Kinase-related protein/telokin inhibits Ca2+-independent contraction in Triton-skinned guinea pig taenia coli

Biochemical Journal

Volumn 429, Issue 2, 2010, Pages 291-302

  Shcherbakova, Olga V ^a   Serebryanaya, Daria V ^a,b   Postnikov, Alexander B ^b   Schroeter, Mechthild M ^c   Zittrich, Stefan ^c   Noegel, Angelika A ^c   Shirinsky, Vladimir P ^a   Vorotnikov, Alexander V ^a,b   Pfitzer, Gabriele ^c  

^a INSTITUTE OF EXPERIMENTAL CARDIOLOGY   (Russian Federation)

^b MOSCOW STATE UNIVERSITY   (Russian Federation)

^c UNIVERSITY OF COLOGNE   (Germany)

Author keywords

Kinase related protein (KRP); Myosin light chain kinase (MLCK); Myosin light chain phosphatase (MLCP); Phosphorylation; Smooth muscle contraction; Telokin

Indexed keywords

CONSTITUTIVELY ACTIVES; GUINEA PIGS; HEAVY MEROMYOSIN; IN-VITRO; MICROCYSTINS; MYOSIN LIGHT CHAIN KINASE; MYOSIN LIGHT CHAIN PHOSPHATASE ( MLCP); MYOSIN LIGHT CHAINS; PHOSPHATASE INHIBITORS; PHOSPHORYLATION SITES; SMOOTH MUSCLE CONTRACTION; SMOOTH MUSCLES;

CALCIUM; MUSCLE; PHOSPHATASES; PROTEINS;

PHOSPHORYLATION;

CALCIUM ION; MICROCYSTIN; MYOSIN HEAVY CHAIN; MYOSIN LIGHT CHAIN; PROTEIN KINASE; TELOKIN PROTEIN; TYLOXAPOL; UNCLASSIFIED DRUG; CALCIUM; CALCIUM BINDING PROTEIN; CYCLIC AMP DEPENDENT PROTEIN KINASE; MICROCYSTIN LR; MITOGEN ACTIVATED PROTEIN KINASE 3; MYLK PROTEIN, HUMAN; MYOSIN LIGHT CHAIN KINASE; MYOSIN SUBFRAGMENT; OCTOXINOL; PEPTIDE FRAGMENT; PRIMER DNA; RECOMBINANT PROTEIN; TELOKIN;

ANIMAL TISSUE; ARTICLE; COLON TENIA; CONTROLLED STUDY; FEMALE; GUINEA PIG; IN VITRO STUDY; MALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN FUNCTION; PROTEIN PHOSPHORYLATION; SMOOTH MUSCLE CONTRACTION; ANIMAL; BINDING SITE; BIOLOGICAL MODEL; CHEMISTRY; CHICKEN; COLON; DRUG EFFECT; GENETICS; HUMAN; METABOLISM; MUSCLE CONTRACTION; NUCLEOTIDE SEQUENCE; PHOSPHORYLATION; PHYSIOLOGY; SMOOTH MUSCLE;

CAVIA; TAENIA;

ANIMALS; BASE SEQUENCE; BINDING SITES; CALCIUM; CALCIUM-BINDING PROTEINS; CHICKENS; COLON; CYCLIC AMP-DEPENDENT PROTEIN KINASES; DNA PRIMERS; FEMALE; GUINEA PIGS; HUMANS; MALE; MICROCYSTINS; MITOGEN-ACTIVATED PROTEIN KINASE 3; MODELS, BIOLOGICAL; MUSCLE CONTRACTION; MUSCLE, SMOOTH; MYOSIN LIGHT CHAINS; MYOSIN SUBFRAGMENTS; MYOSIN-LIGHT-CHAIN KINASE; OCTOXYNOL; PEPTIDE FRAGMENTS; PHOSPHORYLATION; RECOMBINANT PROTEINS;

EID: 77954747598     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20090819     Document Type: Article

References (48)

