Effects of vibrio challenge on digestive gland biomarkers and antioxidant gene expression in Mytilus galloprovincialis

Comparative Biochemistry and Physiology - C Toxicology and Pharmacology

Volumn 152, Issue 3, 2010, Pages 399-406

  Canesi, Laura ^a   Barmo, Cristina ^a   Fabbri, Rita ^a   Ciacci, Caterina ^b   Vergani, Laura ^a   Roch, Philippe ^c   Gallo, Gabriella ^a  

^a UNIVERSITY OF GENOA   (Italy)

^b UNIVERSITY OF URBINO   (Italy)

^c CNRS   (France)

Author keywords

Bivalves; Digestive gland; Expression of antioxidant genes; Lysosomal biomarkers; Oxidative stress; V. anguillarum; V. splendidus

Indexed keywords

ANTIOXIDANT; BIOLOGICAL MARKER; CATALASE; GLUTATHIONE TRANSFERASE; LIPOFUSCIN; METALLOTHIONEIN;

ARTICLE; DOWN REGULATION; ENZYME ACTIVITY; EXOCRINE GLAND; GENE EXPRESSION; IN VIVO STUDY; LYSOSOME MEMBRANE; MYTILUS GALLOPROVINCIALIS; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIBRIO;

BACTERIA (MICROORGANISMS); BIVALVIA; LISTONELLA ANGUILLARUM; MOLLUSCA; MYTILUS; MYTILUS GALLOPROVINCIALIS; VIBRIO; VIBRIO SPLENDIDUS;

EID: 77955049440     PISSN: 15320456     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cbpc.2010.06.008     Document Type: Article

References (52)

