Complex phenotypes of a mutant inactivated for CymR, the global regulator of cysteine metabolism in Bacillus subtilis

FEMS Microbiology Letters

Volumn 309, Issue 2, 2010, Pages 201-207

  Hullo, Marie Françoise ^a   Martin Verstraete, Isabelle ^a,b   Soutourina, Olga ^a,b  

^a INSTITUT PASTEUR   (France)

^b UNIVERSITÉ PARIS DESCARTES   (France)

Author keywords

cysteine; cysteine metabolism regulator; disulfide stress; oxidative stress; tellurite

Indexed keywords

BRANCHED CHAIN AMINO ACID; CYSTEINE; DISULFIDE; HYDROGEN PEROXIDE; HYDROGEN SULFIDE; PARAQUAT; PROTEIN CYMR; REPRESSOR PROTEIN; TELLURITE; TELLURIUM DERIVATIVE; UNCLASSIFIED DRUG; BACTERIAL PROTEIN;

AMINO ACID METABOLISM; ARTICLE; BACILLUS SUBTILIS; BACTERIAL GROWTH; BACTERIUM MUTANT; GENE DELETION; GENE INACTIVATION; METABOLITE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; GENE EXPRESSION REGULATION; GENE SILENCING; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; MUTATION; REGULATOR GENE;

BACILLUS SUBTILIS;

BACILLUS SUBTILIS; BACTERIAL PROTEINS; CYSTEINE; GENE EXPRESSION REGULATION, BACTERIAL; GENE SILENCING; GENES, REGULATOR; MUTATION; PHENOTYPE;

EID: 77954702629     PISSN: 03781097     EISSN: 15746968     Source Type: Journal    
DOI: 10.1111/j.1574-6968.2010.02043.x     Document Type: Article

References (31)

