Extraction and three-phase partitioning behavior of proteases from papaya peels

Process Biochemistry

Volumn 45, Issue 7, 2010, Pages 1172-1175

  Chaiwut, Phanuphong ^a   Pintathong, Punyawatt ^a   Rawdkuen, Saroat ^a  

^a MAE FAH LUANG UNIVERSITY   (Thailand)

Author keywords

Extraction; Papaya peels; Proteases; Three phase partitioning

Indexed keywords

CRUDE EXTRACT; PAPAYA PEELS; PHOSPHATE BUFFERS; PROTEOLYTIC ACTIVITIES; SDS-PAGE; T-BUTANOL; THREE-PHASE PARTITIONING;

ALCOHOLS; OPTIMIZATION; PURIFICATION;

ENZYME ACTIVITY;

EID: 77954818849     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2010.03.019     Document Type: Article

References (19)

1. Chaiwut P., Nitsawang S., Shank L., Kanasawud P. A comparative study on proteolytic components of papaya peel and latex proteases. Chiang Mai J Sci 2007, 34:109-118.
2. Barrett A.J., Rawling N.D. Introduction: cysteine peptidases and their clans. Handbook of proteolytic enzyme 1998, Academic Press, San Diego, pp. 545-66.
3. Caygill J.C. Sulphydryl plant proteases. Enzyme Microb Tech 1979, 1:233-241.
4. Salas C.E., Gomes M.T.R., Hernandez M., Lopes M.T.P. Plant cysteine proteinases: evaluation of the pharmacological activity. Phytochemistry 2008, 69:2263-2269.
5. Pendzhiev A.M. Proteolytic enzymes of papaya: medicinal applications. Pharmaceutic Chem J 2002, 36:315-317.
6. Seki T., Yajima I., Yabu T., Ooguri M., Nakanishi J., Furuya R, et al. Examining an exfoliation-promoting enzyme for cosmetic applications. Biotechnology in cosmetics: concepts, tools and techniques 2007, Allured Pub. Co., Illinois, p. 285-300.
7. Azarkan M., Moussaoui A.E., van Wuytswinkel D., Dehon G., Looze Y. Fractionation and purification of the enzymes stored in the latex of Carica papaya. J Chromatogr B 2003, 790:229-238.
8. Roy I., Gupta M.N. Three-phase affinity partitioning of proteins. Anal Biochem 2002, 11-14.
9. Wati R.K., Theppakorn T., Benjakul S., Rawdkuen S. Three-phase partitioning of trypsin inhibitor from legume seeds. Process Biochem 2009, 44:1307-1314.
10. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72:248-254.
11. Laemmli U.K. Cleavage of structural proteins during assembly of head of bacteriophage T4. Nature 1970, 227:680-685.
12. Garcia-Carreno F.C., Dimes C.E., Haard N.F. Substrate gel electrophoresis for composition and molecular weight of proteinases or proteinaceous proteinase inhibitors. Anal Biochem 1993, 214:65-69.
13. Akin-Osanaiye B.C., Nzelibe H.C., Agbaji A.S. Ethanol production from Carica papaya (pawpaw) fruit waste. Asian J Biochem 2008, 3:188-193.
14. Wall M.M. Ascorbic cid, vitamin A, and mineral composition of banana (Musa sp.) and papaya (Carica papaya) cultivars grown in Hawaii. J Food Compo Anal 2006, 19:434-445.
15. Moussaoui A.E., Nijs M., Paul C., Wintjens R., Vincentelli J., Azarkan M., et al. Revisiting the enzymes stored in the laticifers of Carica papaya in the context of their possible participation in the plant defence mechanism. Cell Mol Life Sci 2001, 58:556-570.
16. Thomas G.E., Rodolfo H.G., Juan M.D., Georgina S.F., Luis C.G., Ingrid R.B., et al. Proteolytic activity in enzymatic extracts from Carica papaya L. cv. Maradol harvest by products. Process Biochem 2009, 44:77-82.
17. Azarkan M., Dibiani R., Baulard C., Baeyens-volant D. Effects of mechanical wounding on Carica papaya cysteine endopeptidase accumulation and activity. Int J Biol Macro 2006, 38:216-224.
18. Dhananjay S.K., Mulimani V.H. Purification of α-galactosidase and invertase by three-phase partitioning from crude extract of Aspergillus oryzae. Biotechnol Lett 2008, 30:1565-1569.
19. Narayan A.V., Madhusudhan M.C., Raghavarao K.S.M.S. Extraction and purification of Ipomoea peroxidase employing three-phase partitioning. Appl Biochem Biotechnol 2008, 151:263-272.