Evaluation of the new VERSANT HIV-1 RNA 1.0 Assay (kPCR) for quantitative detection of human immunodeficiency virus type 1 RNA

Journal of Clinical Virology

Volumn 46, Issue 1, 2009, Pages 69-74

  Troppan, Katharina T ^a   Stelzl, Evelyn ^a   Violan, Deborah ^a   Winkler, Michaela ^a   Kessler, Harald H ^a  

^a MEDICAL UNIVERSITY OF GRAZ   (Austria)

Author keywords

Automation; COBAS AmpliPrep COBAS TaqMan HIV 1 Test, version 2.0; HIV 1 subtype; Human immunodeficiency virus type 1; Real time PCR; VERSANT HIV 1 RNA 1.0 Assay (kPCR)

Indexed keywords

VIRUS RNA;

ARTICLE; BLOOD SAMPLING; CONTROLLED STUDY; CORRELATION ANALYSIS; DIAGNOSTIC PROCEDURE; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; HUMAN IMMUNODEFICIENCY VIRUS INFECTION; MAJOR CLINICAL STUDY; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RNA VIRUS; VIRUS DETECTION; VIRUS LOAD;

HIV-1; HUMANS; REAGENT KITS, DIAGNOSTIC; REPRODUCIBILITY OF RESULTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; VIRAL LOAD;

EID: 67949107943     PISSN: 13866532     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jcv.2009.06.012     Document Type: Article

References (9)

1. Beuselnick K., Ranst M.Y., and Eldere J.Y. Automated extraction of viral-pathogen RNA and DNA for high-throughput quantitative real-time PCR. J Clin Microbiol 43 (2005) 5541-5546
2. Garcia-Diaz A., Clewley G.S., Booth C.L., Labett W., McAllister N., and Geretti A.M. Comparative evaluation of the performance of the Abbott real-time human immunodeficiency virus type 1 (HIV-1) assay for measurement of HIV-1 plasma viral load following automated specimen preparation. J Clin Microbiol 44 (2006) 1788-1791
3. Kwok S., and Sninsky J.J. PCR detection of human immunodeficiency virus type 1 proviral DNA sequences. In: Persing D.H., Smith T.F., Smith F.C., Tenover T.J., and White T.J. (Eds). Diagnostic molecular biology: principles and applications. (1993), ASM Press, Washington, DC
4. Gueudin M., Plantier J.C., Lemee V., Schmitt M.P., Chartier L., Bourlet T., et al. Evaluation of the Roche Cobas TaqMan and Abbott RealTime extraction-quantification systems for HIV-1 subtypes. J Acquir Immune Defic Syndr 44 (2007) 500-505
5. Schneider G.J., Kuper K.G., Abravaya K., Mullen C.R., Schmidt M., Bunse-Grassmann A., et al. Performance evaluation of the QIAGEN EZ1 DSPVirus Kit with Abbott RealTime HIV-1, HBV and HCV assays. J Clin Virol 44 (2009) 292-296
6. Schumacher W., Frick E., Kauselmann M., Maier-Hoyle V., van der Vliet R., and Reiner B. Fully automated quantification of human immunodeficiency virus (HIV) type 1 RNA in human plasma by the COBAS AmpliPrep/COBAS TaqMan system. J Clin Virol 38 (2007) 304-312
7. Schutten M., Fries E., Burghoorn-Maas C., and Niesters H.G.M. Evaluation of the analytical performance of the new Abbott RealTime RT-PCRs for the quantitative detection of HCV and HIV-1 RNA. J Clin Virol 40 (2007) 99-104
8. Sajovitz A.M., Gulamber C., Bozic M., Haas B., Schalk H., Marth E., et al. HIV-1 subtypes and transmission of drug resistance in Southern and Eastern Austria. Clin Chem Lab Med 46 (2008) A61
9. Holguin A., Lopez M., Molinero M., and Soriano V. Performance of Three Commercial Viral Load Assays, Versant Human Immunodeficiency Virus Type 1 (HIV-1) RNA bDNA v3.0, Cobas AmpliPrep/Cobas TaqMan HIV-1, and Nuclisens HIV-1 EasyQ v1.2, Testing HIV-1 Non-B Subtypes and Recombinant Variants. J Clin Microbiol 46 (2008) 2918-2923