Optimized protocol for detection of HIV-1 drug mutations in patients with low viral load

Journal of Virological Methods

Volumn 168, Issue 1-2, 2010, Pages 152-154

  Stelzl, Evelyn ^a   Troppan, Katharina T ^a   Winkler, Michaela ^a   Korn, Klaus ^b   Kessler, Harald H ^a  

^a MEDICAL UNIVERSITY OF GRAZ   (Austria)

^b UNIVERSITY HOSPITAL ERLANGEN   (Germany)

Author keywords

Drug resistance testing; Human immunodeficiency virus; Sample preparation; Viral load

Indexed keywords

VIRUS RNA; ANTI HUMAN IMMUNODEFICIENCY VIRUS AGENT;

ARTICLE; AUTOMATION; BLOOD SAMPLING; CLINICAL PROTOCOL; CONTROLLED STUDY; DILUTION; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; MUTATIONAL ANALYSIS; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RETROSPECTIVE STUDY; SEQUENCE ANALYSIS; VIRUS DETECTION; VIRUS LOAD; VIRUS TITRATION; ANTIVIRAL RESISTANCE; COMPARATIVE STUDY; DRUG EFFECTS; EVALUATION STUDY; GENETICS; GENOTYPE; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; ISOLATION AND PURIFICATION; MISSENSE MUTATION; PROCEDURES; SENSITIVITY AND SPECIFICITY; VIROLOGY;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1;

ANTI-HIV AGENTS; AUTOMATION; DRUG RESISTANCE, VIRAL; GENOTYPE; HIV INFECTIONS; HIV-1; HUMANS; MUTATION, MISSENSE; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; VIRAL LOAD; VIROLOGY;

EID: 77955094037     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2010.05.007     Document Type: Article

References (7)

1. Aleman S., Soderbarg K., Visco-Comandini U., Sitbon G., Sonnerborg A. Drug resistance at low viraemia in HIV-1-infected patients with antiretroviral combination therapy. Aids 2002, 16:1039-1044.
2. Clumeck N., Pozniak A., Raffi F. European AIDS Clinical Society (EACS) guidelines for the clinical management and treatment of HIV-infected adults. HIV Med. 2008, 9:65-71.
3. Hammer S.M., Eron J.J., Reiss P., Schooley R.T., Thompson M.A., Walmsley S., Cahn P., Fischl M.A., Gatell J.M., Hirsch M.S., Jacobsen D.M., Montaner J.S., Richman D.D., Yeni P.G., Volberding P.A. Antiretroviral treatment of adult HIV infection: 2008 recommendations of the International AIDS Society-USA panel. JAMA 2008, 300:555-570.
4. Kuritzkes D.R., Grant R.M., Feorino P., Griswold M., Hoover M., Young R., Day S., Lloyd R.M., Reid C., Morgan G.F., Winslow D.L. Performance characteristics of the TRUGENE HIV-1 Genotyping Kit and the Opengene DNA sequencing system. J. Clin. Microbiol. 2003, 41:1594-1599.
5. Little S.J., Holte S., Routy J.P., Daar E.S., Markowitz M., Collier A.C., Koup R.A., Mellors J.W., Connick E., Conway B., Kilby M., Wang L., Whitcomb J.M., Hellmann N.S., Richman D.D. Antiretroviral-drug resistance among patients recently infected with HIV. N. Engl. J. Med. 2002, 347:385-394.
6. Parra-Ruiz J., Alvarez M., Chueca N., Pena A., Pasquau J., Lopez-Ruz M.A., Maroto Mdel C., Hernandez-Quero J., Garcia F. [Genotypic resistance in HIV-1-infected patients with persistent low-level viremia]. Enferm. Infecc. Microbiol. Clin. 2009, 27:75-80.
7. Troppan K.T., Stelzl E., Violan D., Winkler M., Kessler H.H. Evaluation of the new VERSANT HIV-1 RNA 1.0 Assay (kPCR) for quantitative detection of human immunodeficiency virus type 1 RNA. J. Clin. Virol. 2009, 46:69-74.