Plastid microsatellites for the study of genetic variability in the widespread Cephalanthera longifolia, C. damasonium and C. rubra (Neottieae, Orchidaceae), and cross-amplification in other Cephalanthera species

Botanical Journal of the Linnean Society

Volumn 163, Issue 2, 2010, Pages 181-193

  Micheneau, Claire ^a,b,d   Duffy, Karl J ^a,c,e   Smith, Rhian J ^a   Stevens, Laura J ^a   Stout, Jane C ^c   Civeyrel, Laure ^b   Cowan, Robyn S ^a   Fay, Michael F ^a  

^a JODRELL LABORATORY   (United Kingdom)

^b UNIVERSITÉ DE TOULOUSE   (France)

^c TRINITY COLLEGE DUBLIN   (Ireland)

^d UNIVERSITÉ LIBRE DE BRUXELLES   (Belgium)

^e UNIVERSITY OF KWAZULU NATAL   (South Africa)

Author keywords

Conservation genetics; Conservation management; Helleborines; Molecular markers; Orchids

Indexed keywords

ALLELE; CONSERVATION GENETICS; CONSERVATION MANAGEMENT; GENETIC MARKER; GENETIC VARIATION; INBREEDING; MONOCOTYLEDON; NOMENCLATURE; PLASTID; SAMPLING; VEGETATIVE REPRODUCTION;

CEPHALANTHERA; CEPHALANTHERA DAMASONIUM; CEPHALANTHERA LONGIFOLIA; CEPHALANTHERA RUBRA; NEOTTIEAE; ORCHIDACEAE;

EID: 77954626529     PISSN: 00244074     EISSN: 10958339     Source Type: Journal    
DOI: 10.1111/j.1095-8339.2010.01053.x     Document Type: Article

References (56)