1. Albanesi D, Mansilla MC, Schujman GE de Mendoza D (2005) Bacillus subtilis cysteine synthetase is a global regulator of the expression of genes involved in sulfur assimilation. J Bacteriol 187 : 7631 7638.
2. Almeida CE, Felicio DL, Galhardo RS, Cabral-Neto JB Leitao AC (1999) Synergistic lethal effect between hydrogen peroxide and neocuproine (2,9-dimethyl 1,10-phenanthroline) in Escherichia coli. Mutat Res 433 : 59 66.
3. Auger S, Gomez MP, Danchin A Martin-Verstraete I (2005) The PatB protein of Bacillus subtilis is a C-S-lyase. Biochimie 87 : 231 238.
4. Burguière P, Auger S, Hullo MF, Danchin A Martin-Verstraete I (2004) Three different systems participate in l-cystine uptake in Bacillus subtilis. J Bacteriol 186 : 4875 4884.
5. Burguière P, Fert J, Guillouard I, Auger S, Danchin A Martin-Verstraete I (2005) Regulation of the Bacillus subtilis ytmI operon, involved in sulfur metabolism. J Bacteriol 187 : 6019 6030.
6. Chasteen TG, Fuentes DE, Tantalean JC Vasquez CC (2009) Tellurite: history, oxidative stress, and molecular mechanisms of resistance. FEMS Microbiol Rev 33 : 820 832.
7. Choi SY, Reyes D, Leelakriangsak M Zuber P (2006) The global regulator Spx functions in the control of organosulfur metabolism in Bacillus subtilis. J Bacteriol 188 : 5741 5751.
8. Conway ME, Yennawar N, Wallin R, Poole LB Hutson SM (2003) Human mitochondrial branched chain aminotransferase: structural basis for substrate specificity and role of redox active cysteines. Biochim Biophys Acta 1647 : 61 65.
9. Cowman RA, Baron SS Fitzgerald RJ (1983) Cysteine toxicity for oral streptococci and effect of branched-chain amino acids. Infect Immun 39 : 1107 1113.
10. del Castillo Lozano ML, Tache R, Bonnarme P Landaud S (2007) Evaluation of a quantitative screening method for hydrogen sulfide production by cheese-ripening microorganisms: the first step towards l-cysteine catabolism. J Microbiol Meth 69 : 70 77.
11. Even S, Burguière P, Auger S, Soutourina O, Danchin A Martin-Verstraete I (2006) Global control of cysteine metabolism by CymR in Bacillus subtilis. J Bacteriol 188 : 2184 2197.
12. Harris CL (1981) Cysteine and growth inhibition of Escherichia coli: threonine deaminase as the target enzyme. J Bacteriol 145 : 1031 1035.
13. Hochgrafe F, Mostertz J, Pother DC, Becher D, Helmann JD Hecker M (2007) S-Cysteinylation is a general mechanism for thiol protection of Bacillus subtilis proteins after oxidative stress. J Biol Chem 282 : 25981 25985.
14. Hullo MF, Auger S, Soutourina O, Barzu O, Yvon M, Danchin A Martin-Verstraete I (2007) Conversion of methionine to cysteine in Bacillus subtilis and its regulation. J Bacteriol 189 : 187 197.
15. Hung J, Cooper D, Turner MS, Walsh T Giffard PM (2003) Cystine uptake prevents production of hydrogen peroxide by Lactobacillus fermentum BR11. FEMS Microbiol Lett 227 : 93 99.
16. Kari C, Nagy Z, Kovacs P Hernadi F (1971) Mechanism of the growth inhibitory effect of cysteine on Escherichia coli. J Gen Microbiol 68 : 349 356.
17. Leichert LI, Scharf C Hecker M (2003) Global characterization of disulfide stress in Bacillus subtilis. J Bacteriol 185 : 1967 1975.
18. Liebeke M, Pother DC, van Duy N et al 2008) Depletion of thiol-containing proteins in response to quinones in Bacillus subtilis. Mol Microbiol 69 : 1513 1529.
19. Lloyd D (2006) Hydrogen sulfide: clandestine microbial messenger? Trends Microbiol 14 : 456 462.
20. Macomber L, Rensing C Imlay JA (2007) Intracellular copper does not catalyze the formation of oxidative DNA damage in Escherichia coli. J Bacteriol 189 : 1616 1626.
21. Masip L, Veeravalli K Georgiou G (2006) The many faces of glutathione in bacteria. Antioxid Redox Sign 8 : 753 762.
22. Mostertz J, Scharf C, Hecker M Homuth G (2004) Transcriptome and proteome analysis of Bacillus subtilis gene expression in response to superoxide and peroxide stress. Microbiology 150 : 497 512.
23. Newton GL, Rawat M, La Clair JJ et al 2009) Bacillithiol is an antioxidant thiol produced in bacilli. Nat Chem Biol 5 : 625 627.
24. Nguyen TT, Eiamphungporn W, Mader U et al 2009) Genome-wide responses to carbonyl electrophiles in Bacillus subtilis: control of the thiol-dependent formaldehyde dehydrogenase AdhA and cysteine proteinase YraA by the MerR-family regulator YraB (AdhR). Mol Microbiol 71 : 876 894.
25. Park S Imlay JA (2003) High levels of intracellular cysteine promote oxidative DNA damage by driving the Fenton reaction. J Bacteriol 185 : 1942 1950.
26. Pother DC, Liebeke M, Hochgrafe F et al 2009) Diamide triggers mainly S thiolations in the cytoplasmic proteomes of Bacillus subtilis and Staphylococcus aureus. J Bacteriol 191 : 7520 7530.
27. Smits WK, Dubois JYF, Bron S, van Dijl JM Kuipers OP (2005) Tricksy business: transcriptome analysis reveals the involvement of thioredoxin a in redox homeostasis, oxidative stress, sulfur metabolism, and cellular differentiation in Bacillus subtilis. J Bacteriol 187 : 3921 3930.
28. Soutourina O Martin-Verstraete I (2007) Global regulatory network of sulfur metabolism in Bacillus subtilis. Global Regulatory Networks in Bacillus subtilis (Fujita Y, ed), pp. 111 141. Transworld Research Network.
29. Soutourina O, Poupel O, Coppée JY, Danchin A, Msadek T Martin-Verstraete I (2009) CymR, the master regulator of cysteine metabolism in Staphylococcus aureus, controls host sulfur source utilization and plays a role in biofilm formation. Mol Microbiol 73 : 194 211.
30. Tanous C, Soutourina O, Raynal B et al 2008) The CymR regulator in complex with the enzyme CysK controls cysteine metabolism in Bacillus subtilis. J Biol Chem 283 : 35551 35560.
31. Turner MS, Lo R Giffard PM (2007) Inhibition of Staphylococcus aureus growth on tellurite-containing media by Lactobacillus reuteri is dependent on CyuC and thiol production. Appl Environ Microb 73 : 1005 1009.