Purification and characterization of a novel glucansucrase from Leuconostoc lactis EG001

Microbiological Research

Volumn 165, Issue 5, 2010, Pages 384-391

  Kim, Yong Mo ^a   Yeon, Min Ji ^a   Choi, Nack Shick ^a   Chang, Young Hyo ^a   Jung, Min Young ^a   Song, Jae Jun ^a   Kim, Joong Su ^a  

^a Korea Research Institute of Bioscience and Biotechnology (KRIBB)   (South Korea)

Author keywords

Glucansucrase; Glycosylation; L ascorbic acid; L ascorbic acid 2 glucoside

Indexed keywords

AMINO ACID SEQUENCE; DEXTRANSUCRASE; ENZYMATIC PROPERTIES; FERMENTED FOODS; GLUCANSUCRASE; GLYCOSYLATED PRODUCTS; L-ASCORBIC ACID; LACTIC ACID BACTERIA; LEUCONOSTOC; OPEN READING FRAME; OPTIMUM ACTIVITY; RECOMBINANT ENZYMES;

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; CLONING; ENZYME ACTIVITY; ENZYMES; ESCHERICHIA COLI; ESTERIFICATION; GENE ENCODING; GLYCOSYLATION; INDUSTRIAL APPLICATIONS; KETONES; LACTIC ACID; PURIFICATION;

ORGANIC ACIDS;

AMINO ACID; ANTIOXIDANT; ASCORBIC ACID; BACTERIUM; CATALYSIS; ENZYME ACTIVITY; FERMENTATION; GENE EXPRESSION; GENETIC ENGINEERING; MOLECULAR ANALYSIS; RECOMBINATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; LEUCONOSTOC; LEUCONOSTOC LACTIS; LEUCONOSTOC MESENTEROIDES;

ALTERNANSUCRASE; GLYCOSYLTRANSFERASE;

AMINO ACID SEQUENCE; ARTICLE; CLASSIFICATION; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; LEUCONOSTOC; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PHYLOGENY;

AMINO ACID SEQUENCE; BASE SEQUENCE; GLYCOSYLTRANSFERASES; LEUCONOSTOC; MOLECULAR SEQUENCE DATA; PHYLOGENY;

EID: 77954674591     PISSN: 09445013     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.micres.2009.08.005     Document Type: Article

References (22)

