Use of EF-Tu mutants for determining and improving aminoacylation efficiency and for purifying aminoacyl tRNAs with non-natural amino acids

Journal of Biochemistry

Volumn 148, Issue 2, 2010, Pages 239-246

  Ohtsuki, Takashi ^a   Yamamoto, Hiromichi ^a   Doi, Yoshio ^a   Sisido, Masahiko ^a  

^a OKAYAMA UNIVERSITY   (Japan)

Author keywords

Aminoacyl RNA Synthesis; Biosynthesis; Elongation Factors; Ribonuclear Protein RNP; Transfer RNA; Translation

Indexed keywords

AMINO ACID; AMINOACYL TRANSFER RNA; ARGININE; ELONGATION FACTOR TU; METIROSINE; SERINE;

AMINOACYLATION; ARTICLE; ESCHERICHIA COLI; IN VITRO STUDY; ISOTOPE LABELING; MUTANT; NONHUMAN; NUCLEOTIDE SEQUENCE; SYNTHESIS;

PEPTIDE ELONGATION FACTOR TU; RNA, TRANSFER, AMINO ACYL; TRANSFER RNA AMINOACYLATION;

ESCHERICHIA COLI;

EID: 77955225547     PISSN: 0021924X     EISSN: 17562651     Source Type: Journal    
DOI: 10.1093/jb/mvq053     Document Type: Article

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