Bioscreening of phage display antibody library and expression of a humanized single-chain variable fragment antibody against human connective tissue growth factor (CTGF/CCN2)

Biotechnology and Applied Biochemistry

Volumn 56, Issue 3, 2010, Pages 95-102

  Wu, Guoqiu ^a   Fan, Xiaobo ^b   Wu, Hongbin ^b   Liu, Naifeng ^a   Li, Xiaofang ^b   Gou, Lixia ^a   Nie, Yu ^b   Zhao, Rui ^b   Xi, Tao ^a,b  

^a SOUTHEAST UNIVERSITY   (China)

^b CHINA PHARMACEUTICAL UNIVERSITY   (China)

Author keywords

Connective tissue growth factor (CTGF); Fibrotic disease; Phage display; Single chain variable fragment (scFV); Soluble expression

Indexed keywords

BIOACTIVITY; COLLAGEN; CYTOLOGY; DNA; ENDOTHELIAL CELLS; GELS; GENE ENCODING; MUSCULOSKELETAL SYSTEM; PEPTIDES; PLANTS (BOTANY); PURIFICATION; TISSUE;

CONNECTIVE TISSUE GROWTH FACTOR; CONNECTIVE TISSUE GROWTH FACTOR (CTGF); PHAGE DISPLAY; SINGLE CHAIN VARIABLE FRAGMENTS; SOLUBLE EXPRESSION;

ANTIBODIES;

CONNECTIVE TISSUE GROWTH FACTOR; CONNECTIVE TISSUE GROWTH FACTOR ANTIBODY; HYBRID PROTEIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; THROMBIN; CTGF PROTEIN, HUMAN; PEPTIDE LIBRARY;

ANTIBODY LIBRARY; ANTIBODY SCREENING; ARTICLE; BIOPANNING; CARBOXY TERMINAL SEQUENCE; CELL ASSAY; CELL COUNT; CELL MIGRATION; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA SEQUENCE; ENDOTHELIUM CELL; ENZYME ACTIVE SITE; ENZYME LINKED IMMUNOSORBENT ASSAY; EXPRESSION VECTOR; GEL FILTRATION CHROMATOGRAPHY; HEAVY CHAIN; HUMAN; HUMAN CELL; IMMUNOASSAY; LIGHT CHAIN; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PHAGE DISPLAY; PROTEIN EXPRESSION; PROTEIN PURIFICATION; BIOSYNTHESIS; FIBROSIS; IMMUNOLOGY; PEPTIDE LIBRARY;

CONNECTIVE TISSUE GROWTH FACTOR; FIBROSIS; HUMANS; PEPTIDE LIBRARY; SEQUENCE ANALYSIS, DNA; SINGLE-CHAIN ANTIBODIES;

EID: 77954825373     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: 10.1042/BA20100031     Document Type: Article

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