Simultaneous assay for four bacterial species including alloiococcus otitidis using multiplex-PCR in children with culture negative acute otitis media

Pediatric Infectious Disease Journal

Volumn 29, Issue 8, 2010, Pages 741-745

  Kaur, Ravinder ^a   Adlowitz, Diana G ^a   Casey, Janet R ^b   Zeng, Mingtao ^b   Pichichero, Michael E ^a,b  

^a ROCHESTER GENERAL HOSPITAL   (United States)

^b UNIVERSITY OF ROCHESTER SCHOOL OF MEDICINE AND DENTISTRY   (United States)

Author keywords

acute otitis media; Alloiococcus otitidis; multiplex PCR; otopathogen; Streptococcus pneumoniae

Indexed keywords

BACTERIAL DNA;

ACUTE OTITIS MEDIA; ALLOIOCOCCUS OTITIDIS; ARTICLE; BACTERIAL GROWTH; BACTERIUM CULTURE; BACTERIUM ISOLATION; CHILD; CONTROLLED STUDY; GRAM POSITIVE BACTERIUM; HAEMOPHILUS INFLUENZAE; HUMAN; INFANT; MAJOR CLINICAL STUDY; MORAXELLA CATARRHALIS; MULTIPLEX POLYMERASE CHAIN REACTION; MYRINGOTOMY; NONHUMAN; PRESCHOOL CHILD; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; STREPTOCOCCUS PNEUMONIAE; BACTERIA; BACTERIAL INFECTION; CLASSIFICATION; FEMALE; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; MALE; MICROBIOLOGY; MIDDLE EAR; NASOPHARYNX; OROPHARYNX; OTITIS MEDIA; POLYMERASE CHAIN REACTION; PROCEDURES;

BACTERIA; BACTERIAL INFECTIONS; CHILD, PRESCHOOL; EAR, MIDDLE; FEMALE; HUMANS; INFANT; MALE; NASOPHARYNX; OROPHARYNX; OTITIS MEDIA; POLYMERASE CHAIN REACTION;

EID: 77955171141     PISSN: 08913668     EISSN: 15320987     Source Type: Journal    
DOI: 10.1097/INF.0b013e3181d9e639     Document Type: Article

References (38)

