Aspergillus DNA contamination in blood collection tubes

Diagnostic Microbiology and Infectious Disease

Volumn 67, Issue 4, 2010, Pages 392-394

  Harrison, Elizabeth ^a   Stalhberger, Thomas ^a   Whelan, Ruth ^a   Sugrue, Michele ^b   Wingard, John R ^b   Alexander, Barbara D ^c   Follett, Sarah A ^d   Bowyer, Paul ^a   Denning, David W ^a  

^a UNIVERSITY OF MANCHESTER   (United Kingdom)

^b UNIVERSITY OF FLORIDA COLLEGE OF MEDICINE   (United States)

^c DUKE UNIVERSITY MEDICAL CENTER   (United States)

^d Myconostica Ltd   (United Kingdom)

Author keywords

Aspergillus; Blood collection tubes; DNA

Indexed keywords

FUNGAL DNA;

ARTICLE; ASPERGILLUS FUMIGATUS; BLOOD SAMPLING; CONTAINER; FUNGAL CONTAMINATION; NONHUMAN; POLYMERASE CHAIN REACTION; PREVALENCE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; ASPERGILLUS; BLOOD; GENETICS; HUMAN; ISOLATION AND PURIFICATION; LABORATORY DIAGNOSIS; MICROBIOLOGY;

ASPERGILLUS; BLOOD; DNA, FUNGAL; HUMANS; POLYMERASE CHAIN REACTION; SPECIMEN HANDLING;

EID: 77954737369     PISSN: 07328893     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.diagmicrobio.2010.02.028     Document Type: Article

References (10)

1. Challier S., Boyer S., Abachin E., Berche P. Development of a serum-based Taqman real-time PCR assay for diagnosis of invasive aspergillosis. J. Clin. Microbiol. 2004, 42(2):844-846.
2. Chen S.C., Halliday C.L., Meyer W. A review of nucleic acid-based diagnostic tests for systemic mycoses with an emphasis on polymerase chain reaction-based assays. Med. Mycol. 2002, 40:333-357.
3. Denning D.W. Invasive aspergillosis. Clin. Infect. Dis. 1998, 26:781-803.
4. Herrera M.L., Vallor A.C., Gelfond J.A., Patterson T.F., Wickes B.L. Strain-dependent variation in 18S ribosomal DNA Copy numbers in Aspergillus fumigatus. J. Clin. Microb. 2009, 47(5):1325-1332.
5. Loeffler J., Hebart H., Bialek R., Hagmeyer L., Schmidt D., Serey F.P., Hartmann M., Eucker J., Einsele H. Contaminations occurring in fungal PCR assays. J. App. Microbiol. 1999, 37(4):1200-1202.
6. Maschmeyer G., Haas A. The epidemiology and treatment of infections in cancer patients. Int. J. Antimicrob. Agents 2008, 31:193-197.
7. Menzin J., Meyers J.L., Friedman M., Perfect J.R., Langston A.A., Danna R.P., Papadopoulos G. Mortality, length of hospitalization, and costs associated with invasive fungal infections in high-risk patients. Am. J. Health Syst. Pharm. 2009, 66:1711-1717.
8. Palmer J., Francesconi A., Kasai M., Walsh T.J., Orle K. Sources of false positive Aspergillus DNA by PCR from normal human blood 2001.
9. Perlin D.S., Zhao Y. Molecular diagnostic platforms for detecting Aspergillus. Med. Mycol. 2009, 47(Suppl 1):S223-S232.
10. Tyagi S., Kramer F.R. Molecular beacons: probes that fluoresce upon hybridization. Nat Biotechnol 1996, 14:303-308.