Approaches to the assignment of 19F resonances from 3-fluorophenylalanine labeled calmodulin using solution state NMR

Journal of Biomolecular NMR

Volumn 47, Issue 2, 2010, Pages 113-123

  Kitevski LeBlanc, Julianne L ^a   Evanics, Ferenc ^a   Prosser, Scott R ^a,b  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

3 fluorophenylalanine; 19F NMR; 1H 19F NOESY HSQC; Calmodulin

Indexed keywords

3 FLUOROPHENYLALANINE; CALMODULIN; FLUORINE; PHENYLALANINE; 3-FLUOROPHENYLALANINE; DRUG DERIVATIVE; NITROGEN; PEPTIDE FRAGMENT; TRYPSIN; TYROSINE; XENOPUS PROTEIN;

ARTICLE; BIOSYNTHESIS; CALCIUM BINDING; COMPARATIVE STUDY; EXPERIMENT; MUTATIONAL ANALYSIS; NUCLEAR MAGNETIC RESONANCE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; NUCLEAR OVERHAUSER EFFECT; PRIORITY JOURNAL; PROTON NUCLEAR MAGNETIC RESONANCE; AMINO ACID SEQUENCE; ANIMAL; CHEMICAL STRUCTURE; CHEMISTRY; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; SITE DIRECTED MUTAGENESIS; SOLUTION AND SOLUBILITY; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; ANIMALS; CALMODULIN; CRYSTALLOGRAPHY, X-RAY; FLUORINE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; NITROGEN ISOTOPES; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PEPTIDE FRAGMENTS; PHENYLALANINE; SOLUTIONS; TRYPSIN; TYROSINE; XENOPUS PROTEINS;

EID: 77954690041     PISSN: 09252738     EISSN: 15735001     Source Type: Journal    
DOI: 10.1007/s10858-010-9415-y     Document Type: Article

References (42)