1. Kamm, K. E. and Stull, J. T. (2001) Dedicated myosin light chain kinases with diverse cellular functions. J. Biol. Chem. 276, 4527-4530
2. Hartshorne, D. J., Ito, M. and Erdodi, F. (2004) Role of protein phosphatase type 1 in contractile functions: myosin phosphatase. J. Biol. Chem. 279, 37211-37214
3. 2+ sensitivity of smooth muscle and nonmuscle myosin II: modulated by G proteins, kinases, and myosin phosphatase. Physiol. Rev. 83, 1325-1358
4. Pfitzer, G. (2001) Regulation of myosin phosphorylation in smooth muscle. J. Appl. Physiol. 91, 497-503
5. 2+-independent phosphorylation of myosin in rat caudal artery and chicken gizzard myofilaments. J. Physiol. 516, 805-824
6. 2+-independent smooth muscle contraction by alternative staurosporine-sensitive kinase. Eur. J. Pharmacol. 376, 315-320
7. Kim, N., Cao, W., Song, I. S., Kim, C. Y., Harnett, K. M., Cheng, L., Walsh, M. P. and Biancani, P. (2004) Distinct kinases are involved in contraction of cat esophageal and lower esophageal sphincter smooth muscles. Am. J. Physiol. Cell Physiol. 287, C384-C394
8. 2+ sensitization in rat ileal longitudinal smooth muscle. Am. J. Physiol. Gastrointest. Liver Physiol. 293, G699-G710
9. Huang, J., Mahavadi, S., Sriwai, W., Hu, W. and Murthy, K. S. (2006) Gi-coupled receptors mediate phosphorylation of CPI-17 and MLC20 via preferential activation of the PI3K/ILK pathway. Biochem. J. 396, 193-200
10. 2+-independent smooth muscle contraction. a novel function for integrin-linked kinase. J. Biol. Chem. 276, 16365-16373
11. Ihara, E. and MacDonald, J. A. (2007) The regulation of smooth muscle contractility by zipper-interacting protein kinase. Can. J. Physiol. Pharmacol. 85, 79-87
12. 2+-free smooth muscle contraction via myosin light chain phosphorylation. J. Biol. Chem. 276, 29567-29574
13. Puetz, S., Lubomirov, L. T. and Pfitzer, G. (2009) Regulation of smooth muscle contraction by small GTPases. Physiology (Bethesda) 24, 342-356
14. 2+ sensitization of contraction in vascular smooth muscle. J. Biol. Chem. 275, 21722-
15. Surks, H. K., Mochizuki, N., Kasai, Y., Georgescu, S. P., Tang, K. M., Ito, M., Lincoln, T. M. and Mendelsohn, M. E. (1999) Regulation of myosin phosphatase by a specific interaction with cGMP- dependent protein kinase Iα. Science 286, 1583-1587
16. 2+ and decreased cGMP-induced relaxation. Proc. Natl. Acad. Sci. U.S.A. 103, 2440-2445
17. Ito, M., Dabrowska, R., Guerriero, Jr, V. and Hartshorne, D. J. (1989) Identification in turkey gizzard of an acidic protein related to the C-terminal portion of smooth muscle myosin light chain kinase. J. Biol. Chem. 264, 13971-13974
18. Collinge, M., Matrisian, P. E., Zimmer, W. E., Shattuck, R. L., Lukas, T. J., Van Eldik, L. J. and Watterson, D. M. (1992) Structure and expression of a calcium-binding protein gene contained within a calmodulin-regulated protein kinase gene. Mol. Cell. Biol. 12, 2359-2371
19. Gallagher, P. J. and Herring, B. P. (1991) The carboxyl terminus of the smooth muscle myosin light chain kinase is expressed as an independent protein, telokin. J. Biol. Chem. 266, 23945-23952
20. Herring, B. P., El-Mounayri, O., Gallagher, P. J., Yin, F. and Zhou, J. (2006) Regulation of myosin light chain kinase and telokin expression in smooth muscle tissues. Am. J. Physiol. Cell Physiol. 291, C817-C827
21. 2+-desensitization through activation of myosin phosphatase in phasic and tonic smooth muscle. J. Muscle Res. Cell Motil. 25, 657-665
22. Krymsky, M. A., Kudryashov, D. S., Shirinsky, V. P., Lukas, T. J., Watterson, D. M. and Vorotnikov, A. V. (2001) Phosphorylation of kinase-related protein (telokin) in tonic and phasic smooth muscles. J. Muscle Res. Cell Motil. 22, 425-437
23. Holden, H. M., Ito, M., Hartshorne, D. J. and Rayment, I. (1992) X-ray structure determination of telokin, the C-terminal domain of myosin light chain kinase, at 2.8 Å resolution. J. Mol. Biol. 227, 840-851
24. Silver, D. L., Vorotnikov, A. V., Watterson, D. M., Shirinsky, V. P. and Sellers, J. R. (1997) Sites of interaction between kinase-related protein and smooth muscle myosin. J. Biol. Chem. 272, 25353-25359
25. Kudryashov, D. S., Vorotnikov, A. V., Dudnakova, T. V., Stepanova, O. V., Lukas, T. J., Sellers, J. R., Watterson, D. M. and Shirinsky, V. P. (2002) Smooth muscle myosin filament assembly under control of a kinase-related protein (KRP) and caldesmon. J. Muscle Res. Cell Motil. 23, 341-351
