Bluetongue virus RNA detection by RT-qPCR in blood samples of sheep vaccinated with a commercially available inactivated BTV-8 vaccine

Vaccine

Volumn 28, Issue 34, 2010, Pages 5573-5581

  Steinrigl, Adolf ^a   Revilla Fernández, Sandra ^a   Eichinger, Michaela ^a   Koefer, Josef ^a,b   Winter, Petra ^a  

^a AUSTRIAN AGENCY FOR HEALTH AND FOOD SAFETY   (Austria)

^b UNIVERSITY OF VETERINARY MEDICINE   (Austria)

Author keywords

Bluetongue virus; BTV 8 vaccination; RT qPCR

Indexed keywords

BTVPUR AISAP 8; INACTIVATED BTV 8 VACCINE; INACTIVATED VIRUS VACCINE; RNA VACCINE; UNCLASSIFIED DRUG; VIRUS RNA; INACTIVATED VACCINE; VIRUS VACCINE;

ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; BLOOD SAMPLING; BLUETONGUE; BLUETONGUE ORBIVIRUS; CONTROLLED STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; FEMALE; HYPOTHESIS; IMMUNITY; MALE; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; SHEEP; SINGLE DRUG DOSE; VACCINATION; VIRUS DETECTION; VIRUS STRAIN; ANIMAL; BLOOD; IMMUNOLOGY; ISOLATION AND PURIFICATION; PILOT STUDY; VIROLOGY;

ANIMALS; BLUETONGUE; BLUETONGUE VIRUS; FEMALE; MALE; PILOT PROJECTS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, VIRAL; SHEEP; VACCINES, INACTIVATED; VIRAL VACCINES;

EID: 77955053417     PISSN: 0264410X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.vaccine.2010.06.034     Document Type: Article

References (28)

