Fusion of GFP to the M.EcoKI DNA methyltransferase produces a new probe of Type I DNA restriction and modification enzymes

Biochemical and Biophysical Research Communications

Volumn 398, Issue 2, 2010, Pages 254-259

  Chen, Kai ^a   Roberts, Gareth A ^a   Stephanou, Augoustinos S ^a   Cooper, Laurie P ^a   White, John H ^a   Dryden, David T F ^a  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

DNA methyltransferase; DNA restriction modification; Forster resonance energy transfer; Green fluorescent protein; Time resolved fluorescence; Time resolved fluorescence anisotropy

Indexed keywords

DNA METHYLTRANSFERASE; DOUBLE STRANDED DNA; ENDONUCLEASE; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; M.ECOKI DNA METHYLTRANSFERASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; DNA MODIFICATION; DNA RESTRICTION; DNA SEQUENCE; ENZYME MODIFICATION; ENZYME PURIFICATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; IN VIVO STUDY; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS;

DEOXYRIBONUCLEASES, TYPE I SITE-SPECIFIC; DNA; FLUORESCENCE RESONANCE ENERGY TRANSFER; GREEN FLUORESCENT PROTEINS; RECOMBINANT FUSION PROTEINS; SITE-SPECIFIC DNA-METHYLTRANSFERASE (ADENINE-SPECIFIC);

EID: 77955058336     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2010.06.069     Document Type: Article

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