26. Shirinsky, V. P., Vorotnikov, A. V., Birukov, K. G., Nanaev, A. K., Collinge, M., Lukas, T. J., Sellers, J. R. and Watterson, D. M. (1993) A kinase-related protein stabilizes unphosphorylated smooth muscle myosin minifilaments in the presence of ATP. J. Biol. Chem. 268, 16578-16583
27. Khapchaev, A. Y., Krymsky, M. A., Sidorova, M. V., Bespalova, Zh. D., Wang, C. L., Shirinsky, V. P. and Vorotnikov, A. V. (2004) Novel phosphospecific antibodies for monitoring phosphorylation of proteins encoded by the myosin light chain kinase genetic locus. Biochemistry 69, 789-798
28. MacDonald, J. A., Walker, L. A., Nakamoto, R. K., Gorenne, I., Somlyo, A. V., Somlyo, A. P. and Haystead, T. A. (2000) Phosphorylation of telokin by cyclic nucleotide kinases and the identification of in vivo phosphorylation sites in smooth muscle. FEBS Lett. 479, 83-88 (Pubitemid 30616411)
29. Vorotnikov, A. V., Silver, D. L., Sellers, J. R., Watterson, D. M. and Shirinsky, V. P. (1996) Kinase-related protein is phosphorylated both in vitro and in smooth muscle by mitogen-activated and cyclic AMP-dependent protein kinases. J. Muscle Res. Cell. Motil. 17, 153a
30. 2+-desensitizing effect in smooth muscle. J. Biol. Chem. 276, 24519-24524
31. Sobieszek, A., Andruchov, O. Y., Grabarek, Z., Kulikova, N., Liebetrau, C. and Matusovsky, O. S. (2005) Modulation of myosin filament activation by telokin in smooth muscle liberation of myosin kinase and phosphatase from supramolecular complexes. Biophys. Chem. 113, 25-40
32. Wu, X., Haystead, T. A., Nakamoto, R. K., Somlyo, A. V. and Somlyo, A. P. (1998) Acceleration of myosin light chain dephosphorylation and relaxation of smooth muscle by telokin. Synergism with cyclic nucleotide-activated kinase. J. Biol. Chem. 273, 11362-11369
33. Nieznanski, K. and Sobieszek, A. (1997) Telokin (kinase-related protein) modulates the oligomeric state of smooth-muscle myosin light-chain kinase and its interaction with myosin filaments. Biochem. J. 322, 65-71 (Pubitemid 127732387)
34. Vorotnikov, A. V. (1997) Kinase-related protein: a smooth muscle myosin-binding protein. Int. J. Biochem. Cell Biol. 29, 727-730
35. Hong, F., Haldeman, B. D., John, O. A., Brewer, P. D., Wu, Y. Y., Ni, S., Wilson, D. P., Walsh, M. P., Baker, J. E. and Cremo, C. R. (2009) Characterization of tightly associated smooth muscle myosin-myosin light-chain kinase-calmodulin complexes. J. Mol. Biol. 390, 879-892
36. 2+-independent myosin diphosphorylation and contraction of vascular smooth muscle. Biochem. J. 392, 641-648
37. Adelstein, R. S. and Klee, C. B. (1982) Purification of smooth muscle myosin light-chain kinase. Methods Enzymol. 85, 298-308
38. Sellers, J. R., Pato, M. D. and Adelstein, R. S. (1981) Reversible phosphorylation of smooth muscle myosin, heavy meromyosin, and platelet myosin. J. Biol. Chem. 256, 13137-13142
39. Ikebe, M., Maruta, S. and Reardon, S. (1989) Location of the inhibitory region of smooth muscle myosin light chain kinase. J. Biol. Chem. 264, 6967-6971
40. 2+-induced hydrophobic site on calmodulin: application for purification of calmodulin by phenyl-Sepharose affinity chromatography. Biochem. Biophys. Res. Commun. 104, 830-836
41. Persechini, A., Kamm, K. E. and Stull, J. T. (1986) Different phosphorylated forms of myosin in contracting tracheal smooth muscle. J. Biol. Chem. 261, 6293-6299
42. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685
43. Wirth, A., Schroeter, M., Kock-Hauser, C., Manser, E., Chalovich, J. M., De Lanerolle, P. and Pfitzer, G. (2003) Inhibition of contraction and myosin light chain phosphorylation in guinea-pig smooth muscle by p21-activated kinase 1. J. Physiol. 549, 489-500 (Pubitemid 36701760)
44. Andrews, M. A., Maughan, D. W., Nosek, T. M. and Godt, R. E. (1991) Ion-specific and general ionic effects on contraction of skinned fast-twitch skeletal muscle from the rabbit. J. Gen. Physiol. 98, 1105-1125
45. Gabella, G. (1976) The force generated by a visceral smooth muscle. J. Physiol. 263, 199-213
46. Tokui, T., Ando, S. and Ikebe, M. (1995) Autophosphorylation of smooth muscle myosin light chain kinase at its regulatory domain. Biochemistry 34, 5173-5179
47. 2+, calmodulin and active cAMP-dependent protein kinase. Pflugers Arch. 405, 70-76
48. Cohen, D. M. and Murphy, R. A. (1978) Differences in cellular contractile protein contents among porcine smooth muscles: evidence for variation in the contractile system. J. Gen. Physiol. 72, 369-380