1. Abadie J-C, Püttsepp Ü, Gebauer G, Faccio A, Bonfante P, Selosse M-A. Cephalanthera longifolia (Neottieae, Orchidaceae) is mixotrophic: a comparative study between green and nonphotosynthetic individuals. Canadian Journal of Botany 2006, 84:1462-1477.
2. Alarcón ML, Aedo C. Revisión taxonómica del género Cephalanthera (Orchidaceae) en la Península Ibérica e Islas Baleares. Anales del Jardín Botánico de Madrid 2002, 59:227-248.
3. Bateman RM, Hollingsworth PM, Squirrell J, Hollingsworth ML. Phylogenetics. Genera orchidacearum. Volume 4. Epidendroideae (Part 1) 2005, 487-495. Pridgeon AM, Cribb PJ, Chase MW, Rasmussen FN. In, eds., Oxford, Oxford University Press
4. Bateman RM, Smith RJ, Fay MF. Morphometric and population genetic analyses elucidate the origin, evolutionary significance and conservation implications of Orchis × angusticruris (O. purpurea × O. simia), a hybrid orchid new to Britain. Botanical Journal of the Linnean Society 2008, 157:687-711.
5. Bernardos S, Santos MA, Tyteca D, Amich F. Phylogenetic relationships of Mediterranean Neottieae and Orchideae (Orchidaceae) inferred from nuclear ribosomal ITS sequences. Acta Botanica Gallica 2006, 153:153-166.
6. Bidartondo MI, Read DJ. Fungal specificity bottlenecks during orchid germination and development. Molecular Ecology 2008, 17:3707-3716.
7. Bournerias M. Les orchidées de France, de Belgique et Luxembourg 1998, Paris, La Société Française d'Orchidophilie
8. Brzosko E, Wróblewska A. Genetic variation and clonal diversity in island Cephalanthera rubra populations from the Biebrza National Park, Poland. Botanical Journal of the Linnean Society 2003, 143:99-108.
9. Burns-Balogh P, Szlachetko DL, Dafni A. Evolution, pollinisation, and systematics of the tribe Neottieae (Orchidaceae). Plant Systematics and Evolution 1987, 156:91-115.
10. Byfield AJ. Cephalanthera longifolia (L.) Fritsch. Scarce plants of Britain 1994, 103-104. Stewart APearman DAPreston CD. In, eds., Peterborough, Joint Nature Conservancy Council
11. Chase MW, Hills HG. Silica gel: an ideal material for field preservation of leaf samples for DNA studies. Taxon 1991, 40:215-220.
12. Chen S, Lang K. Cephalanthera calcarata, a new saprophytic orchid from China. Acta Botanica Yunnanica 1986, 8:271-274.
13. Chung MY, Nason JD, Chung MG. Spatial genetic structure in populations of the terrestrial orchid Cephalanthera longibracteata (Orchidaceae). American Journal of Botany 2004, 91:52-57.
14. Clément JL. Connaissance des orchidées sauvages 1978, Paris, La maison rustique
15. Cribb PJ, Chen S-C. The true identity of Cephalanthera yünnanensis Hand.-Mazz. (Orchidaceae). Lindleyana 1997, 12:16-18.
16. D'Emerico S, Grünanger P, Scrugli A, Pignone D. Karyomorphological parameters and C-band distributions suggest phyletic relationships within the subtribe Limodorinae (Orchidaceae). Plant Systematics and Evolution 1999, 217:147-161.
17. Dafni A, Ivri Y. The flower biology of Cephalanthera longifolia (Orchidaceae). Pollen imitation and facultative floral mimicry. Plant Systematics and Evolution 1981, 137:224-240.
18. Darwin C. On the various contrivances by which British and foreign orchids are fertilized by insects, and on the good effects of intercrossing 1862, London, John Murray
19. Davis R. Species action plans for plants. Sword-leaved helleborine 1999, London, Plantlife
20. Delforge P. Guide des orchidées d'Europe, d'Afrique du Nord et du Proche-Orient 2005, 3rd, edn., Paris, Delachaux et Niestlé
21. Devey DS, Bateman RM, Fay MF, Hawkins JA. Genetic structure and systematic relationships within the Ophrys fuciflora aggregate (Orchidaceae: Orchidinae): high diversity in Kent and a wind-induced discontinuity bisecting the Adriatic. Annals of Botany 2009, 104:483-495.
22. Doyle JJ, Doyle JL. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochemical Bulletin, Botanical Society of America 1987, 19:11-15.
23. Dressler RL. The Neottieae in orchid classification. Lindleyana 1990, 5:102-109.
24. Duffy KJ, Kingston NE, Sayers BA, Roberts DL, Stout JC. Inferring national and regional declines of rare orchid species with probabilistic models. Conservation Biology 2009a, 23:184-195.
25. Duffy KJ, Scopece G, Cozzolino S, Fay MF, Smith RJ, Stout JC. Ecology and genetic diversity of the dense-flowered orchid, Neotinea maculata, at the centre and edge of its range. Annals of Botany 2009b, 104:507-516.
26. Fay MF, Bayer C, Alverson WS, de Bruijn AY, Chase MW. Plastid rbcL sequence data indicate a close affinity between Diegodendron and Bixa. Taxon 1998, 47:43-50.
27. Fay MF, Bone R, Cook P, Kahandawala I, Greensmith J, Harris S, Pedersen HÆ, Ingrouille MJ, Lexer C. Genetic diversity in Cypripedium calceolus (Orchidaceae) with a focus on northwestern Europe, as revealed by plastid DNA length polymorphisms. Annals of Botany 2009, 104:517-525.
