Two new diophrys-like genera and their type species, apodiophrys ovalis n. g., n. sp. and heterodiophrys zhui n. g., n. sp. (Ciliophora: Euplotida), with notes on their molecular phylogeny

Journal of Eukaryotic Microbiology

Volumn 57, Issue 4, 2010, Pages 354-361

  Jiang, Jiamei ^a   Song, Weibo ^a  

^a OCEAN UNIVERSITY OF CHINA   (China)

Author keywords

Diophrys; marine ciliates; new genus; new species

Indexed keywords

PROTOZOAL DNA; RIBOSOME DNA; SEA WATER;

CILIATE; GENETIC ANALYSIS; GEOGRAPHICAL DISTRIBUTION; MOLECULAR ANALYSIS; MORPHOLOGY; NEW TAXON; PHYLOGENETICS; PHYLOGENY; TAXONOMY; TYPE SPECIMEN;

ARTICLE; CHINA; CILIATE; CLASSIFICATION; GENETICS; GROWTH, DEVELOPMENT AND AGING; ISOLATION AND PURIFICATION; MOLECULAR GENETICS; PARASITOLOGY; PHYLOGENY;

CHINA; CILIOPHORA; DNA, PROTOZOAN; DNA, RIBOSOMAL; MOLECULAR SEQUENCE DATA; PHYLOGENY; SEAWATER;

PACIFIC OCEAN; SOUTH CHINA SEA;

CILIOPHORA; DIOPHRYS; EUPLOTIDA; URONYCHIA;

EID: 77954574333     PISSN: 10665234     EISSN: 15507408     Source Type: Journal    
DOI: 10.1111/j.1550-7408.2010.00482.x     Document Type: Article

References (34)

