Engineering of dye-mediated dehydrogenase property of fructosyl amino acid oxidases by site-directed mutagenesis studies of its putative proton relay system

Biotechnology Letters

Volumn 32, Issue 8, 2010, Pages 1123-1129

  Kim, Seungsu ^a   Nibe, Eri ^a   Ferri, Stefano ^a   Tsugawa, Wakako ^a   Sode, Koji ^a,b  

^a TOKYO UNIVERSITY OF AGRICULTURE AND TECHNOLOGY   (Japan)

^b Ultizyme International Ltd   (Japan)

Author keywords

Biosensor; Dehydrogenase; Flavoprotein; Fructosyl amino acid oxidase (FAOD); Glycated protein; Proton relay system (PRS)

Indexed keywords

AMADORIASE; AMPEROMETRIC; ASN RESIDUES; ASPERGILLUS FUMIGATUS; CATALYTIC ABILITY; CONSERVED RESIDUES; DEHYDROGENASE ACTIVITY; ELECTRON ACCEPTOR; ELECTRON MEDIATOR; FLAVOENZYMES; FLAVOPROTEIN; FRUCTOSYL AMINO ACID OXIDASE; MARINE YEASTS; MODEL COMPOUND; OXYGEN REACTIVITY; POTENTIAL UTILITY; PROTEIN PROTONS; PROTON-RELAY SYSTEMS; SITE DIRECTED MUTAGENESIS;

BIOSENSORS; ELECTRONS; ENZYME SENSORS; MUTAGENESIS; ORGANIC ACIDS; OXYGEN; PROTEINS; PROTONS;

AMINO ACIDS;

AMADORIASE; OXIDOREDUCTASE; PROTON;

AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS; CHEMICAL STRUCTURE; CHEMISTRY; ENZYMOLOGY; GENETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; PICHIA; POLYACRYLAMIDE GEL ELECTROPHORESIS; SITE DIRECTED MUTAGENESIS; X RAY CRYSTALLOGRAPHY;

AMINO ACID OXIDOREDUCTASES; AMINO ACID SEQUENCE; ASPERGILLUS; CLONING, MOLECULAR; CRYSTALLOGRAPHY, X-RAY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; OXIDOREDUCTASES; PICHIA; PROTONS;

ASPERGILLUS FUMIGATUS; EUKARYOTA; FUNGI; PICHIA; PICHIA SP.;

EID: 77954535620     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0267-z     Document Type: Article

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