Mitochondrial F1Fo-ATP synthase translocates to cell surface in hepatocytes and has high activity in tumor-like acidic and hypoxic environment

Acta Biochimica et Biophysica Sinica

Volumn 42, Issue 8, 2010, Pages 530-537

  Ma, Zhan ^a   Cao, Manlin ^a   Liu, Yiwen ^a   He, Yiqing ^a   Wang, Yingzhi ^a   Yang, Cuixia ^a   Wang, Wenjuan ^a   Du, Yan ^a   Zhou, Muqing ^a   Gao, Feng ^a  

^a SHANGHAI JIAO TONG UNIVERSITY SCHOOL OF MEDICINE   (China)

Author keywords

ATP synthase; plasma membrane; translocation; tumor marker; tumor like microenvironment

Indexed keywords

ADENOSINE TRIPHOSPHATE; ATP5B PROTEIN, HUMAN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; PROTEIN SUBUNIT; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATE SYNTHASE;

ARTICLE; BIOCATALYSIS; CELL HYPOXIA; CELL LINE; CELL MEMBRANE; CELL STRAIN HEK293; CELL STRAIN HEPG2; CONFOCAL MICROSCOPY; ENZYMOLOGY; FLOW CYTOMETRY; GENETIC TRANSFECTION; GENETICS; HUMAN; LIVER CELL; METABOLISM; MITOCHONDRION; NEOPLASM; PATHOLOGY; PH; PROTEIN TRANSPORT; TUMOR CELL LINE;

ADENOSINE TRIPHOSPHATE; BIOCATALYSIS; CELL HYPOXIA; CELL LINE; CELL LINE, TUMOR; CELL MEMBRANE; FLOW CYTOMETRY; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HEP G2 CELLS; HEPATOCYTES; HUMANS; HYDROGEN-ION CONCENTRATION; MICROSCOPY, CONFOCAL; MITOCHONDRIA; MITOCHONDRIAL PROTON-TRANSLOCATING ATPASES; NEOPLASMS; PROTEIN SUBUNITS; PROTEIN TRANSPORT; RECOMBINANT FUSION PROTEINS; TRANSFECTION;

EID: 77955201072     PISSN: 16729145     EISSN: 17457270     Source Type: Journal    
DOI: 10.1093/abbs/gmq063     Document Type: Article

References (22)

1. Stock D, Leslie AG and Walker JE. Molecular architecture of the rotary motor in ATP synthase. Science 1999, 286: 1700-1705.
2. Chi SL and Pizzo SV. Cell surface F1Fo ATP synthase: a new paradigm? Ann Med 2006, 38: 429-438.
3. Kim BW, Choo HJ, Lee JW, Kim JH and Ko YG. Extracellular ATP is generated by ATP synthase complex in adipocyte lipid rafts. Exp Mol Med 2004, 36: 476-485.
4. Hong D, Jaron D, Buerk DG and Barbee KA. Transport-dependent calcium signaling in spatially segregated cellular caveolar domains. Am J Physiol Cell Physiol 2008, 294: C856-C866.
5. Bae TJ, Kim MS, Kim JW, Kim BW, Choo HJ, Lee JW and Kim KB, et al. Lipid raft proteome reveals ATP synthase complex in the cell surface. Proteomics 2004, 4: 3536-3548.
6. Wang T, Chen Z, Wang X, Shyy JY and Zhu Y. Cholesterol loading increases the translocation of ATP synthase beta chain into membrane caveolae in vascular endothelial cells. Biochim Biophys Acta 2006, 1761: 1182-1190.
7. Champagne E, Martinez LO, Collet X and Barbaras R. Ecto-F1Fo ATP synthase/F1 ATPase: metabolic and immunological functions. Curr Opin Lipidol 2006, 17: 279-284.
8. Ruan Y and Wan M. An optimized procedure for solubilization, reduction, and transfer of human breast cancer membrane-enriched fraction by 2-DE. Electrophoresis 2007, 28: 3333-3340.
9. Huang TC, Chang HY, Hsu CH, Kuo WH, Chang KJ and Juan HF. Targeting therapy for breast carcinoma by ATP synthase inhibitor aurovertin B. J Proteome Res 2008, 7: 1433-1444.
10. Lu ZJ, Song QF, Jiang SS, Song Q, Wang W, Zhang GH and Kan B, et al. Identification of ATP synthase beta subunit (ATPB) on the cell surface as a non-small cell lung cancer (NSCLC) associated antigen. BMC Cancer 2009, 9: 16.
11. Dowling P, Meleady P, Dowd A, Henry M, Glynn S and Clynes M. Proteomic analysis of isolated membrane fractions from superinvasive cancer cells. Biochim Biophys Acta 2007, 1774: 93-101.
12. Chi SL, Wahl ML, Mowery YM, Shan S, Mukhopadhyay S, Hilderbrand SC and Kenan DJ, et al. Angiostatin-like activity of a monoclonal antibody to the catalytic subunit of F1F0 ATP synthase. Cancer Res 2007, 67: 4716-4724.
13. Zhang X, Gao F, Yu LL, Peng Y, Liu HH, Liu JY and Yin M, et al. Dual functions of a monoclonal antibody against cell surface F1F0 ATP synthase on both HUVEC and tumor cells. Acta Pharmacol Sinica 2008, 29: 942-950.
14. Chi SL and Pizzo SV. Angiostatin is directly cytotoxic to tumor cells at low extracellular pH: a mechanism dependent on cell surface-associated ATP synthase. Cancer Res 2006, 66: 875-882.
15. Neckelmann N, Warner CK, Chung A, Kudoh J, Minoshima S, Fukuyama R and Maekawa M, et al. The human ATP synthase beta subunit gene: sequence analysis, chromosome assignment, and differential expression. Genomics 1989, 5: 829-843.
16. Kane LA and Van Eyk JE. Post-translational modifications of ATP synthase in the heart: biology and function. J Bioenerg Biomembr 2009, 41: 145-150.
17. Kim KB, Lee JW, Lee CS, Kim BW, Choo HJ, Jung SY and Chi SG, et al. Oxidation-reduction respiratory chains and ATP synthase complex are localized in detergent-resistant lipid rafts. Proteomics 2006, 6: 2444-2453.
18. Liu C, Chen Z, Chen Z, Zhang T and Lu Y. Multiple tumor types may originate from bone marrow-derived cells. Neoplasia 2006, 8: 716-724.
19. Mangiullo R, Gnoni A, Leone A, Gnoni GV, Papa S and Zanotti F. Structural and functional characterization of FoF1-ATP synthase on the extracellular surface of rat hepatocytes. Biochim Biophys Acta 2008, 1777: 1326-1335.
20. Raghunand N, Gatenby RA and Gillies RJ. Microenvironmental and cellular consequences of altered blood flow in tumours. Br J Radiol 2003, 76: S11-S22.
21. Moser TL, Stack MS, Asplin I, Enghild JJ, Hojrup P, Everitt L and Hubchak S, et al. Angiostatin binds ATP synthase on the surface of human endothelial cells. Proc Natl Acad Sci USA 1999, 96: 2811-2816.
22. Rumjahn SM, Yokdang N, Baldwin KA, Thai J and Buxton IL. Purinergic regulation of vascular endothelial growth factor signaling in angiogenesis. Br J Cancer 2009, 100: 1465-1470.