Flow cytometric fluorescence pulse width analysis of etoposide-induced nuclear enlargement in HCT116 cells

Biotechnology Letters

Volumn 32, Issue 8, 2010, Pages 1045-1052

  Kang, Kyungsu ^a,b   Lee, Saet Byoul ^a   Yoo, Ji Hye ^a   Nho, Chu Won ^a  

^a Korea Institute of Science and Technology   (South Korea)

^b Seoul National University   (South Korea)

Author keywords

Cell cycle; Etoposide; Flow cytometry; Fluorescence; Nuclear size; Pulse width

Indexed keywords

ANTICANCER DRUG; CELL CYCLE; DNA CONTENT; ETOPOSIDE; EXPERIMENTAL MODELS; FLUORESCENCE PULSE; FLUORESCENCE SIGNALS; MICROSCOPIC OBSERVATIONS; PROPIDIUM IODIDE; PULSE WIDTH; S-PHASE; VEHICLE CONTROL; W-VALUE;

CELLS; CONTROL SYSTEM SYNTHESIS; CYTOLOGY; FLOW CYTOMETRY; FLUORESCENCE; NUCLEAR PHYSICS;

BIOINFORMATICS;

ANTINEOPLASTIC AGENT; ETOPOSIDE;

ARTICLE; CELL CULTURE; CELL CYCLE; CELL NUCLEUS; CELL STRAIN HCT116; CELL SURVIVAL; DRUG EFFECT; FLOW CYTOMETRY; FLUORESCENCE; HUMAN; METHODOLOGY; ORGAN SIZE; WESTERN BLOTTING; DRUG EFFECTS; HCT116 CELL LINE; PROCEDURES;

ANTINEOPLASTIC AGENTS, PHYTOGENIC; BLOTTING, WESTERN; CELL CYCLE; CELL NUCLEUS; CELL SURVIVAL; CELLS, CULTURED; ETOPOSIDE; FLOW CYTOMETRY; FLUORESCENCE; HCT116 CELLS; HUMANS; ORGAN SIZE;

EID: 77954543287     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0277-x     Document Type: Article

References (15)

1. Dedov VN, Dedova IV, Nicholson GA (2003) Inhibition of topoisomerase II overrides the G2/M check points of the cell cycle in EBV-lymphocytes. Apoptosis 8: 399-406.
2. Fadeel B, Hassan Z, Hellstrom-Lindberg E, Henter JI, Orrenius S, Zhivotovsky B (1999) Cleavage of Bcl-2 is an early event in chemotherapy-induced apoptosis of human myeloid leukemia cells. Leukemia 13: 719-728.
3. Hoffman RA (2009) Pulse width for particle sizing. Curr Protoc Cytom Chapter 1: Unit 1. 23.
4. Kang K, Lee HJ, Kim CY, Lee SB, Tunsag J, Batsuren D, Nho CW (2007) The chemopreventive effects of Saussurea salicifolia through induction of apoptosis and phase II detoxification enzyme. Biol Pharm Bull 30: 2352-2359.
5. Kang K, Lee SB, Jung SH, Cha KH, Park WD, Sohn YC, Nho CW (2009) Tectoridin, a poor ligand of estrogen receptor alpha, exerts its estrogenic effects via an ERK-dependent pathway. Mol Cells 27: 351-357.
6. Kirkhus B, Glaso M, Clausen OP (1992) Multivariate flow cytometry of epidermal regeneration provoked by a skin irritant and a tumor promoter. Cytometry 13: 267-274.
7. Leary JF, Todd P, Wood JC, Jett JH (1979) Laser flow cytometric light scatter and fluorescence pulse width and pulse rise-time sizing of mammalian cells. J Histochem Cytochem 27: 315-320.
8. Min DJ, Moskowitz NP, Brownstein C, Lee H, Horton TM, Carroll WL (2006) Diverse pathways mediate chemotherapy-induced cell death in acute lymphoblastic leukemia cell lines. Apoptosis 11: 1977-1986.
9. Parnaik VK (2008) Role of nuclear lamins in nuclear organization, cellular signaling, and inherited diseases. Int Rev Cell Mol Biol 266: 157-206.
10. Perez C, Vilaboa NE, Garcia-Bermejo L, de Blas E, Creighton AM, Aller P (1997) Differentiation of U-937 promonocytic cells by etoposide and ICRF-193, two antitumour DNA topoisomerase II inhibitors with different mechanisms of action. J Cell Sci 110: 337-343.
11. Rello-Varona S, Gamez A, Moreno V, Stockert JC, Cristobal J, Pacheco M, Canete M, Juarranz A, Villanueva A (2006) Metaphase arrest and cell death induced by etoposide on HeLa cells. Int J Biochem Cell Biol 38: 2183-2195.
12. Rudolf E, Cervinka M (2003) Topoisomerases and tubulin inhibitors: a promising combination for cancer treatment. Curr Med Chem Anticancer Agents 3: 421-429.
13. Wersto RP, Chrest FJ, Leary JF, Morris C, Stetler-Stevenson MA, Gabrielson E (2001) Doublet discrimination in DNA cell-cycle analysis. Cytometry 46: 296-306.
14. Young IT (1977) Proof without prejudice: use of the Kolmogorov-Smirnov test for the analysis of histograms from flow systems and other sources. J Histochem Cytochem 25: 935-941.
15. Zhu H, Miao ZH, Huang M, Feng JM, Zhang ZX, Lu JJ, Cai YJ, Tong LJ, Xu YF, Qian XH, Ding J (2009) Naphthalimides induce G(2) arrest through the ATM-activated Chk2-executed pathway in HCT116 cells. Neoplasia 11: 1226-1234.