1. Bertrand A., Morel S., Lefoulon F., Rolland Y., Monsan P., Magali R.S. Leuconostoc mesenteroides glucansucrase synthesis of flavonoid glucosides by acceptor reactions in aqueous-organic solvents. Carbohydr. Res. 2006, 341:855-863.
2. Chang Y.H., Jung M., Park I.S., Oh H.M. Sporolactobacillus vineae sp. nov., a spore-forming lactic acid bacterium isolated from vineyard soil. Int. J. Syst. Evol. Microbiol. 2008, 58:2316-2320.
3. Dubiois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F. Colorimetric method for determination of sugars and related substances. Anal. Chem. 1956, 28:350-356.
4. Fukui K., Fukui Y., Moriyama T. Purification and properties dextransucrase and invertase from Streptococcus mutans. J. Bacteriol. 1974, 118(3):796-804.
5. Hancock R., Viola R. Biotechnological approaches for l-ascorbic acid production. Trends in Biotechnol. 2002, 20(7):299-305.
6. Kang H.K., Oh J.S., Kim D.M. Molecular characterization and expression analysis of the glucansucrase DSRWC from Weissella cibaria synthesizing a α(1-6) glucan. FEMS Microbiol. Lett. 2009, 292:33-41.
7. Kim J.H., Kim B.G., Kim J.A., Park Y.H., Lee Y.J., Lim Y.H., et al. Glycosylation of flavonoids with E. coli expressing glycosyltransferase from Xanthomonas campestris. J. Microbiol. Biotechnol. 2007, 17(3):539-542.
8. Ko J.H., Kim B.G., Ahn J.H. Glycosylation of flavonoids with a glycosyltransferase from Bacillus cereus. FEMS Microbiol. Lett. 2006, 258:263-268.
9. Kwon T.Y., Kim C.T., Lee J.H. Transglucosylation of ascorbic acid to ascorbic acid 2-glucoside by a recombinant sucrose phosphorylase from Bifidobacterium longum. Biotechnol. Lett. 2007, 29:611-615.
10. Kralj S., Van Gell Schutten G.H., Van Der Maarel M.J.E.C., Kijkhuizen L. Efficient screening methods for glucosyltransferase genes in Lactobacillus strains. Biocatal. Biotransform. 2003, 21(4-5):181-187.
11. Kyotani D., Hasegawa K., Ohishi H., Wu W., Wang L., Hasumi K. Microbial conversion of l-ascorbic acid to l-erythroascorbic acid. Biosci. Biotechnol. Biochem. 2009, 73(4):954-956.
12. Lee S.B., Nam K.C., Lee S.J., Lee J.H., Inouye K., Park K.H. Antioxidative effects of glycosyl-ascorbic acids synthesized by maltogenic amylase to reduce lipid oxidation and volatiles production in cooked chiken meat. Biosci. Biotechnol. Biochem. 2004, 68(1):36-43.
13. Nakamura S., Oku T. Bioavailability of 2-O-α-d-glucopyranosyl-l-ascorbic acid as ascorbic acid in healthy humans. Nutrition 2009, 25:686-691.
14. Meulenbeld G.H., Hartmans S. Transglycosylation by Streptococcus mutans GS-5 glucosyltransferase-D: Acceptor specificity and engineering of reaction conditions. Biotechnol. Bioeng. 2000, 70(4):363-369.
15. Meulenbeld G.H., Zuilhof H., Van Veldhuizen A., Van Den Heuvel R.H.H., Hartmans S. Enhanced (+)-catechin transglucosylating activity of Streptococcus mutans GS-5 glucosyltransferase-D due to fructose removal. Appl. Environ. Microbiol. 1999, 65(9):4141-4147.
16. Miller G.L. Use of dinitrosalicylic acid reagent for determine of reducing sugar. Anal. Chem. 1959, 31:426-427.
17. Moon Y.H., Lee J.H., Jhon D.Y., Jun W.J., Kang S.S., Sim J.G., et al. Synthesis and characterization of novel quercetin-α-d-glucopyranosides using glucansucrase from Leuconostoc mesenteroides. Enzyme Microb. Technol. 2007, 40:1124-1129.
18. Moon Y.H., Nam S.H., Kang J., Kim Y.M., Lee J.H., Kang H.K., et al. Enzymatic synthesis and characterization of arbutin glucosides using glucansucrase from Leuconostoc mesenteroides B-1299CB. Appl. Microbiol. Biotechnol. 2007, 77:559-569.
19. Seo E.S., Lee J.H., Park J.Y., Kim D.M., Han H.J., Robyt J.F. Enzymatic synthesis and anti-coagulant effect of salicin analogs by using the Leuconostoc mesenteroides glucansucrase acceptor reaction. J. Biotechnol. 2005, 117:31-38.
20. Tanaka M., Muto N., Yamamoto I. Characterization of Bacillus stearothermophilus cyclodextrin glucanotransferase in ascorbic acid 2-O-alpha-glucoside formation. Biochim. Biophys. Acta 1991, 1078(2):127-132.
21. Yamamoto I., Muto N., Murakami K., Suga S., Yamaguch H. l-ascorbic acid alpha-glucoside formed by regioselective transglucosylation with rat intestinal and rice seed alpha-glucosidases: its improved stability and structure determination. Chem. Pharm. Bull. 1990, 38(11):3020-3023.
22. Zhang H., Hu Y., Zhu C., Zhu B., Wang Y. Cloning, sequencing and expression of a dextransucrase gene (dexYG) from Leuconostoc mesenteroides. Biotechnol. Lett. 2008, 30:1441-1446.