Preparation and characterization of T7 endonuclease i with single active domain

Progress in Biochemistry and Biophysics

Volumn 37, Issue 4, 2010, Pages 426-432

  Fan, San Hong ^a   Shan, Li Wei ^a   Wang, Bao Li ^a   Guan, Chu Di ^b   Guo, Ai Guang ^a  

^a NORTHWEST A F UNIVERSITY   (China)

^b NEW ENGLAND BIOLABS   (United States)

Author keywords

Catalytic properties; Dimer stability; Domain swapping; Single active domain; T7 endonuclease I

Indexed keywords

ESCHERICHIA COLI;

EID: 77954797034     PISSN: 10003282     EISSN: None     Source Type: Journal    
DOI: 10.3724/SP.J.1206.2009.00580     Document Type: Article

References (12)

1. Declais A C, Lilley D M. New insight into the recognition of branched DNA structure by junction-resolving enzymes. Curr Opin Struct Biol, 2008, 18(1): 86-95
2. Hadden J M, Declais A C, Carr S B, et al. The structural basis of Holliday junction resolution by T7endonuclease I. Nature, 2007, 449(7162): 621-624
3. Hadden J M, Convery M A, Declais A C, et al. Crystal structure of the Holliday junction resolving enzyme T7endonuclease I . Nat Struct Biol, 2001, 8(1): 62-67
4. Declais A C, Fogg J M, Freeman A D, et al. The complex between a four-way DNA junction and T7endonuclease I . EMBO J, 2003, 22(6): 1398-1409
5. Mean R J, Pierides A, Deltas C C, et al. Modification of the enzyme mismatch cleavage method using T7endonuclease I and silver staining. Biotechniques, 2004, 36(5): 758-760
6. Babon J J, McKenzie M, Cotton R G. The use of resolvases T4 endonucleaseVII and T7endonuclease I in mutation detection. Mol Biotechnol, 2003, 23(1): 73-81
7. Guan C, Kumar S, Kucera R, et al. Changing the enzymatic activity of T7endonuclease by mutations at the beta-bridge site: alteration of substrate specificity profile and metal ion requirements by mutation distant from the catalytic domain. Biochemistry, 2004, 43(14): 4313-4322
8. Freeman A D, Declais A C, Lilley D M. Metal ion binding in the active site of the junction-resolving enzyme T7endonuclease I in the presence and in the absence of DNA. J Mol Biol, 2003, 333(1): 59-73
9. Guan C, Kumar S. A single catalytic domain of the junction-resolving enzyme T7endonuclease I is a non-specific nicking endonuclease. Nucleic Acids Res, 2005, 33(19): 6225-6234
10. Guo Z, Eisenberg D. Runaway domain swapping in amyloid-like fibrils of T7endonuclease I . Proc Natl Acad Sci USA, 2006, 103(21): 8042-8047
11. Parkinson M J, Lilley D M. The junction-resolving enzyme T7endonuclease I : quaternary structure and interaction with DNA. J Mol Biol, 1997, 270(2): 169-178
12. Guo Z, Eisenberg D. The mechanism of the amyloidogenic conversion of T7endonuclease I . J Biol Chem, 2007, 282 (20): 14968-14974