PCR-based detection of bacterial DNA after antimicrobial treatment is indicative of persistent, viable bacteria in the chinchilla model of otitis media

American Journal of Otolaryngology - Head and Neck Medicine and Surgery

Volumn 17, Issue 2, 1996, Pages 106-111

  Post, J Christopher ^b   Aul, Jerome J ^b   White, Gregory J ^b   Wadowsky, Robert M ^b   Zavoral, Thomas ^b   Tabari, R ^b   Kerber, Brenda ^b   Doyle, William J ^b   Ehrlich, Garth D ^a  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b NONE

Author keywords

[No Author keywords available]

Indexed keywords

AMPICILLIN;

ANIMAL EXPERIMENT; ANTIBIOTIC THERAPY; ARTICLE; BACTERIUM DETECTION; CHINCHILLA; DRUG EFFICACY; HAEMOPHILUS INFLUENZAE; MORAXELLA CATARRHALIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; SECRETORY OTITIS MEDIA; STREPTOCOCCUS PNEUMONIAE; TREATMENT OUTCOME;

EID: 0029962831     PISSN: 01960709     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0196-0709(96)90005-8     Document Type: Article

References (20)

1. Schappert SM: Office Visits for Otitis Media: United States, 1975-1990. U.S. Department of Health and Human Services, Public Health Service, Centers for Disease Control, National Center for Health Statistics.
2. Stool SE, Field MJ: The impact of otitis media. Pediatr Inf Dis J 8:S11-14, 1989 (suppl 1)
3. Bluestone CD, Klein JO: Otitis Media in Infants and Children. Philadelphia, PA, Saunders, 1988
4. Bluestone CD: Otitis media (Burning questions from clinical practice). Otolaryngol Head Neck Surg 106:2-4, 1992
5. Giebink GS: The microbiology of otitis media. Pediatr Infect Dis J 8:S18-S20, 1989 (suppl 1)
6. Post JC, Preston RA, Aul JJ, et al; Molecular analysis of bacterial pathogens in otitis media with effusion. JAMA 273:1598-1604, 1995
7. Hotomi M, Tabata T, Kakiuchi H, et al: Detection of Haemophilus influenzae in middle ear of otitis media with effusion by polymerase chain reaction. International J Ped Otorhinolaryngol 27:119-126, 1993
8. Virolainen A, Salo P, Jero J, et al: Comparison of PCR assay with bacterial culture for detecting Streptococcus pneumoniae in middle ear fluid of children with acute otitis media. J Clin Microbiol 32:1667-1670, 1994
9. Ehrlich GD, Larkins-Pettigrew M, Magit AE, Post JC: Development of PCR-based technologies for the detection of bacteria in middle-ear effusion. Program of the Molecular Biology of Hearing and Deafness (La Jolla). La Jolla, CA: University of California San Diego, 12:1992 (abstr)
10. Ehrlich GD, Aul JJ, Anderson KW, et al: Time Course Comparison of Haemophilus influenzae persistence by culture and PCR in the Chinchilla laniger middle ear following infection and treatment. Program and Abstracts of the 17th Midwinter Meeting of the Association for Research in Otolaryngology (St Petersburg Beach). Des Moines, IA: The Association for Research in Otolaryngology, 158:1994 (abstr)
11. Doyle WJ: Eustachian tube function in the chinchilla. Arch Otolaryngol Head Neck Surg 111:305-308, 1985
12. Doyle WJ, Supance JS, Marshak G, et al: An animal model of acute otitis media consequent to B-lactamaseproducing nontypable Haemophilus influenza. Otolaryngol Head Neck Surg 90:831-836, 1982
13. Giebink GS, Ripley ML, Wright PF: Eustachian tube histopathology during experimental influenza A virus infection in the chinchilla. Ann Otol Rhinol Laryngol 96:199-206, 1987
14. Post JC, Larkins-Pettigrew M, Magit AE, et al: PCR-based strategies for the detection of Haemophilus influenzae and Streptococcus pneumoniae DNA associated with diseases of the ears, nose and throat, in Ehrlich GD, Greenberg SJ (eds): PCR-Based Diagnostics in Infectious Disease. Boston, MA, Blackwell Scientific Publications, 1994, pp 593-608
15. Post JC, White GJ, Aul JJ, et al: Development and validation of a multiplex PCR-based assay for the upper respiratory tract bacterial pathogens, Hemophilus influenzae, Streptococcus pneumoniae, and Moraxella catarrhalis. Mol Diagn (in press)
16. Ehrlich GD, Glaser J, Lavigne K, et al: Prevalence of human T-cell leukemia/lymphoma virus type II infection among high risk individuals: Type-specific identification of HTLVs by polymerase chain reaction. Blood 74:1658-1664, 1989
17. Sirko DA and Ehrlich GD: PCR Laboratory facilities, protocols and operations, in Ehrlich GD, Greenberg SJ (eds): PCR-Based Diagnostics in Infectious Disease. Boston, MA, Blackwell Scientific Publications, 1994, pp 19-44
18. Ehrlich GD, Greenberg S, Abbott M: Detection of human T-cell lymphoma/leukemia viruses (HTLV), in Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds): PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA, 1990, pp 325-336
19. Post JC, Larkins-Pettigrew M, Rydquist-White J, et al: Detection of Haemophilus influenzae DNA in culturally sterile middle-ear effusions by PCR utilizing two primer sets, in Program and Abstracts of the 16th Midwinter Meeting of the Association for Research in Otolaryngology (St Petersburg Beach). Des Moines, IA: The Association for Research in Otolaryngology 72:1993 (abstr)
20. Batschelet E (ed): Introduction to Mathematics for Life Scientists (ed 2). Baltimore, MD, Springer-Verlag, 1976, pp 446-452