1. Hofmann M.A., Renzullo S., Mader M., Chaignat V., Worwa G., Thuer B. Genetic characterization of Toggenburg orbivirus, a new bluetongue virus, from goats, Switzerland. Emerg Infect Dis 2008, 14:1855-1861.
2. Chaignat V., Worwa G., Scherrer N., Hilbe M., Ehrensperger F., Batten C., et al. Toggenburg orbivirus, a new bluetongue virus: initial detection, first observations in field and experimental infection of goats and sheep. Vet Microbiol 2009, 138:11-19.
3. Huismans H., Erasmus B.J. Identification of the serotype-specific and group-specific antigens of bluetongue virus. Onderstepoort J Vet Res 1981, 48:51-58.
4. Hassan S.S., Roy P. Expression and functional characterization of bluetongue virus VP2 protein: role in cell entry. J Virol 1999, 73:9832-9842.
5. Mayr A., Kaaden O.R. Blauzungenkrankheit. Medizinische Mikrobiologie, Infektions- und Seuchenlehre 2007, 219-221. Stuttgart, Enke Verlag, [in German]. M. Rolle, A. Mayr (Eds.).
6. Schwartz-Cornil I., Mertens P.P., Contreras V., Hemati B., Pascale F., Bréard E., et al. Bluetongue virus: virology, pathogenesis and immunity. Vet Res 2008, 39:46.
7. Elbers A.R., Backx A., Ekker H.M., van der Spek A.N., van Rijn P.A. Performance of clinical signs to detect bluetongue virus serotype 8 outbreaks in cattle and sheep during the 2006-epidemic in The Netherlands. Vet Microbiol 2008, 129:156-162.
8. Maclachlan N.J., Drew C.P., Darpel K.E., Worwa G. The pathology and pathogenesis of bluetongue. J Comp Pathol 2009, 141:1-16.
9. MacLachlan N.J. The pathogenesis and immunology of bluetongue virus infection of ruminants. Comp Immunol Microbiol Infect Dis 1994, 17:197-206.
10. Saegerman C., Berkvens D., Mellor P.S. Bluetongue epidemiology in the European Union. Emerg Infect Dis 2008, 14:539-544.
11. Maan S., Maan N.S., Ross-smith N., Batten C.A., Shaw A.E., Anthony S.J., et al. Sequence analysis of bluetongue virus serotype 8 from the Netherlands 2006 and comparison to other European strains. Virology 2008, 377:308-318.
12. Orrù G., Ferrando M.L., Meloni M., Liciardi M., Savini G., De Santis P. Rapid detection and quantitation of Bluetongue virus (BTV) using a molecular beacon fluorescent probe assay. J Virol Methods 2006, 137:34-42.
13. Shaw A.E., Monaghan P., Alpar H.O., Anthony S., Darpel K.E., Batten C.A., et al. Development and initial evaluation of a RT-qPCR assay to detect bluetongue virus genome segment 1. J Virol Methods 2007, 145:115-126.
14. Toussaint J.F., Sailleau C., Breard E., Zientara S., De Clercq K. Bluetongue virus detection by two real-time RT-qPCRs targeting two different genomic segments. J Virol Methods 2007, 140:115-123.
15. Hofmann M., Griot C., Chaignat V., Perler L., Thür B. Bluetongue disease reaches Switzerland. Schweiz Arch Tierheilkd 2008, 150:49-56. [in German].
16. Vandenbussche F., De Leeuw I., Vandemeulebroucke E., De Clercq K. Emergence of bluetongue serotypes in Europe, part 1: description and validation of four real-time RT-PCR assays for the serotyping of bluetongue viruses BTV-1, BTV-6, BTV-8 and BTV-11. Transbound Emerg Dis 2009, 56(9-10):346-354.
17. Hoffmann B., Eschbaumer M., Beer M. Real-time quantitative reverse transcription-PCR assays specifically detecting bluetongue virus serotypes 1, 6, and 8. J Clin Microbiol 2009, 47:2992-2994.
18. Hoffmann B., Depner K., Schirrmeier H., Beer M. A universal heterologous internal control system for duplex RT-qPCR assays used in a detection system for pestiviruses. J Virol Methods 2006, 136:200-209.
19. Oura C.A., Wood J.L., Sanders A.J., Bin-Tarif A., Henstock M., Edwards L., et al. Seroconversion, neutralising antibodies and protection in bluetongue serotype 8 vaccinated sheep. Vaccine 2009, 27:7326-7330.
20. Eschbaumer M., Hoffmann B., König P., Teifke J.P., Gethmann J.M., Conraths F.J., et al. Efficacy of three inactivated vaccines against bluetongue virus serotype 8 in sheep. Vaccine 2009, 27:4169-4175.
21. Gethmann J., Hüttner K., Heyne H., Probst C., Ziller M., Beer M., et al. Comparative safety study of three inactivated BTV-8 vaccines in sheep and cattle under field conditions. Vaccine 2009, 27:4118-4126.
22. Eschbaumer M., Wäckerlin R., Hoffmann B., Beer M. Re: Detection of bluetongue virus genome after vaccination with an inactivated vaccine (letter to the editor). Vaccine 2009, 28:881-882.
23. Steinrigl A., Eichinger M., Winter P., Revilla-Fernandez S. Bluetongue virus (BTV) RNA is detectable by quantitative RT-PCR in blood samples of sheep vaccinated with a commercial inactivated BTV-8 vaccine. Programme of the 19th annual meeting of the society for virology 2009, [Abstract p. 447].
24. Bustin S.A., Benes V., Garson J.A., Hellemans J., Huggett J., Kubista M., et al. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin Chem 2009, 55:611-622.
25. Bonneau K.R., DeMaula C.D., Mullens B.A., MacLachlan N.J. Duration of viraemia infectious to Culicoides sonorensis in bluetongue virus-infected cattle and sheep. Vet Microbiol 2002, 88:115-125.
26. Judd J.T., Matrone G. Sheep erythrocyte life span in estimation of hemoglobin turnover in iron metabolism studies. J Nutr 1962, 77:264-268.
27. Mertens P.P., Maan N.S., Prasad G., Samuel A.R., Shaw A.E., Potgieter A.C., et al. Design of primers and use of RT-PCR assays for typing European bluetongue virus isolates: differentiation of field and vaccine strains. J Gen Virol 2007, 88:2811-2823.
28. Orrù G., Santis P.D., Solinas F., Savini G., Piras V., Caporale V. Differentiation of Italian field and South African vaccine strains of bluetongue virus serotype 2 using real-time PCR. J Virol Methods 2004, 122:37-43.