1. Allam B., Paillard C., Auffret M., Ford S.E. Effects of the pathogenic Vibrio tapetis on defence factors of susceptible and non-susceptible bivalve species: II. Cellular and biochemical changes following in vivo challenge. Fish Shellfish Immunol. 2006, 20:384-397.
2. Anderson R.S., Patel K.M., Roesijadi G. Oyster metallothionein as an oxyradical scavenger: implications for hemocyte defense responses. Dev. Comp. Immunol. 1999, 23:443-449.
3. Bao Y., Li L., Wu Q., Zhang G. Cloning, characterization, and expression analysis of extracellular copper/zinc superoxide dismutase gene from bay scallop Argopecten irradians. FishShellfish Immunol. 2009, 27:17-25.
4. Baudrimont M., de Montaudouin X., Palvadeau A. Impact of digenean parasite infection on metallothionein synthesis by the cockle (Cerastoderma edule). A multivariate field monitoring. Mar. Pollut. Bull. 2005, 52:494-502.
5. Buico A., Cassino C., Dondero F., Vergani L., Osella D. Radical scavenging abilities of fish MT-A and mussel MT-10 metallothionein isoforms: an ESR study. J. Inorg. Biochem. 2008, 102:921-927.
6. Canesi L., Viarengo A. Age-related differences in glutathione metabolism in mussel tissues (Mytilus edulis L.). Comp. Biochem. Physiol. 1997, 116B:217-221.
7. Canesi L., Gavioli M., Pruzzo C., Gallo G. Bacteria-hemocyte interactions and phagocytosis in marine bivalves. Microsc. Res. Tech. 2002, 57:469-476.
8. Cellura C., Toubiana M., Parrinello N., Roch P. HSP70 gene expression in Mytilus galloprovincialis hemocytes is triggered by moderate heat shock and Vibrio anguillarum, but not by V. splendidus or Micrococcus lysodeikticus. Dev. Comp. Immunol. 2006, 30:984-997.
9. Cellura C., Toubiana M., Parrinello N., Roch P. Specific expression of antimicrobial peptide and HSP70 genes in response to heat-shock and several bacterial challenges in mussels. Fish Shellfish Immunol. 2007, 22:340-350.
10. Ciacci C., Citterio B., Betti M., Canonico B., Roch P., Canesi L. Functional differential immune responses of Mytilus galloprovincialis to bacterial challenge. Comp. Biochem. Physiol. B 2009, 153:365-371.
11. Ciacci, C., Betti, M., Canonico, B., Citterio, B., Roch, P., Canesi, L., in press. Specificity of anti-Vibrio immune response through p38 MAPK and PKC activation in the hemocytes of the mussel Mytilus galloprovincialis, J. Invert. Pathol. doi:10.1016/j.jip.2010.05.010.
12. Costa M.M., Prado-Alvarez M., Gestal C., Li H., Roch P., Novoa B., Figueras A. Functional and molecular immune response of Mediterranean mussel (Mytilus galloprovincialis) haemocytes against pathogen-associated molecular patterns and bacteria. Fish Shellfish Immunol. 2009, 26:515-523.
13. Dagnino A., Allen J.I., Moore M.N., Broeg K., Canesi L., Viarengo A. Development of an expert system for the integration of biomarker responses in mussels into an animal health index. Biomarkers 2007, 12:155-172.
14. + exchanger and PKC. Free Rad. Res. 2005, vol. 39:1059-1070.
15. David E., Tanguy A., Pichavant K., Moraga D. Response of the Pacific oyster Crassostrea gigas to hypoxia exposure under experimental conditions. FEBS J. 2005, 272:5635-5652.
16. 2 oxidative stress and Vibrio alginolyticus bacterial infection. Fish Shellfish Immunol. 2008, 25:446-457.
17. Dimitriadis V.K., Domouhtsidou G.P., Cajaraville M.P. Cytochemical and histochemical aspects of the digestive gland of the mussel Mytilus galloprovincialis (L.) in relation to function. J. Mol. Histol. 2004, 35:501-509.
18. Dondero F., Piacentini L., Banni M., Rebelo M., Burlando B., Viarengo A. Quantitative PCR analysis of two molluscan metallothionein genes unveils differential expression and regulation. Gene 2005, 345:259-270.
19. English T.E., Storey K.B. Freezing and anoxia stresses induce expression of metallothionein in the foot muscle and hepatopancreas of the marine gastropod Littorina littorea. J. Exp. Biol. 2003, 206:2517-2524.
20. Gay M., Berthe F.C.J., Le Roux F. Screening of Vibrio isolates to develop an experimental infection model in the Pacific oyster, Crassostrea gigas. Dis. Aquat. Org. 2004, 59:49-56.
21. Hauton C., Hawkins L.E., Hutchinson S. Response of hemocyte lysosomes to bacterial inoculation in the oysters Ostrea edulis L. and Crassostrea gigas (Thunberg) and the scallop Pecten maximus (L.). Fish Shellfish Immunol. 2001, 11:143-153.
22. Hoarau P., Damiens G., Roméo M., Gnassia-Barelli M., Bebianno M.J. Cloning and expression of a GST-pi gene in Mytilus galloprovincialis. Attempt to use the GST-pi transcript as a biomarker of pollution. Comp. Biochem. Physiol. C 2006, 143:196-203.
23. Huvet A., Herpin A., Degremont L., Lebbreiche Y., Samain J.-F., Cunningham C. The identification of genes from the oyster Crassostrea gigas that are differentially expressed in progeny exhibiting opposed susceptibility to summer mortality. Gene 2004, 343:211-220.
24. Inoue K., Takano H., Shimada A., Wada E., Yanagisawa R., Sakurai M., Satoh M., Yoshikawa T. Role of metallothionein in coagulatory disturbance and systemic inflammation induced by lipopolysaccharide in mice. FASEB J. 2006, 20:533-535.
25. Jenny M.J., Warr G.W., Ringwood A.H., Baltzegar D.A., Chapman R.W. Regulation of metallothionein genes in the American oyster (Crassostrea virginica): ontogeny and differential expression in response to different stressors. Gene 2006, 379:156-165.
26. Kimura T., Itoh N., Takehara M., Oguro I., Ishizaki J.I., Nakanishi T., Tanaka K. Sensitivity of metallothionein-null mice to LPS/D-galactosamine-induced lethality. Biochem. Biophys. Res. Commun. 2001, 280:358-362.
