Quantitative caveats of standard immunohistochemical procedures: Implications for optical disector-based designs

Journal of Histochemistry and Cytochemistry

Volumn 58, Issue 7, 2010, Pages 577-584

  Melvin, Neal R ^a   Sutherland, Robert J ^b  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

^b UNIVERSITY OF LETHBRIDGE   (Canada)

Author keywords

Antibody; Immunohistochemistry; Optical disector; Penetration; Stereology

Indexed keywords

ANTIBODY; FLUOROCHROME; CELL NUCLEUS ANTIGEN; FLUORESCENT DYE; MICROTUBULE ASSOCIATED PROTEIN; MTAP2 PROTEIN, RAT; NERVE GROWTH FACTOR; NERVE PROTEIN; NEUN PROTEIN, RAT; PROTEIN S 100; S 100 CALCIUM BINDING PROTEIN BETA SUBUNIT; S-100 CALCIUM-BINDING PROTEIN BETA SUBUNIT;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTIBODY DETECTION; ANTIBODY LABELING; ANTIGEN EXPRESSION; ARTICLE; CELL COUNT; CELL SHAPE; CELL SIZE; IMMUNOFLUORESCENCE; IMMUNOHISTOCHEMISTRY; INCUBATION TIME; NONHUMAN; OPTICS; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RAT; TISSUE SECTION; ANIMAL; FLUORESCENT ANTIBODY TECHNIQUE; IMMUNOLOGY; INSTRUMENTATION; LONG EVANS RAT; METABOLISM; METHODOLOGY; OPTICAL INSTRUMENTATION; PERMEABILITY; STANDARD;

ANIMALS; ANTIBODIES; ANTIGENS, NUCLEAR; FLUORESCENT ANTIBODY TECHNIQUE; FLUORESCENT DYES; MICROTUBULE-ASSOCIATED PROTEINS; NERVE GROWTH FACTORS; NERVE TISSUE PROTEINS; OPTICAL DEVICES; PERMEABILITY; RATS; RATS, LONG-EVANS; S100 PROTEINS;

EID: 77954670093     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/jhc.2009.954164     Document Type: Article

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