1. Anderluh G, Razpotnik A, Podlesek Z, Macek P, Separovic F, Norton RS (2005) Interaction of the eukaryotic pore-forming cytolysin equinatoxin Ii with model membranes: F-19 Nmr studies. J Mol Biol 347:27-39
2. Andre I, Linse S (2002) Measurement of Ca2?-binding constants of proteins and presentation of the caligator software. Anal Biochem 305:195-205
3. Baber JL, Szabo A, Tjandra N (2001) Analysis of slow interdomain motion of macromolecules using NMR relaxation data. J Am Chem Soc 123:3953-3959
4. Barbato G, Ikura M, Kay LE, Pastor RW, Bax A (1992) Backbone dynamics of calmodulin studied by N-15 relaxation using inverse detected 2-dimensional NMR-spectroscopy-The central helix is flexible. Biochemistry 31:5269-5278
5. Bertini I, Del Bianco C, Gelis I, Katsaros N, Luchinat C, Parigi G, Peana M, Provenzani A, Zoroddu MA (2004) Experimentally exploring the conformational space sampled by domain reorientation in calmodulin. Proc Natl Acad Sci U S A 101:6841-6846
6. Brokx RD, Vogel HJ (2002) In: J VH (ed) Calcium-binding proteins protocols. Humana Press Inc., Totawa, pp 183-193
7. Chambers SE, Lau EY, Gerig JT (1994) Origins of fluorine chemicalshifts in proteins. J Am Chem Soc 116:3603-3604
8. Crivici A, Ikura M (1995) Molecular and structural basis of target recognition by calmodulin. Annu Rev Biophys Biomol Struct 24:85-116
9. Danielson MA, Falke JJ (1996) Use of F-19 NMR to probe protein structure and conformational changes. Annu Rev Biophys Biomol Struct 25:163-195
10. Delaglio F, Grzesiek S, Vuister GW, Zhu G, Pfeifer J, Bax A (1995) Nmrpipe-a multidimensional spectral processing system based on unix pipes. J Biomol NMR 6:277-293
11. Drabikowski W, Kuznicki J, Grabarek Z (1977) Similarity in Ca-2?- induced changes between troponin-c and protein Activator of 30=50-cyclic nucleotide phosphodiesterase and their tryptic fragments. Biochim Biophys Acta 485:124-133
12. Drake SK, Bourret RB, Luck LA, Simon MI, Falke JJ (1993) Activation of the phosphosignaling protein chey. 1. Analysis of the phosphorylated conformation by F-19 NMR and protein engineering. J Biol Chem 268:13081-13088
13. Evanics F, Kitevski JL, Bezsonova I, Forman-Kay J, Prosser RS (2007) F-19 NMR studies of solvent exposure and peptide binding to an Sh3 domain. Biochimica Et Biophysica Acta- General Subjects 1770:221-230
14. Evenas J, Thulin E, Malmendal A, Forsen S, Carlstrom G (1997) Nmr studies of the E140q mutant of the carboxy-terminal domain of calmodulin reveal global conformational exchange in the Ca2?- saturated state. Biochemistry 36:3448-3457
15. Fallon JL, Quiocho FA (2003) A closed compact structure of native Ca2?-calmodulin. Structure 11:U1303-U1307
16. Feeney J, McCormick JE, Bauer CJ, Birdsall B, Moody CM, Starkmann BA, Young DW, Francis P, Havlin RH, Arnold WD, Oldfield E (1996) F-19 nuclear magnetic resonance chemical shifts of fluorine containing aliphatic amino acids in proteins: studies on lactobacillus casei dihydrofolate reductase containing (2s, 4s)-5-fluoroleucine. J Am Chem Soc 118:8700-8706
17. Finn BE, Evenas J, Drakenberg T, Waltho JP, Thulin E, Forsen S (1995) Calcium-induced structural-changes and domain autonomy in calmodulin. Nat Struct Biol 2:777-783
18. Gakh YG, Gakh AA, Gronenborn AM (2000) Fluorine as an NMR probe for structural studies of chemical and biological systems. Magn Reson Chem 38:551-558
19. Gerig JT (1994) Fluorine NMR of proteins. Prog Nucl Magn Reson Spectrosc 26:293-370
20. Harris RK, Becker ED, De Menezes SMC, Goodfellow R, Granger P (2002) NMR nomenclature: nuclear spin properties and conventions for chemical shifts (Iupac Recommendations 2001) (Reprinted from Pure Appl Chem, vol73, pp 1795-1818, 2001). Concepts Magn Resonance 14:326-346
21. Hoeflich KP, Ikura M (2002) Calmodulin in action: diversity in target recognition and activation mechanisms. Cell 108:739-742
22. Ikura M, Marion D, Kay LE, Shih H, Krinks M, Klee CB, Bax A (1990) Heteronuclear 3d NMR and isotopic labeling of calmodulin- towards the complete assignment of the H-1-NMR spectrum. Biochem Pharmacol 40:153-160
23. Ishida H, Takahashi K, Nakashima K, Kumaki Y, Nakata M, Hikichi K, Yazawa M (2000) Solution structures of the N-terminal domain of yeast calmodulin: Ca2?-dependent conformational change and its functional implication. Biochemistry 39:13660-13668
24. Johnson BA, Blevins RA (1994) NMR view-a computer-program for the visualization and analysis of NMR data. J Biomol NMR 4:603-614
25. Khan F, Kuprov I, Craggs TD, Hore PJ, Jackson SE (2006) F-19 NMR studies of the native and denatured states of green fluorescent protein. J Am Chem Soc 128:10729-10737
26. Kitevski-LeBlanc JL, Al-Abdul-Wahid MS, Prosser RS (2009) A mutagenesis-free approach to assignment of F-19 NMR resonances in biosynthetically labeled proteins. J Am Chem Soc 131:2054-+
27. Li HL, Frieden C (2007) Observation of sequential steps in the folding of intestinal fatty acid binding protein using a slow folding mutant and F-19 NMR. Proc Natl Acad Sci U S A 104: 11993-11998
28. Linse S, Helmersson A, Forsen S (1991) Calcium-binding to calmodulin and its globular domains. J Biol Chem 266:8050-8054
29. Loladze VV, Ermolenko DN, Makhatadze GI (2002) Thermodynamic consequences of burial of polar and non-polar amino acid residues in the protein interior. J Mol Biol 320:343-357
30. Malmendal A, Evenas J, Forsen S, Akke M (1999) Structural dynamics in the C-terminal domain of calmodulin at low calcium levels. J Mol Biol 293:883-899
31. Meyer DF, Mabuchi Y, Grabarek Z (1996) The role of Phe-92 in the Ca2?-induced conformational transition in the C-terminal domain of calmodulin. J Biol Chem 271:11284-11290
32. Okano H, Cyert MS, Ohya Y (1998) Importance of phenylalanine residues of yeast calmodulin for target binding and activations. J Biol Chem 273:26375-26382
33. Phillips L, Separovic F, Cornell BA, Barden JA, Dosremedios CG (1991) Actin dynamics studied by solid-state NMR-spectroscopy. Eur Biophys J 19:147-155
34. Richards FM (1977) Areas, volumes, packing, and protein-structure. Annu Rev Biophys Bioeng 6:151-176
35. Rinaldi PL (1983) Heteronuclear 2d-Noe spectroscopy. J Am Chem Soc 105:5167-5168
36. Torizawa T, Shimizu M, Taoka M, Miyano H, Kainosho M (2004) Efficient production of isotopically labeled proteins by cellfree synthesis: a practical protocol. J Biomol NMR 30: 311-325
37. Walsh M, Stevens FC, Kuznicki J, Drabikowski W (1977) Characterization of tryptic fragments obtained from bovine brain protein modulator of cyclic nucleotide phosphodiesterase. J Biol Chem 252:7440-7443
38. Willis MA, Bishop B, Regan L, Brunger AT (2000) Dramatic structural and thermodynamic consequences of repacking a protein's hydrophobic core. Structure 8:1319-1328
39. Woolfson DN (2001) Core-directed protein design. Curr Opin Struct Biol 11:464-471
40. Xiao GY, Parsons JF, Tesh K, Armstrong RN, Gilliland GL (1998) Conformational changes in the crystal structure of rat glutathione transferase M1-1 with global substitution of 3-fluorotyrosine for tyrosine. J Mol Biol 281:323-339
41. Yamazaki T, Formankay JD, Kay LE (1993) 2-dimensional NMR experiments for correlating C-13-Beta and H-1-delta/epsilon chemical-shifts of aromatic residues in C-13-labeled proteins via scalar couplings. J Am Chem Soc 115:11054-11055
42. Zhang M, Tanaka T, Ikura M (1995) Calcium-induced conformational transition revealed by the solution structure of apo calmodulin. Nat Struct Biol 2:758-767