Evaluation of small ligand-protein interactions by using T7 RNA polymerase with DNA-modified ligand

Analytical Biochemistry

Volumn 405, Issue 1, 2010, Pages 109-113

  Mie, Masayasu ^a   Sugita, Rie ^a   Endoh, Tamaki ^b   Kobatake, Eiry ^a  

^a TOKYO INSTITUTE OF TECHNOLOGY   (Japan)

^b KONAN UNIVERSITY   (Japan)

Author keywords

DNA tag; Ligand protein interaction; T7 RNA polymerase; Transcription

Indexed keywords

COENZYMES; DNA; RNA; TRANSCRIPTION;

BINDING PROTEINS; COVALENT ATTACHMENT; DIHYDROFOLATE REDUCTASE; LIGAND-PROTEIN INTERACTION; PROOF OF PRINCIPLES; T7 RNA POLYMERASE;

LIGANDS;

BIOTIN; DIHYDROFOLATE REDUCTASE; DNA; METHOTREXATE; RECOMBINANT PROTEIN; RNA POLYMERASE; ANTIBODY; BACTERIOPHAGE T7 RNA POLYMERASE; DNA DIRECTED RNA POLYMERASE; LIGAND; PROTEIN BINDING; VIRUS PROTEIN;

ARTICLE; DNA MODIFICATION; GENETIC TRANSCRIPTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; GENETICS; HUMAN; IMMUNOLOGY; METABOLISM;

ANTIBODIES; BIOTIN; DNA; DNA-DIRECTED RNA POLYMERASES; HUMANS; LIGANDS; PROMOTER REGIONS, GENETIC; PROTEIN BINDING; RECOMBINANT PROTEINS; TETRAHYDROFOLATE DEHYDROGENASE; TRANSCRIPTION, GENETIC; VIRAL PROTEINS;

EID: 77955093518     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2010.06.011     Document Type: Article

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