27. Labreuche Y., Lambert C., Soudant P., Boulo V., Huvet A., Nicolas J.L. Cellular and molecular hemocyte responses of the Pacific oyster, Crassostrea gigas, following bacterial infection with Vibrio aestuarianus strain 01/32. Microbes Infect. 2006, 8:2715-2724.
28. Lacoste A., Jalabert F., Malham S., Cueff A., Gelebart F., Cordevant C., Lange M., Poulet S.A. A Vibrio splendidus strain is associated with summer mortality of juvenile oysters Crassostrea gigas in the Bay of Morlaix (North Brittany, France). Dis. Aquat. Org. 2001, 46:139-145.
29. Lambert C., Soudant P., Choquet G., Paillard C. Measurement of Crassostrea gigas hemocyte oxidative metabolism by flow cytometry and the inhibiting capacity of pathogenic vibrios. Fish Shellfish Immunol. 2003, 15:225-240.
30. Lemoine S., Bigot Y., Sellos D., Cosson R.P., Laulier M. Metallothionein isoforms in Mytilus edulis (Mollusca, Bivalvia): complementary DNA characterization and quantification of expression in different organs after exposure to cadmium, zinc, and copper. Mar. Biotechnol. 2000, 2:195-203.
31. Li H., Parisi M.G., Toubiana M., Cammarata M., Roch P. Lysozyme gene expression and hemocyte behaviour in the Mediterranean mussel, Mytilus galloprovincialis, after injection of various bacteria or temperature stresses. Fish Shellfish Immunol. 2008, 25:143-152.
32. Li H., Ni D., Song L., Zhao J., Zhang H., Li L. Molecular cloning and characterization of a catalase gene from Zhikong scallop Chlamys farreri. Fish Shellfish Immunol. 2008, 24:26-34.
33. Liu C.H., Tseng M.C., Cheng W. Identification and cloning of the antioxidant enzyme, glutathione peroxidase, of white shrimp, Litopenaeus vannamei, and its expression following Vibrio alginolyticus infection. Fish Shellfish Immunol. 2007, 23:34-45.
34. Minguez L., Meyer A., Molloy D.P., Giambérini L. Interactions between parasitism and biological responses in zebra mussels (Dreissena polymorpha): importance in ecotoxicological studies. Environ. Res. 2009, 109:843-850.
35. Mitta G., Vandenbulcke F., Hubert F., Roch P. Mussel defensins are synthesised and processed in granulocytes then released into the plasma after bacterial challenge. J. Cell Sci. 1999, 112:4233-4242.
36. Mitta G., Vandenbulcke F., Hubert F., Salzet M., Roch P. Involvement of mytilins in mussel antimicrobial defense. J. Biol. Chem. 2000, 275:12954-12962.
37. Moore M.N. Cytochemical demonstration of latency of lysosomal hydrolases in digestive gland cells of the common mussel Mytilus edulis, and changes induced by thermal stress. Cell Tissue Res. 1976, 175:279-287.
38. Moore M.N. Cytochemical responses of the lysosomal system and NADPH-ferrihemoprotein reductase in molluscan digestive cells to environmental and experimental exposure to xenobiotics. Mar. Ecol. Prog. Ser. 1988, 46:81-89.
39. Moore M.N., Icarus Allen J., McVeigh A. Environmental prognostics: an integrated model supporting lysosomal stress responses as predictive biomarkers of animal health status. Mar. Environ. Res. 2006, 61:278-304.
40. Parisi M.G., Li H., Jouvet L.B.P., Dyrynda E.A., Parinello N., Cammarata M., Roch P. Differential involvement of mussel hemocyte sub-populations in the clearance of bacteria. Fish Shellfish Immunol. 2008, 25:834-840.
41. Perrigault M., Tanguy M., Allam B. Identification and expression of differentially expressed genes in the hard clam, Mercenaria mercenaria, in response to quahog parasite unknown (QPX). BMC Genomics 2009, 10:377. 10.1186/1471-2164-10-377.
42. Pfaffl M.W. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 2001, 29:e45.
43. Piano A., Valbonesi P., Fabbri E. Expression of cytoprotective proteins, heat shock protein 70 and metallothioneins, in tissues of Ostrea edulis exposed to heat and heavy metals. Cell Stress Chaperones 2004, 9:134-142.
44. Prieur D., Mével G., Nicolas J.L., Plusquellec A., Vigneulle M. Interactions between bivalve molluscs and bacteria in the marine environment. Oceanog. Mar. Biol. Annu. Rev. 1990, 28:277-352.
45. Pruzzo C., Gallo G., Canesi L. Persistence of vibrios in marine bivalves: the role of interactions with hemolymph components. Environ. Microbiol. 2005, 7:761-772.
46. Raspor B., Dragun Z., Erk M., Ivankovic D., Pavicic J. Is the digestive gland of Mytilus galloprovincialis a tissue of choice for estimating cadmium exposure by means of metallothioneins?. Sci. Total. Environ. Sci. Total. Environ. 2004, 333:99-108.
47. Sies H. Strategies of antioxidant defenses. Eur. J. Biochem. 1993, 215:213-219.
48. Sigel A., Siegel H., Siegel R.K.O. Metallothioneins and related chelators. Metal Ions in Life Sciences Series 2009, vol. 5. Royal Society of Chemistry, Cambridge, UK, 514 pp.
49. Vergani L., Grattarola M., Borghi C., Dondero F., Viarengo A. Fish and molluscan metallothioneins. FEBS J. 2005, 272:6014-6023.
50. Viarengo A., Canesi L., Pertica M., Livingstone D.R. Seasonal variation of antioxidant defence systems and lipid peroxidation in the digestive gland of mussels. Comp. Biochem. Physiol. 1991, 100C:187-190.
51. Wang L., Song L., Ni D., Zhang H., Liu W. Alteration of metallothionein mRNA in bay scallop Argopecten irradians under cadmium exposure and bacteria challenge. Comp. Biochem. Physiol. C 2009, 149:50-57.
52. Williams H.R., Macey B.M., Burnett L.E., Burnett K.G. Differential localization and bacteriostasis of Vibrio campbellii among tissues of the Eastern oyster, Crassostrea virginica. Dev. Comp. Immunol. 2009, 33:592-600.