28. Fay MF, Cowan RS. Plastid microsatellites in Cypripedium calceolus (Orchidaceae): genetic fingerprints from herbarium specimens. Lindleyana 2001, 16:151-156.
29. Fay MF, Cowan RS, Leitch IJ. The effects of nuclear DNA content (C-value) on the quality and utility of AFLP fingerprints. Annals of Botany 2005, 95:237-246.
30. Fay MF, Krauss SL. Orchid conservation genetics in the molecular age. Orchid conservation 2003, 91-112. Dixon KW, Kell SPBarrett RLCribb PJ. In, eds., Kota Kinabalu, Natural History Publications
31. Fay MF, Smith RJ, Zuiderduin K, Hooper E, Samuel R, Bateman RM, Chase MW. How does hybridization influence the decision making process in conservation? The genus Orchis (Orchidaceae) as a case history. Lankesteriana 2007, 7:135-137.
32. Fay MF, Thornton H, Cowan RS. Genetic fingerprinting of British populations of Cephalanthera longifolia: a project for Plantlife 1998, Kew, Royal Botanic Gardens
33. Index to plant chromosome numbers 1979, Goldblatt PJohnson DE. eds., onwards, St. Louis, MO, Missouri Botanical Garden
34. Hu A-Q, Tian H-Z, Xing F-W. Cephalanthera nanlingensis (Orchidaceae), a new species from Guangdong, China. Novon 2009, 19:56-58.
35. Julou T, Burghardt B, Gebauer G, Berveiller D, Damesin C, Selosse M-A. Mixotrophy in orchids: insights from a comparative study of green individuals and nonphotosynthetic individuals of Cephalanthera damasonium. New Phytologist 2005, 166:639-653.
36. Leake JR. The biology of myco-heterotrophic ('saprophytic') plants. New Phytologist 1994, 127:171-216.
37. Mabberley DJ. Mabberley's plant-book: a portable dictionary of plants, their classification and uses 2008, 3rd, edn., Cambridge, Cambridge University Press
38. Micheneau C. Relations phylogénétiques des Neottieae (Orchidaceae) et étude de la variabilité génétique de Cephalanthera longifoliaà l'aide de marqueurs moléculaires (séquençage et microsatellites chloroplastiques. 2002, DEA mémoire, Muséum National d'Histoire Naturelle, Paris
39. Micheneau C, Johnson SD, Fay MF. Orchid pollination: from Darwin to the present day. Botanical Journal of the Linnean Society 2009, 161:1-19.
40. Moscone EA, Samuel R, Schwarzacher T, Schweizer D, Pedrosa-Harand A. Complex rearrangements are involved in Cephalanthera (Orchidaceae) chromosome evolution. Chromosome Research 2007, 15:931-943.
41. Newman RD, Showler A. The use of copper rings to reduce losses of red helleborine Cephalanthera rubra to slug and snail herbivory in the Chiltern Hills, Buckinghamshire, England. Conservation Evidence 2007, 4:66-68.
42. Newman RD, Showler A, Harvey MC, Showler DA. Hand pollination to increase seed-set of red helleborine Cephalanthera rubra in the Chiltern Hills, Buckinghamshire, England. Conservation Evidence 2007, 4:88-93.
43. Nilsson LA. Mimesis of bellflower (Campanula) by the red helleborine orchid Cephalanthera rubra. Nature 1983, 305:799-800.
44. Parsa A. Flore de l'Iran 1951, Tehran, Ministry of Education
45. Perring FH, Farrell L. British red data book: 1. Vascular plants 1977, Lincoln, The Society for the Promotion of Nature Conservation
46. Pillon Y, Qamaruz-Zaman F, Fay MF, Hendoux F, Piquot Y. Genetic diversity and ecological differentiation in the endangered fen orchid (Liparis loeselii). Conservation Genetics 2007, 8:177-184.
47. Preston CD, Pearman DA, Dines TD. New atlas of the British and Irish flora 2002, Oxford, Oxford University Press
48. Scacchi R, De Angelis G, Corbo RM. Effect of the breeding system on the genetic structure in three Cephalanthera spp. (Orchidaceae). Plant Systematics and Evolution 1991, 176:53-61.
49. Smith SD, Cowan RS, Gregg KB, Chase MW, Maxted N, Fay MF. Genetic discontinuities among populations of Cleistes in North America (Orchidaceae, Vanilloideae). Botanical Journal of the Linnean Society 2004, 147:87-95.
50. Stace C. New flora of the British Isles 1997, 2nd, edn., Cambridge, Cambridge University Press
51. Summerhayes VS. Wild orchids of Britain 1985, 2nd, edn., London, Collins
52. Szlachetko DL, Mytnik-Ejsmont J, Margońska HB. Cephalanthera ericiflora Szlach. & Mytnik, sp. nov. (Orchidaceae, Neottioideae), a new species from Laos. Acta Societatis Botanicorum Poloniae 2008, 77:213-215.
53. Tali K, Fay MF, Bateman RM. Little genetic differentiation across Europe between early-flowering and late-flowering populations of the rapidly declining orchid Neotinea ustulata. Biological Journal of the Linnean Society 2006, 87:13-25.
54. Tully T, Le Galliard J-F. Menaces-conservation. Le Courrier de la Nature 2001, 189:33-45.
55. Vij SP, Vaidya N. Cytomorphological studies in Cephalanthera longifolia (L.) Fritsch. Journal of the Orchid Society of India 1993, 7:21-30.
56. The Board of Trustees of the Royal Botanic Gardens, Kew. 2010, http://www.kew.org/wcsp/, World Checklist of Monocotyledons, Published on the Internet; Available at: accessed 29 March 2010