Molecular cloning and characterization of a cis-epoxysuccinate hydrolase from Bordetella sp. BK-52

Journal of Microbiology and Biotechnology

Volumn 20, Issue 4, 2010, Pages 659-665

  Pan, Hai Feng ^a   Bao, Wen Na ^b   Xie, Zhi Peng ^a   Zhang, Jian Guo ^a,b   Li, Yongquan ^a  

^a ZHEJIANG UNIVERSITY   (China)

^b Hangzhou Bioking Biochemical Engineering Co Ltd   (China)

Author keywords

Bordetella; Characterization; Cis epoxysuccinate hydrolase; Cloning; D( ) tartaric acid; Purification

Indexed keywords

AMINO ACID; AMMONIUM SULFATE; BACTERIAL ENZYME; BARIUM ION; CALCIUM ION; DITHIOTHREITOL; EDETATE SODIUM; EPOXIDE HYDROLASE; EPOXYSUCCINATE HYDROLASE; SILVER; TARTARIC ACID; UNCLASSIFIED DRUG;

ANION EXCHANGE CHROMATOGRAPHY; ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BIOCATALYST; BORDETELLA; CONTROLLED STUDY; ENANTIOMER; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE OVEREXPRESSION; HIGH TEMPERATURE PROCEDURES; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; PH; PRECIPITATION; SEQUENCE HOMOLOGY; STEREOSPECIFICITY; STRAIN DIFFERENCE; TEMPERATURE SENSITIVITY;

AMINO ACID SEQUENCE; BASE SEQUENCE; BORDETELLA; DNA, BACTERIAL; HYDROLASES; KINETICS; MOLECULAR SEQUENCE DATA; POLYMERASE CHAIN REACTION; STEREOISOMERISM; TARTRATES;

BORDETELLA; BORDETELLA SP.; ESCHERICHIA COLI;

EID: 77954976249     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.0905.05059     Document Type: Article

References (20)

1. Archelas, A. and R. Furstoss. 1998. Epoxide hydrolases: New tools for the synthesis of fine organic chemicals. Trends Biotechnol. 16: 108-116.
2. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
3. Chung, C. T., S. L. Niemela, and R. H. Miller. 1989. One-step preparation of competent Escherichia coli: Transformation and storage of bacterial cells in the same solution. Proc. Natl. Acad. Sci. U.S.A. 86: 2172-2175.
4. Ghosh, A. K., E. S. Koltun, and G. Bilcer. 2001. Tartaric acid and tartarates in the synthesis of bioactive molecules. Synthesis 9: 1281-1301.
5. Huang, T. H. and X. M. Qian. 1990. Production of l (+) tartaric acid. Gong Ye Wei Sheng Wu 6: 14-17.
6. Jacobs, M. H. J., A. J. van den Wijngaard, M. Pentenga, and D. B. Janssen. 1991. Characterization of the epoxide hydrolase from an epichlorohydrin-degrading Pseudomonas sp. Eur. J. Biochem. 202: 1217-1222.
7. Kotik, M. and P. Kyslik. 2006. Purification and characterisation of a novel enantioselective epoxide hydrolase from Aspergillus niger M 200. Biochim. Biophys. Acta 1760: 245-252.
8. Kroutil, W., Y. Genzel, M. Pietzsch, C. Syldatk, and K. Faber. 1998. Purification and characterization of a highly selective epoxide hydrolase from Nocardia sp. EH1. J. Biotechnol. 61: 143-150.
9. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriphage T4. Nature 227: 680-685.
10. Liu, Y. Q. and X. K. Yan. 1983. The colorimetry mensuration for tartaric acid. Gong Ye Wei Sheng Wu 13: 32-37.
11. Liu, Z., Y. Li, Y. Xu, L. Ping, and Y. Zheng. 2007. Cloning, sequencing, and expression of a novel epoxide hydrolase gene from Rhodococcus opacus in Escherichia coli and characterization of enzyme. Appl. Microbiol. Biotechnol. 74: 99-106.
12. Misawa, E., C. K. Chion, I. V. Archer, M. P. Woodland, N. Y. Zhou, S. F. Carter, D. A. Widdowson, and D. J. Leak. 1998. Characterisation of a catabolic epoxide hydrolase from a Corynebacterium sp. Eur. J. Biochem. 253: 173-183.
13. Mischitz, M., K. Faber, and A. Willetts. 1995. Isolation ofa highly enantioselective epoxide hydrolase from Rhodococcus sp. NCIMB 11216. Biotechnol Lett. 17: 893-898.
14. Nakamura, T., T. Nagasawa, F. Yu, I. Watanabe, and H. Yamada. 1994. Purification and characterization of two epoxide hydrolases from Corynebacterium sp. strain N-1074. Appl. Environ. Microbiol. 60: 4630-4633.
15. Nardini, M., R. Rink, D. B. Janssen, and B. W. Dijkstra. 2001. Structure and mechanism of the epoxide hydrolase from Agrobacterium radiobacter AD1. J. Mol. Catal. B Enzym. 11: 1035-1042.
16. Pan, H. F., Z. P. Xie, W. N. Bao, and J. G. Zhang. 2008. Isolation and identification of a novel cis-epoxysuccinate hydrolase-producing Bordetella sp. BK-52 and optimization of enzyme production. Wei Sheng Wu Xue Bao 48: 1075-1081.
17. Pan, H. F., Z. P. Xie, W. N. Bao, and J. G. Zhang. 2008. Optimization of culture conditions to enhance cis-epoxysuccinate hydrolase production in Escherichia coli by response surface methodology. Biochem. Eng. J. 42: 133-138.
18. Rink, R., M. Fennema, M. Smids, U. Dehmel, and D. B. Janssen. 1997. Primary structure and catalytic mechanism of the epoxide hydrolase from Agrobacterium radiobacter AD1. J Biol. Chem. 272: 14650-14657.
19. Steinreiber, A. and K. Faber. 2001. Microbial epoxide hydrolases for preparative biotransformations. Curr. Opin. Biotechnol. 12: 552-558.
20. Willaert, R. and L. De Vuyst. 2006. Continuous production of l(+)-tartaric acid from cis-epoxysuccinate using a membrane recycle reactor. Appl. Microbiol. Biotechnol. 71: 155-163.