1. Agamaliev, F. G. 1971. Complements to the fauna of psammophilic ciliates of the western coast of the Caspian Sea. Acta Protozool., 8 : 379 404. (in Russian with English summary)
2. Alekperov, I. K. 1984. New freshwater ciliates (Hypotrichida) from water bodies of Azerbaijan. Zool. Zh., 63 : 1448 1463. (in Russian with English summary)
3. Borror, A. C. 1972. Tidal marsh ciliates (Protozoa): morphology, ecology, systematics. Acta Protozool., 10 : 29 71.
4. Curds, R. C. Wu, I. C. H. 1983. A review of the Euplotidae (Hypotrichida, Ciliophora). Bull. Br. Mus. Nat. Hist. (Zool.), 44 : 191 247.
5. Dragesco, J. 1963. Compléments à la connaissance des ciliés mésopsammiques de Roscoff. II. Heterotriches III. Hypotriches. Cah. Biol. Mar., 4 : 251 275.
6. Dujardin, F. 1841. Histoire Naturelle des Zoophytes Infusoires. Paris. 678 p.
7. Eddy, S. R. 2003. HMMER user's guide. Available at (accessed November 11, 2009).
8. Fauré-Fremiet, E. 1964. Les ciliés hypotrichs reptrocursifs. Arch. Zool. Exp. Gén., 104 : 65 74.
9. Gao, S., Chen, Z., Shao, C., Long, H., Al-Rasheid, K. A. S. Song, W. 2008. Reconsideration of the phylogenetic position of Frontonia-related Peniculia (Ciliophora, Protozoa) inferred from the small subunit ribosomal RNA gene sequences. Acta Protozool., 47 : 47 54.
10. Guindon, S. Gascuel, O. 2003. A simple, fast and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst. Biol., 52 : 696 704.
11. Hall, T. A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 9598NT. Nucl. Acids Symp. Ser., 41 : 95 98.
12. Hartwig, E. 1973. Die Ciliaten des Gezeiten-Sandstrandes der Nordseeinsel Sylt. I. Systematik. Akad. Wiss. Lit. (Mainz) Math. Naturwiss. Kl. Mikrofauna Meersbod., 18 : 387 453.
13. Hill, B. F. Borror, A. C. 1992. Redefinition of the genera Diophrys and Paradiophrys and establishment of the genus Diophryopsis n. g. (Ciliophora, Hypotrichida): implication for the species problem. J. Protozool., 39 : 144 153.
14. Hu, X. 2008. Cortical structure in non-dividing and cortical morphogenesis in dividing Diophrys japonica spec. nov. (Ciliophora, Euplotida) with notes of morphological variation. Eur. J. Protistol., 41 : 115 129.
15. Jankowski, A. W. 1978. Systematic revision of the class Polyhemenophorea (Spirotricha), morphology, systematics and evolution. Zool. Inst. Leningrad, Akad. Nauk SSSR, 86 : 39 40. (in Russian)
16. Jankowski, A. W. 1979. Systematics and phylogeny of the order Hypotrichida Stein, 1859 (Protozoa, Ciliophora). Trudy Zool. Inst. Leningrad, Akad. Nauk SSSR, 86 : 48 85. (in Russian with English summary)
17. Kahl, A. 1932. Urtiere oder Protozoa I. Wimpertiere oder Ciliata (Infusoria) III. Spirotricha. Tierwelt Dtl., 25 : 399 650.
18. Lynn, D. H. 2008. The Ciliated Protozoa, Characterization, Classification, and Guide to the Literature. 3rd ed. Springer
19. Medlin, L., Elwood, H. L., Stickel, S. Sogin, M. L. 1988. The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene, 71 : 491 499.
20. Miao, M., Wang, Y., Li, L., Al-Rasheid, K. A. S. Song, W. 2009. Molecular phylogeny of the scuticociliate Philaster (Protozoa, Ciliophora) based on SSU rRNA gene sequences information, with description of a new species P. apodigitiformis sp. n. Syst. Biodiversity, 7 : 381 388.
21. Nylander, J. A. A. 2004. MrModeltest Version 2. Distributed by the author, Department of Systematic Zoology, Evolutionary Biology Centre, Uppsala University
22. Petz, W., Song, W. Wilbert, N. 1995. Taxonomy and ecology of the ciliate fauna (Protozoa, Ciliophora) in the endopagial and pelagial of the Weddell Sea, Antarctica. Stapfia, 40 : 1 223.
23. Raikov, I. B. Kovaleva, V. G. 1968. Complements to the fauna of psammobiotic ciliates of the Japan Sea (Prosjet Gulf). Acta Protozool., 6 : 309 333.
24. Ruinen, J. 1938. Notizen über Ciliaten aus konzentrierten Salzgewässern. Zool. Medel., 20 : 243 256.
25. Shao, C., Zhang, Q., Al-Rasheid, K. A. S., Warren, A. Song, W. 2010. Ontogenesis and molecular phylogeny of the marine ciliate Diophryopsis hystrix: implications for the systematics of the Diophrys-complex (Ciliophora, Spirotrichea, Euplotida). J. Eukaryot. Microbiol., 57 : 33 39.
26. Song, W. Packroff, G. 1997. Taxonomische Untersuchungen an marinen Ciliaten aus China mit Beschreibungen von 2 neuen Arten, Strombidium globosaneum nov. spec. und Strombidium platum nov. spec. (Protozoa, Ciliophora). Arch. Protistenkd., 147 : 331 360.
27. Song, W., Shao, C., Yi, Z., Li, L., Warren, A., Al-Rasheid, K. Yang, J. 2009. The morphology, morphogenesis and SS rRNA gene sequence of a new marine ciliate, Diophrys apoligothrix spec. nov. (Ciliophora; Euplotida). Eur. J. Protistol., 45 : 38 50.
28. Song, W., Wilbert, N., Al-Rasheid, A., Warren, A., Shao, C., Long, H., Yi, Z. Li, L. 2007. Redescriptions of two marine hypotrichous ciliates, Diophrys irmgard and D. hystrix (Ciliophora, Euplotida), with a brief revision of the genus Diophrys. J. Eukaryot. Microbiol., 54 : 283 296.
29. Swofford, D. L. 2002. PAUP: Phylogenetic Analysis Using Parsimony. Sinauer
30. Wilbert, N. 1975. Eine verbesserte Technik der Protargolimprägnation für Ciliaten. Mikrokosmos, 64 : 171 179.
31. Wilbert, N. Song, W. 2008. A further study on littoral ciliates (Protozoa, Ciliophora) near King George Island, Antarctica, with description of a new genus and seven new species. J. Nat. Hist., 42 : 979 1012.
32. Wuyts, J., Van de Peer, Y., Winkelmans, T. De Wachter, R. 2002. The European database on small subunit ribosomal RNA. Nucl. Acids Res., 30 : 183 185.
33. Yi, Z., Song, W., Clamp, J. C., Chen, Z., Gao, S. Zhang, Q. 2009. Reconsideration of systematic relationships within the order Euplotida (Protista, Ciliophora) using new sequences of the gene coding for small-subunit rRNA and testing the use of combined data sets to construct phylogenies of the Diophrys-complex. Mol. Phylogenet. Evol., 50 : 599 607.
34. Yi, Z., Song, W., Warren, A., Roberts, D., Al-Rasheid, K. A. S., Chen, Z., Al-Farraj, S. A. Hu, X. 2008. A molecular phylogenetic investigation of Pseudoamphisiella and Parabirojimia (Protozoa, Ciliophora, Spirotrichea), two genera with ambiguous systematic positions. Eur. J. Protistol., 44 : 45 53.