e3181e0badc     Document Type: Article

References (27)

1. Granjeiro JM, Oliveira RC, Bustos-Valenzuela JC, et al. Bone morphogenetic proteins: from structure to clinical use. Braz J Med Biol Res. 2005;38:1463-1473.
2. Hogan BL. Bone morphogenetic proteins: multifunctional regulators of vertebrate development. Genes Dev. 1996;10:1580-1594.
3. Vukicevic S, Basic V, Rogic D, et al. Osteogenic protein-1 (bone morphogenetic protein-7) reduces severity of injury after ischemic acute renal failure in rat. J Clin Invest. 1998;102:202-214.
4. Lin SZ, Hoffer BJ, Kaplan P, et al. Osteogenic protein-1 protects against cerebral infarction induced by MCA ligation in adult rats. Stroke. 1999; 30:126-133.
5. Lefer AM, Tsao PS, Ma XL, et al. Anti-ischaemic and endothelial protective actions of recombinant human osteogenic protein (hOP-1). J Mol Cell Cardiol. 1992;24:585-593.
6. Xu JH, Wang JK, Yuan ZG. Construction of eucaryotic expression plasmid carrying BMP-7 gene and its expression in rat cardiomyocytes. Journal of China Medical University. 2007;36:241-243.
7. Sadoshima J, Jahn L, Takahashi T, et al. Molecular characterization of the stretch-induced adaptation of cultured cardiac cells. J Biol Chem. 1992; 267:10551-10560.
8. Nagy A, Valen G, Ek B, et al. Effects of a novel, low-molecular weight inhibitor of lipid peroxidation on ischemia-reperfusion on injury in isolated rat hearts and in cultured cardiomyocytes. Free Radical Biol Med. 1998;24:1462-1469.
9. Chen XW, Zhang XY, Kubo H, et al. Ca2+ influx-induced sarcoplasmic reticulum Ca2+ overload causes mitochondrial-dependent apoptosis in ventricular myocytes. Circ Res. 2005;97:1009-1017.
10. Cox S, Harvey BK, Sanchez JF, et al. Mediation of BMP-7 neuroprotection by MAPK and PKC in rat primary cortical cultures. Brain Res. 2004;1010:55-61.
11. Xu JH, Wang JK, Yuan ZG, et al. Effects of transfection with bone morphogenetic protein-7 on apoptosis in neonate rate cardiomyocytes induced by hypoxia-reoxygenation. Chinese Journal of Anesthesiology. 2007;27:1114-1117.
12. KirshenbaumLA, Singal PK. Changes in antioxide enzyme in isolated cardiac myocytes subjected to hypoxia-reoxygenation. Lab Invest. 1992;67:796-803.
13. Xie Y, Zhu WZ, Zhu Y, et al. Intermittent high altitude hypoxia protects the heart against lethal Ca2+ overload injury. Life Sci. 2004;76:559-572.
14. Lee MJ, Yang ChW, Jin DCh, et al. Bone morphogenetic protein-7 inhibits constitutive andInterleukin-1-induced monocyte chemoattractant protein-1 expression in human mesangial cells: role for JNK/AP-1 pathway. J Immunol. 2003;170:2557-2563.
15. Gould SE, Day M, Jones SS, et al. BMP-7 regulates chemokine, cytokine, and hemodynamic gene expression in proximal tubule cells. Kidney Int. 2002;61:51-60.
16. Harvey BK, Hoffer BJ, Wang Y. Stroke and TGF-beta proteins: glial cell line-derived neurotrophic factor and bone morphogenetic protein. Pharmacol Ther. 2005;105:113-125.
17. Abraham E. NF-kB activation. Crit Care Med. 2000;28(Suppl): N100-N104.
18. Xu B, Dong GH, Liu H, et al. Recombinant human erythropoietin pretreatment attenuates myocardial infarct size: a possible mechanism involves heat shock protein 70 and attenuation of nuclear factor-kappa B. Ann Clin Lab Sci. 2005;35:161-168.
19. Chandrasekar B, Smith JB, Freeman GL. Ischemia-reperfusion of rat myocardium activates nuclear factor-KB and induces neutrophil infiltration via lipopolysaccharide-induced CXC chemokine. Circulation. 2001;103:2296-2302.
20. Baldwin AS Jr. The NF-kB and I-kB proteins: new discoveries and insights. Ann Rev Immunol. 1996;14:649-681.
21. Im HJ, Pacione C, Chubinskaya S, et al. Inhibitory effects of insulin-like growth factor-1 and osteogenic protein-1 on fibronectin fragment- and interleukin-1beta-stimulated matrix metalloproteinase-13 expression in human chondrocytes. J Biol Chem. 2003;278:25386-25394.
22. Ding M, Zhao GR, Yuan YJ, et al. Aqueous extract of salvia miltiorrhiza regulates adhesion molecule expression of tumor necrosis factor a-induced endothelial cells by blocking activation of nuclear factor kB. J Cardiovasc Pharmacol. 2005;45:516-524.
23. Wang GW, Klein JB, Kang YJ. Metallothionein inhibits doxombicininduced mitochondrial cytochrome C release and caspase-3 activation in cardiomyocytes. J Pharmacol Exp Ther. 2001;298:461-468.
24. Matsui K, Fine A, Zhu B, et al. Identification of two NF-kappa B sites in mouse CD95 ligand (Fas ligand) promoter: functional analysis in T cell hybridoma. J Immumol. 1998;161:3469-3473.
25. Tsai MJ, Weng CF, Shyue SK, et al. Dual effect of adenovirus-mediated transfer of BMP7 in mixed neuron-glial cultures: neuroprotection and cellular differentiation. J Neurosci Res. 2007;85:2950-2959.
26. Xu JH, Wang JK, Yuan ZG, et al. Effects of recombinant human bone morphogenetic protein-7 on activity of NF-kB in myocardium during ischemia-reperfusion in rats. Chinese Journal of Anesthesiology. 2008;28: 916-918.
27. Yue TL, Wang C, Gu JL, et al. Inhibition of extracellular signal-regulated kinase enhances ischemia/reoxygenation-induced apoptosis in cultured cardiac myocytes and exaggerates reperfusion injury in isolated perfused heart. Circ Res. 2000;31:692-699.