Potential of Aqueous Two-Phase Systems constructed on flexible devices: Human serum albumin as proof of concept

Process Biochemistry

Volumn 45, Issue 7, 2010, Pages 1082-1087

  Garza Madrid, Marcos ^a   Rito Palomares, Marco ^a   Serna Saldívar, Sergio O ^a   Benavides, Jorge ^a  

^a SCHOOL OF ENGINEERING AND SCIENCES   (Mexico)

Author keywords

Aqueous Two Phase Systems; Blood bag; Downstream processing; Flexible devices; Human serum albumin; Recovery and purification

Indexed keywords

AQUEOUS TWO PHASE SYSTEM; AQUEOUS TWO-PHASE SYSTEMS; DOWNSTREAM-PROCESSING; FLEXIBLE DEVICE; HUMAN SERUM ALBUMIN; HUMAN SERUM ALBUMINS;

BLOOD; METABOLITES; POLYETHYLENE GLYCOLS; POLYETHYLENE OXIDES; PROTEINS; RECOVERY;

PURIFICATION;

EID: 77954818445     PISSN: 13595113     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.procbio.2010.03.026     Document Type: Article

References (17)

1. Hundt B., Best C., Schlawin N., Kassner H., Genzel Y., Reichl U. Establishment of a mink enteritis vaccine production process in stirred-tank reactor and wave bioreactor microcarrier culture in 1-10L scale. Vaccine 2007, 25:3987-3995.
2. Kadwell S.H., Hardwicke P.I. Production of baculovirus-expressed recombinant proteins in wave bioreactors. Methods Mol Biol 2007, 388:247-266.
3. Benavides J., Aguilar O., Lapizco-Encinas B.H., Rito-Palomares M. Extraction and purification of bioproducts and nanoparticles using aqueous two-phase systems strategies. Chem Eng Technol 2008, 31:838-845.
4. Salgado J.C., Andrews B.A., Ortuzar M.F., Asenjo J.A. Prediction of the partitioning behaviour of proteins in aqueous two-phase systems using only their amino acid composition. J Chromatogr A 2008, 1178:134-144.
5. Rosa P.A.J., Azevedo A.M., Ferreira I.F., de Vries J., Korporaal R., Verhoef H.J., et al. Affinity partitioning of human antibodies in aqueous two-phase systems. J Chromatogr A 2007, 1162:103-113.
6. Rito-Palomares M. Practical application of aqueous two-phase partition to process development for the recovery of biological products. J Chromatogr B 2004, 807:3-11.
7. Johansson G., Walter H. Partitioning and concentrating biomaterials in aqueous phase systems. Int Rev Cytol 2000, 192:33-60.
8. Zaslavsky B.Y. Aqueous two-phase partitioning; physical chemistry and bioanalytical applications 1995, Mercel Dekker, Inc., New York, pp. 503-667.
9. Gu Z., Glatz C.E. A method for three-dimensional protein characterization and its application to a complex plant (corn) extract. Biotechnol Bioeng 2007, 97:1158-1169.
10. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72:248-254.
11. Huddleston J.G., Ottomar K.W., Ngonyani D.M., Lyddiat A. Influence of system and molecular parameters upon fractionation of intracellular proteins from Saccharomyces by aqueous two-phase partition. Enzyme Microb Technol 1991, 13:24-32.
12. Grossman P.D., Gainer J.L. Correlation of aqueous two-phase partitioning of proteins with changes in free volume. Biotechnol Prog 1988, 4:6-11.
13. Benavides J., Rito-Palomares M. Practical experiences from the development of aqueous two-phase processes for the recovery of high value biological products. J Chem Technol Biotechnol 2008, 83:133-142.
14. Huddleston J., Abelaira J.C., Wang R., Lyddiatt A. Protein partition between the different phases comprising poly(ethylene glycol)-salt aqueous two-phase systems, hydrophobic interaction chromatography and precipitation: a generic description in terms of salting-out effects. J Chromatogr B 1996, 680:31-41.
15. Gianazza E., Frigerio A., Astrua-Testori S., Righetti P.G. The behavior of serum albumin upon isoelectric focusing on immobilized pH gradients. Electrophoresis 1984, 5:310-312.
16. Herosimczyk A., Dejeans N., Sayd T., Ozgo M., Skrzypczak W.F., Mazur A. Plasma proteome analysis: 2D gels and chips. J Physiol Pharmacol 2006, 57(Suppl. 7):81-93.
17. Benavides J., Rito-Palomares M. Simplified two-stage method to B-phycoerythrin recovery from Porphyridium cruentum. J Chromatogr B 2006, 844:39-44.