1. Rosenfeld RM, Lous J, Bluestone CD, et al. Recent advances in otitis media. Treatment. Ann Otol Rhinol Laryngol Suppl. 2005;194:114-139.
2. Teele DW, Klein JO, Rosner B. Epidemiology of otitis media during the first seven years of life in children in greater Boston: a prospective, cohort study. J Infect Dis. 1989;160:83-94.
3. Teele DW, Klein JO, Rosner BA. Epidemiology of otitis media in children. Ann Otol Rhinol Laryngol Suppl. 1980;89:5-6.
4. Klein JO BC. Otitis media. In: Feigin RD, ed. Textbook of Pediatric Infectious Diseases. Philadelphia: Saunders. 2004:215-235.
5. Jacobs MR, Dagan R, Appelbaum PC, et al. Prevalence of antimicrobialresistant pathogens in middle ear fluid: multinational study of 917 children with acute otitis media. Antimicrob Agents Chemother. 1998; 42:589-595.
6. Casey JR, Pichichero ME. Changes in frequency and pathogens causing acute otitis media in 1995-2003. Pediatr Infect Dis J. 2004;23:824-828.
7. Pelton SI. Otoscopy for the diagnosis of otitis media. Pediatr Infect Dis J. 1998;17:540-543.
8. Ueyama T, Kurono Y, Shirabe K, et al. High incidence of Haemophilus influenzae in nasopharyngeal secretions and middle ear effusions as detected by PCR. J Clin Microbiol. 1995;33:1835-1838.
9. Hendolin PH, Markkanen A, Ylikoski J, et al. Use of multiplex PCR for simultaneous detection of four bacterial species in middle ear effusions. J Clin Microbiol. 1997;35:2854-2858.
10. Harimaya A, Takada R, Somekawa Y, et al. High frequency of Alloiococcus otitidis in the nasopharynx and in the middle ear cavity of otitis-prone children. Int J Pediatr Otorhinolaryngol. 2006;70:1009-1014.
11. Post JC, White GJ, Aul JJ, et al. Development and validation of a multiplex PCR-based assay for the upper respiratory tract bacterial pathogens Haemophilus influenzae, Streptococcus pneumoniae, and Moraxella catarrhalis. Mol Diagn. 1996;1:29-39.
12. Faden H, Dryja D. Recovery of a unique bacterial organism in human middle ear fluid and its possible role in chronic otitis media. J Clin Microbiol. 1989;27:2488-2491.
13. Ashhurst-Smith C, Hall ST, Walker P, et al. Isolation of Alloiococcus otitidis from indigenous and nonindigenous Australian children with chronic otitis media with effusion. FEMS Immunol Med Microbiol. 2007; 51:163-170.
14. de Miguel Martínez I, Macias AR. Serous otitis media in children: implication of Alloiococcus otitidis. Otol Neurotol. 2008;29:526-530.
15. de Miguel Martínez I, Ramos Macías A, Masgoret Palau E.-Bacterial implication in otitis media with effusion in the childhood-. Acta Otorrinolaringol Esp. 2007;58:408-412.
16. Leskinen K, Hendolin P, Virolainen-Julkunen A, et al. The clinical role of Alloiococcus otitidis in otitis media with effusion. Int J Pediatr Otorhinolaryngol. 2002;66:41-48.
17. Hendolin PH, Karkkainen U, Himi T, et al. High incidence of Alloiococcus otitis in otitis media with effusion. Pediatr Infect Dis J. 1999;18:860-865.
18. Kalcioglu MT, Oncel S, Durmaz R, et al. Bacterial etiology of otitis media with effusion: focusing on the high positivity of Alloiococcus otitidis. New Microbiol. 2002;25:31-35.
19. Durmaz R, Ozerol IH, Kalcioglu MT, et al. Detection of Alloiococcus otitidis in the nasopharynx and in the outer ear canal. New Microbiol. 2002;25:265-268.
20. Beswick AJ, Lawley B, Fraise AP, et al. Detection of Alloiococcus otitis in mixed bacterial populations from middle-ear effusions of patients with otitis media. Lancet. 1999;354:386-389.
21. Frank DN, Spiegelman GB, Davis W, et al. Culture-independent molecular analysis of microbial constituents of the healthy human outer ear. J Clin Microbiol. 2003;41:295-303.
22. Leskinen K, Hendolin P, Virolainen-Julkunen A, et al. Alloiococcus otitidis in acute otitis media. Int J Pediatr Otorhinolaryngol. 2004;68: 51-56.
23. Casey JR, Adlowitz DG, Pichichero ME. New patterns in the otopathogens causing acute otitis media six to eight years after introduction of pneumococcal conjugate vaccine. Pediatr Infect Dis J. [Epub ahead of print]
24. Chomczynski P. A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques. 1993;15:532-537.
25. Lewin BM. Gene Expression. Vol1. London: John Wiley. 1974:285-403.
26. Pichichero ME, Pichichero CL. Persistent acute otitis media: I. Causative pathogens. Pediatr Infect Dis J. 1995;14:178-183.
27. Pichichero ME, Pichichero CL. Persistent acute otitis media. II. Antimicrobial treatment. Pediatr Infect Dis J. 1995;14:183-188.
28. Pichichero ME, McLinn S, Aronovitz G, et al. Cefprozil treatment of persistent and recurrent acute otitis media. Pediatr Infect Dis J. 1997;16: 471-478.
29. Pichichero ME, Casey JR. Emergence of a multiresistant serotype 19A pneumococcal strain not included in the 7-valent conjugate vaccine as an otopathogen in children. JAMA. 2007;298:1772-1778.
30. Pichichero ME, Casey JR, Hoberman A, et al. Pathogens causing recurrent and difficult-to-treat acute otitis media, 2003-2006. Clin Pediatr (Phila). 2008;47:901-906.
31. Kilpi T, Herva E, Kaijalainen T, et al. Bacteriology of acute otitis media in a cohort of Finnish children followed for the first two years of life. Pediatr Infect Dis J. 2001;20:654-662.
32. Leibovitz E, Raiz S, Piglansky L, et al. Resistance pattern of middle ear fluid isolates in acute otitis media recently treated with antibiotics. Pediatr Infect Dis J. 1998;17:463-469.
33. Del Beccaro MA, Mendelman PM, Inglis AF, et al. Bacteriology of acute otitis media: a new perspective. J Pediatr. 1992;120:81-84.
34. Cohen R, de la Rocque F, Boucherat M, et al. Treatment failure in otitis media: an analysis. J Chemother. 1994;6(suppl 4):17-22.
35. Harimaya A, Takada R, Hendolin PH, et al. High incidence of Alloiococcus otitidis in children with otitis media, despite treatment with antibiotics. J Clin Microbiol. 2006;44:946-949.
36. Hall-Stoodley L, Hu FZ, Gieseke A, et al. Direct detection of bacterial biofilms on the middle-ear mucosa of children with chronic otitis media. JAMA. 2006;296:202-211.
37. Post JC, Aul JJ, White GJ, et al. PCR-based detection of bacterial DNA after antimicrobial treatment is indicative of persistent viable bacteria in the chinchilla model of otitis media. Am J Otolaryngol. 1996;17:106-111.
38. Rayner MG, Zhang Y, Gorry MC, et al. Evidence of bacterial metabolic activity in culture-negative otitis media with effusion. JAMA. 1998;279: 296-299.