A safe and practical procedure for global deprotection of oligoribonucleotides

Journal of Organic Chemistry

Volumn 75, Issue 15, 2010, Pages 5305-5307

  Zewge, Daniel ^a   Gosselin, Francis ^a   Sidler, Rick ^a   Dimichele, Lisa ^a   Cvetovich, Raymond J ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

96-WELL PLATE; DEPROTECTION; LARGE-SCALE PRODUCTION; OLIGORIBONUCLEOTIDES; PRACTICAL PROCEDURES; TERT-BUTYLDIMETHYLSILYL;

ETHERS;

RNA;

2' O TERT BUTYLDIMETHYLSILYL; OLIGORIBONUCLEOTIDE; ORGANOSILICON DERIVATIVE; UNCLASSIFIED DRUG;

AQUEOUS SOLUTION; ARTICLE; CHEMICAL STRUCTURE; COST EFFECTIVENESS ANALYSIS; DEPROTECTION REACTION; LARGE SCALE PRODUCTION; RNA SYNTHESIS;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; FLUORIDES; OLIGORIBONUCLEOTIDES; QUATERNARY AMMONIUM COMPOUNDS; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION; SPECTROPHOTOMETRY, ULTRAVIOLET;

EID: 77955131068     PISSN: 00223263     EISSN: 15206904     Source Type: Journal    
DOI: 10.1021/jo100648e     Document Type: Article

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26. RP and SAX HPLC conditions are described in the Supporting Information. A waterfall chromatogram (SAX HPLC) of purified 21-mer (trityl-off) with three 2′- O -TBS groups is shown (AUC FUFCOMEG OMeGOMeAOMeA OMeAFCFU FUFUFC OMeGOMeGOMeA OMeGOMeUOMeU (a) desilylation with TEA-3HF, pH =11, (b) desilylation with KF, pH = 9
27. 4F added without a buffering acid.