Devil's Claw Hairy Root Culture in Flasks and in a 3-L Bioreactor: Bioactive Metabolite Accumulation and Flow Cytometry

Zeitschrift fur Naturforschung - Section C Journal of Biosciences

Volumn 65, Issue 7-8, 2010, Pages 472-478

  Homova, Vediha ^a   Weber, Jost ^b   Schulze, Josef ^b   Bley, Thomas ^b   Alipieva, Kalina ^c   Georgiev, Milen ^d  

^a AGRICULTURAL UNIVERSITY   (Bulgaria)

^b DRESDEN UNIVERSITY OF TECHNOLOGY   (Germany)

^c INSTITUTE OF ORGANIC CHEMISTRY   (Bulgaria)

^d BULGARIAN ACADEMY OF SCIENCES   (Bulgaria)

Author keywords

Hairy Root; Harpagophytum procumbens; Phenylethanoids

Indexed keywords

PLANT DNA;

CYTOLOGY; FLOW CYTOMETRY; GROWTH, DEVELOPMENT AND AGING; HARPAGOPHYTUM; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; KINETICS; METABOLISM; ORGAN SIZE; PLANT ROOT; PROCEDURES; ARTICLE; METHODOLOGY;

CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DNA, PLANT; FLOW CYTOMETRY; HARPAGOPHYTUM; KINETICS; ORGAN SIZE; PLANT ROOTS;

HARPAGOPHYTUM PROCUMBENS;

EID: 77954983181     PISSN: 09395075     EISSN: None     Source Type: Journal    
DOI: 10.1515/znc-2010-7-809     Document Type: Article

References (24)

1. Abou Zid S. and Orihara Y. (2005), Polyacetylenes accumulation in Ambrosia maritima hairy root and cell cultures after elicitation with methyl jasmonate. Plant Cell Tiss. Organ Cult. 81, 65-75.
2. Dembitsky V. M. (2005), Astonishing diversity of natural surfactants: 5. Biologically active glycosides of aromatic metabolites. Lipids 40, 869-900.
3. Dhakulkar S., Ganapathi T. R., Bhargava S., and Bapat V. A. (2005), Induction of hairy roots in Gmelina arborea Roxb. and production of verbascoside in hairy roots. Plant Sci. 169, 812-818.
4. Diaz A. M., Abad M. J., Fernandez L., Silvan A. M., De Santos J., and Bermejo P. (2004), Phenylpropanoid glycosides from Scrophularia scorodonia: in vitro anti-inflammatory activity. Life Sci. 74, 2515-2526.
5. Eibl R. and Eibl D. (2008), Design of bioreactors suitable for plant cell and tissue cultures. Phytochem. Rev. 7, 593-598.
6. Erzos T., Harput U. S., Calis I., and Domnez A. A. (2002), Iridoid, phenylethanoid and monoterpene glycosides from Phlomis sieheana. Turk. J. Chem. 26, 1-8.
7. Fu G., Pang H., and Wong Y. H. (2008), Naturally occurring phenylethanoid glycosides: potential leads for new therapeutics. Curr. Med. Chem. 15, 2592-2613.
8. Georgiev M., Heinrich M., Kerns G., Pavlov A., and Bley Th. (2006), Production of iridoids and phenolics by transformed Harpagophytum procumbens root cultures. Eng. Life Sci. 6, 593-596.
9. Georgiev M., Pavlov A., and Bley Th. (2007), Hairy root type plant in vitro systems as sources of bioactive substances. Appl. Microbiol. Biotechnol. 74, 1175-1185.
10. Georgiev M., Georgiev V., Weber J., Bley Th., Ilieva M., and Pavlov A. (2008), Agrobacterium rhizogenes-mediated genetic transformations: a powerful tool for the production of metabolites. In: Genetically Modified Plants (Wolf T. and Koch J., eds.). Nova Science Publishers Inc., New York, pp. 99-126.
11. Georgiev M., Weber J., and Maciuk A. (2009), Bioprocessing of plant cell cultures for mass production of targeted compounds. Appl. Microbiol. Biotechnol. 83, 809-823.
12. Georgiev M., Alipieva K., Pashova S., Denev P., Angelova M., Kerns G., and Bley Th. (2010), Antioxidant activity of Devil's claw cell biomass and its active constituents. Food Chem. 121, 967-972.
13. Gyurkovska V., Alipieva K., Maciuk A., Dimitrova P., Ivanovska N., Haas C., Bley Th., and Georgiev M. (2010), Anti-inflammatory activity of Devil's claw in vitro systems and their active constituents. Phytomedicine (submitted).
14. Haas C., Weber J., Ludwig-Mueller J., Deponte S., Bley Th., and Georgiev M. (2008), Flow cytometry and phytochemical analysis of a sunflower cell suspension culture in a 5-L bioreactor. Z. Naturforsch. 63 c, 699-705.
15. Loureiro J., Rodriguez E., Dolezel J., and Santos C. (2007), Two new nuclear isolation buffers for plant DNA flow cytometry: a test with 37 species. Ann. Bot. 100, 875-888.
16. Ludwig-Mueller J., Georgiev M., and Bley Th. (2008), Metabolite and hormonal status of hairy root cultures of Devil's claw (Harpagophytum procumbens) in flasks and in a bubble column bioreactor. Process Biochem. 43, 15-23.
17. Miyase T., Koizumi A., Ueno A., Noro T., Kuroyanagi M., Fukushima S., Akiyama Y., and Takemoto T. (1982), Studies on the acyl glycosides from Leucoseptrum japonicum (Miq.) Kitamura et Murata. Chem. Pharm. Bull. 30, 2732-2737.
18. Neumann P., Lysak M., Dolezel J., and Macas J. (1998), Isolation of chromosomes from Pisum sativum L. hairy root cultures and their analysis by flow cytometry. Plant Sci. 137, 205-215.
19. Pettit G. R., Numata A., Takemura T., Ode R. H., Narula A. S., Schmidt J. M., Cragg G. M., and Pase C. P. (1990), Antineoplastic agents, 107. Isolation of acteoside and isoacteoside from Castilleja linariaefolia. J. Nat. Prod. 53, 456-458.
20. Sticher O. and Lahloub M. F. (1982), Phenolic glycosides of Paulownia tomentosa. Planta Med. 46, 145-148.
21. Wysokinska H. and Rozga M. (1998), Establishment of transformed root cultures of Paulownia tomentosa for verbascoside production. J. Plant Physiol. 152, 78-83.
22. Yanpaisan W., King N. J. C., and Doran P. M. (1998), Analysis of cell cycle activity and population dynamics in heterogeneous plant cell suspensions using flow cytometry. Biotechnol. Bioeng. 58, 515-528.
23. Yanpaisan W., King N. J. C., and Doran P. M. (1999), Flow cytometry of plant cells with applications in large-scale bioprocessing. Biotechnol. Adv. 17, 3-27.
24. Zhou Y., Hirotani M., Yoshikawa T., and Furuya T. (1997), Flavonoids and phenylethanoids from hairy root cultures of Scutellaria baicalensis. Phytochemistry 44, 83-87.